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Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes

Broholm, Christa; Olsson, Anders Henrik; Perfilyev, Alexander LU ; Gillberg, Linn LU ; Hansen, Ninna Schiøler; Ali, Ashfaq LU ; Mortensen, Brynjulf; Ling, Charlotte LU and Vaag, Allan LU (2016) In Epigenomics 8(12). p.1601-1617
Abstract

Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire... (More)

Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
adipogenesis, adiponectin, DNA methylation, epigenetics, stem cells
in
Epigenomics
volume
8
issue
12
pages
17 pages
publisher
Future Medicine Ltd.
external identifiers
  • scopus:85002944734
  • wos:000390675400004
ISSN
1750-1911
DOI
10.2217/epi-2016-0077
language
English
LU publication?
yes
id
655811c8-e449-4214-8138-959205118aeb
date added to LUP
2016-12-28 10:41:04
date last changed
2017-09-18 11:33:32
@article{655811c8-e449-4214-8138-959205118aeb,
  abstract     = {<p>Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.</p>},
  author       = {Broholm, Christa and Olsson, Anders Henrik and Perfilyev, Alexander and Gillberg, Linn and Hansen, Ninna Schiøler and Ali, Ashfaq and Mortensen, Brynjulf and Ling, Charlotte and Vaag, Allan},
  issn         = {1750-1911},
  keyword      = {adipogenesis,adiponectin,DNA methylation,epigenetics,stem cells},
  language     = {eng},
  month        = {12},
  number       = {12},
  pages        = {1601--1617},
  publisher    = {Future Medicine Ltd.},
  series       = {Epigenomics},
  title        = {Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes},
  url          = {http://dx.doi.org/10.2217/epi-2016-0077},
  volume       = {8},
  year         = {2016},
}