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Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes

Broholm, Christa ; Olsson, Anders Henrik ; Perfilyev, Alexander LU orcid ; Gillberg, Linn LU ; Hansen, Ninna Schiøler ; Ali, Ashfaq LU orcid ; Mortensen, Brynjulf ; Ling, Charlotte LU orcid and Vaag, Allan LU (2016) In Epigenomics 8(12). p.1601-1617
Abstract

Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire... (More)

Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
adipogenesis, adiponectin, DNA methylation, epigenetics, stem cells
in
Epigenomics
volume
8
issue
12
pages
17 pages
publisher
Future Medicine Ltd.
external identifiers
  • pmid:27854126
  • wos:000390675400004
  • scopus:85002944734
ISSN
1750-1911
DOI
10.2217/epi-2016-0077
language
English
LU publication?
yes
id
655811c8-e449-4214-8138-959205118aeb
date added to LUP
2016-12-28 10:41:04
date last changed
2025-10-14 12:55:34
@article{655811c8-e449-4214-8138-959205118aeb,
  abstract     = {{<p>Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.</p>}},
  author       = {{Broholm, Christa and Olsson, Anders Henrik and Perfilyev, Alexander and Gillberg, Linn and Hansen, Ninna Schiøler and Ali, Ashfaq and Mortensen, Brynjulf and Ling, Charlotte and Vaag, Allan}},
  issn         = {{1750-1911}},
  keywords     = {{adipogenesis; adiponectin; DNA methylation; epigenetics; stem cells}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{12}},
  pages        = {{1601--1617}},
  publisher    = {{Future Medicine Ltd.}},
  series       = {{Epigenomics}},
  title        = {{Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes}},
  url          = {{http://dx.doi.org/10.2217/epi-2016-0077}},
  doi          = {{10.2217/epi-2016-0077}},
  volume       = {{8}},
  year         = {{2016}},
}