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Crystallization and preliminary X-ray data analysis of beta-alanine synthase from Drosophila melanogaster

Lundgren, Stina; Andersen, Birgit LU ; Piskur, Jure LU and Dobritzsch, Doreen (2007) In Acta Crystallographica. Section F: Structural Biology and Crystallization Communications2005-01-01+01:002014-01-01+01:00 63. p.874-877
Abstract
beta-Alanine synthase catalyzes the last step in the reductive degradation pathway for uracil and thymine, which represents the main clearance route for the widely used anticancer drug 5-fluorouracil. Crystals of the recombinant enzyme from Drosophila melanogaster, which is closely related to the human enzyme, were obtained by the hanging-drop vapour-diffusion method. They diffracted to 3.3 angstrom at a synchrotron-radiation source, belong to space group C2 (unit-cell parameters a = 278.9, b = 95.0, c = 199.3 angstrom, beta = 125.8 degrees) and contain 8-10 molecules per asymmetric unit.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Crystallographica. Section F: Structural Biology and Crystallization Communications2005-01-01+01:002014-01-01+01:00
volume
63
pages
874 - 877
publisher
Wiley-Blackwell
external identifiers
  • wos:000250012400014
  • scopus:34848926196
ISSN
2053-230X
DOI
10.1107/S1744309107042984
language
English
LU publication?
yes
id
2d052e19-92d7-4243-86ea-c0f75e3f0906 (old id 655559)
date added to LUP
2007-12-17 15:28:51
date last changed
2017-08-27 05:40:04
@article{2d052e19-92d7-4243-86ea-c0f75e3f0906,
  abstract     = {beta-Alanine synthase catalyzes the last step in the reductive degradation pathway for uracil and thymine, which represents the main clearance route for the widely used anticancer drug 5-fluorouracil. Crystals of the recombinant enzyme from Drosophila melanogaster, which is closely related to the human enzyme, were obtained by the hanging-drop vapour-diffusion method. They diffracted to 3.3 angstrom at a synchrotron-radiation source, belong to space group C2 (unit-cell parameters a = 278.9, b = 95.0, c = 199.3 angstrom, beta = 125.8 degrees) and contain 8-10 molecules per asymmetric unit.},
  author       = {Lundgren, Stina and Andersen, Birgit and Piskur, Jure and Dobritzsch, Doreen},
  issn         = {2053-230X},
  language     = {eng},
  pages        = {874--877},
  publisher    = {Wiley-Blackwell},
  series       = {Acta Crystallographica. Section F: Structural Biology and Crystallization Communications2005-01-01+01:002014-01-01+01:00},
  title        = {Crystallization and preliminary X-ray data analysis of beta-alanine synthase from Drosophila melanogaster},
  url          = {http://dx.doi.org/10.1107/S1744309107042984},
  volume       = {63},
  year         = {2007},
}