Modified General Primer PCR System for Sensitive Detection of Multiple Types of Oncogenic Human Papillomavirus
(2009) In Journal of Clinical Microbiology 47(3). p.541-546- Abstract
- Human papillomavirus (HPV) infection is a necessary cause of cervical cancer and cervical dysplasia. Accurate and sensitive genotyping of multiple oncogenic HPVs is essential for a multitude of both clinical and research uses. We developed a modified general primer (MGP) PCR system with five forward and five reverse consensus primers. The MGP system was compared to the classical HPV general primer system GP5 +/6 + using a proficiency panel with HPV plasmid dilutions as well as cervical samples from 592 women with low-grade cytological abnormalities. The reference method (GP5 +/6 +) had the desirable high sensitivity (five copies/PCR) for five oncogenic HPV types (HPV type 16 [HPV-16], HPV-18, HPV-56, HPV-59, and HPV-66). The MGP system was... (More)
- Human papillomavirus (HPV) infection is a necessary cause of cervical cancer and cervical dysplasia. Accurate and sensitive genotyping of multiple oncogenic HPVs is essential for a multitude of both clinical and research uses. We developed a modified general primer (MGP) PCR system with five forward and five reverse consensus primers. The MGP system was compared to the classical HPV general primer system GP5 +/6 + using a proficiency panel with HPV plasmid dilutions as well as cervical samples from 592 women with low-grade cytological abnormalities. The reference method (GP5 +/6 +) had the desirable high sensitivity (five copies/PCR) for five oncogenic HPV types (HPV type 16 [HPV-16], HPV-18, HPV-56, HPV-59, and HPV-66). The MGP system was able to detect all 14 oncogenic HPV types at five copies/PCR. In the clinical samples, the MGP system detected a significantly higher proportion of women with more than two concomitant HPV infections than did the GP5 +/6 + system (102/592 women compared to 42/592 women). MGP detected a significantly greater number of infections with HPV-16, -18, -31, -33, -35, -39, -42, -43, -45, -51, -52, -56, -58, and -70 than did GP5 +/6 +. In summary, the MGP system primers allow a more sensitive amplification of most of the HPV types that are established as oncogenic and had an improved ability to detect multiple concomitant HPV infections. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1371121
- author
- Söderlund Strand, Anna LU ; Carlson, Joyce LU and Dillner, Joakim LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Clinical Microbiology
- volume
- 47
- issue
- 3
- pages
- 541 - 546
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000263818800004
- pmid:19144817
- scopus:62749172924
- pmid:19144817
- ISSN
- 1098-660X
- DOI
- 10.1128/JCM.02007-08
- language
- English
- LU publication?
- yes
- id
- 656017e3-00df-48b7-a2f8-54f84ab0115c (old id 1371121)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19144817?dopt=Abstract
- date added to LUP
- 2016-04-01 11:58:17
- date last changed
- 2022-03-20 21:39:10
@article{656017e3-00df-48b7-a2f8-54f84ab0115c, abstract = {{Human papillomavirus (HPV) infection is a necessary cause of cervical cancer and cervical dysplasia. Accurate and sensitive genotyping of multiple oncogenic HPVs is essential for a multitude of both clinical and research uses. We developed a modified general primer (MGP) PCR system with five forward and five reverse consensus primers. The MGP system was compared to the classical HPV general primer system GP5 +/6 + using a proficiency panel with HPV plasmid dilutions as well as cervical samples from 592 women with low-grade cytological abnormalities. The reference method (GP5 +/6 +) had the desirable high sensitivity (five copies/PCR) for five oncogenic HPV types (HPV type 16 [HPV-16], HPV-18, HPV-56, HPV-59, and HPV-66). The MGP system was able to detect all 14 oncogenic HPV types at five copies/PCR. In the clinical samples, the MGP system detected a significantly higher proportion of women with more than two concomitant HPV infections than did the GP5 +/6 + system (102/592 women compared to 42/592 women). MGP detected a significantly greater number of infections with HPV-16, -18, -31, -33, -35, -39, -42, -43, -45, -51, -52, -56, -58, and -70 than did GP5 +/6 +. In summary, the MGP system primers allow a more sensitive amplification of most of the HPV types that are established as oncogenic and had an improved ability to detect multiple concomitant HPV infections.}}, author = {{Söderlund Strand, Anna and Carlson, Joyce and Dillner, Joakim}}, issn = {{1098-660X}}, language = {{eng}}, number = {{3}}, pages = {{541--546}}, publisher = {{American Society for Microbiology}}, series = {{Journal of Clinical Microbiology}}, title = {{Modified General Primer PCR System for Sensitive Detection of Multiple Types of Oncogenic Human Papillomavirus}}, url = {{http://dx.doi.org/10.1128/JCM.02007-08}}, doi = {{10.1128/JCM.02007-08}}, volume = {{47}}, year = {{2009}}, }