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Low-light-induced formation of semicrystalline photosystem II arrays in higher plant chloroplast

Kirchhoff, Helmut; Haase, Winfried; Wegner, Sandra; Danielsson, Ravi LU ; Ackermann, Ralf and Albertsson, Per-Åke LU (2007) In Biochemistry 46(39). p.11169-11176
Abstract
Remodeling of photosynthetic machinery induced by growing spinach plants under low light intensities reveals an up-regulation of light-harvesting complexes and down-regulation of photosystem II and cytochrome b(6)f complexes in intact thylakoids and isolated grana membranes. The antenna size of PSII increased by 40-60% as estimated by fluorescence induction and LHCII/PSII stoichiometry. These low-light-induced changes in the protein composition were accompanied by the formation of ordered particle arrays in the exoplasmic fracture face in grana thylakoids detected by freeze-fracture electron microscopy. Most likely these highly ordered arrays consist of PSII complexes. A statistical analysis of the particles in these structures shows that... (More)
Remodeling of photosynthetic machinery induced by growing spinach plants under low light intensities reveals an up-regulation of light-harvesting complexes and down-regulation of photosystem II and cytochrome b(6)f complexes in intact thylakoids and isolated grana membranes. The antenna size of PSII increased by 40-60% as estimated by fluorescence induction and LHCII/PSII stoichiometry. These low-light-induced changes in the protein composition were accompanied by the formation of ordered particle arrays in the exoplasmic fracture face in grana thylakoids detected by freeze-fracture electron microscopy. Most likely these highly ordered arrays consist of PSII complexes. A statistical analysis of the particles in these structures shows that the distance of neighboring complexes in the same row is 18.0 urn, the separation between two rows is 23.7 nm, and the angle between the particle axis and the row is 26 degrees. On the basis of structural information on the photosystem II supercomplex, a model on the supramolecular arrangement was generated predicting that two neighboring complexes share a trimeric light-harvesting complex. It was suggested that the supramolecular reorganization in ordered arrays in low-light grana thylakoids is a strategy to overcome potential diffusion problems in this crowded membrane. Furthermore, the occurrence of a hexagonal phase of the lipid monogalactosyldiacylglycerol in grana membranes of low-light-adapted plants could trigger the rearrangement by changing the lateral membrane pressure. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemistry
volume
46
issue
39
pages
11169 - 11176
publisher
The American Chemical Society
external identifiers
  • wos:000249726500016
  • scopus:34848816208
ISSN
0006-2960
DOI
10.1021/bi700748y
language
English
LU publication?
yes
id
51ea487f-a38a-4eee-a2e6-6a6ed8ed9063 (old id 656212)
date added to LUP
2007-12-12 14:22:05
date last changed
2017-10-29 03:34:10
@article{51ea487f-a38a-4eee-a2e6-6a6ed8ed9063,
  abstract     = {Remodeling of photosynthetic machinery induced by growing spinach plants under low light intensities reveals an up-regulation of light-harvesting complexes and down-regulation of photosystem II and cytochrome b(6)f complexes in intact thylakoids and isolated grana membranes. The antenna size of PSII increased by 40-60% as estimated by fluorescence induction and LHCII/PSII stoichiometry. These low-light-induced changes in the protein composition were accompanied by the formation of ordered particle arrays in the exoplasmic fracture face in grana thylakoids detected by freeze-fracture electron microscopy. Most likely these highly ordered arrays consist of PSII complexes. A statistical analysis of the particles in these structures shows that the distance of neighboring complexes in the same row is 18.0 urn, the separation between two rows is 23.7 nm, and the angle between the particle axis and the row is 26 degrees. On the basis of structural information on the photosystem II supercomplex, a model on the supramolecular arrangement was generated predicting that two neighboring complexes share a trimeric light-harvesting complex. It was suggested that the supramolecular reorganization in ordered arrays in low-light grana thylakoids is a strategy to overcome potential diffusion problems in this crowded membrane. Furthermore, the occurrence of a hexagonal phase of the lipid monogalactosyldiacylglycerol in grana membranes of low-light-adapted plants could trigger the rearrangement by changing the lateral membrane pressure.},
  author       = {Kirchhoff, Helmut and Haase, Winfried and Wegner, Sandra and Danielsson, Ravi and Ackermann, Ralf and Albertsson, Per-Åke},
  issn         = {0006-2960},
  language     = {eng},
  number       = {39},
  pages        = {11169--11176},
  publisher    = {The American Chemical Society},
  series       = {Biochemistry},
  title        = {Low-light-induced formation of semicrystalline photosystem II arrays in higher plant chloroplast},
  url          = {http://dx.doi.org/10.1021/bi700748y},
  volume       = {46},
  year         = {2007},
}