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Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases

Sandrini, Michael LU ; Soderbom, Fredrik; Mikkelsen, Nils Egil and Piskur, Jure LU (2007) In Journal of Molecular Biology 369(3). p.653-664
Abstract
The salvage of deoxyribonucleosides in the social amoeba Dictyostelium discoideum, which has an extremely A + T-rich genome, was investigated. All native deoxyribonucleosides were phosphorylated by D. discoideum cell extracts and we subcloned three deoxyribonucleoside kinase (dNK) encoding genes. D. discoideum thymidine kinase was similar to the human thymidine kinase 1 and was specific for thymidine with a k(m) of 5.1 mu M. The other two cloned kinases were phylogenetically closer to bacterial deoxyribonucleoside kinases than to the eukaryotic enzymes. D. discoideum deoxyadenosine kinase (DddAK) had a K-m for deoxyadenosine of 22.7 mu M and a k(cat) of 3.7 s(-1) and could not efficiently phosphorylate any other native deoxyribonucleoside.... (More)
The salvage of deoxyribonucleosides in the social amoeba Dictyostelium discoideum, which has an extremely A + T-rich genome, was investigated. All native deoxyribonucleosides were phosphorylated by D. discoideum cell extracts and we subcloned three deoxyribonucleoside kinase (dNK) encoding genes. D. discoideum thymidine kinase was similar to the human thymidine kinase 1 and was specific for thymidine with a k(m) of 5.1 mu M. The other two cloned kinases were phylogenetically closer to bacterial deoxyribonucleoside kinases than to the eukaryotic enzymes. D. discoideum deoxyadenosine kinase (DddAK) had a K-m for deoxyadenosine of 22.7 mu M and a k(cat) of 3.7 s(-1) and could not efficiently phosphorylate any other native deoxyribonucleoside. D. discoideum deoxyguanosine kinase was also a purine-specific kinase and phosphorylated significantly only deoxyguanosine, with a K-m of 1.4 mu M and a k(cat) of 3 s(-1). The two purine-specific deoxyribonucleoside kinases could represent ancient enzymes present in the common ancestor of bacteria and eukaryotes but remaining only in a few eukaryote lineages. The narrow substrate specificity of the D. discoideum dNKs reflects the biased genome composition and we attempted to explain the strict preference of DddAK for deoxyadenosine by modeling the active center with different substrates. Apart from its native substrate, deoxyadenosine, DddAK efficiently phosphorylated fludarabine. Hence, DddAK could be used in the enzymatic production of fludarabine monophosphate, a drug used in the treatment of chronic lymphocytic leukemia. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
green, chemistry, Dictyostelium, salvage, deoxyribonucleoside kinase, fludarabine
in
Journal of Molecular Biology
volume
369
issue
3
pages
653 - 664
publisher
Elsevier
external identifiers
  • wos:000246810500006
  • scopus:34248216123
ISSN
1089-8638
DOI
10.1016/j.jmb.2007.03.053
language
English
LU publication?
yes
id
6e74326d-446c-49eb-b385-7f48e53e345d (old id 657470)
date added to LUP
2007-12-14 14:05:15
date last changed
2017-01-01 07:00:31
@article{6e74326d-446c-49eb-b385-7f48e53e345d,
  abstract     = {The salvage of deoxyribonucleosides in the social amoeba Dictyostelium discoideum, which has an extremely A + T-rich genome, was investigated. All native deoxyribonucleosides were phosphorylated by D. discoideum cell extracts and we subcloned three deoxyribonucleoside kinase (dNK) encoding genes. D. discoideum thymidine kinase was similar to the human thymidine kinase 1 and was specific for thymidine with a k(m) of 5.1 mu M. The other two cloned kinases were phylogenetically closer to bacterial deoxyribonucleoside kinases than to the eukaryotic enzymes. D. discoideum deoxyadenosine kinase (DddAK) had a K-m for deoxyadenosine of 22.7 mu M and a k(cat) of 3.7 s(-1) and could not efficiently phosphorylate any other native deoxyribonucleoside. D. discoideum deoxyguanosine kinase was also a purine-specific kinase and phosphorylated significantly only deoxyguanosine, with a K-m of 1.4 mu M and a k(cat) of 3 s(-1). The two purine-specific deoxyribonucleoside kinases could represent ancient enzymes present in the common ancestor of bacteria and eukaryotes but remaining only in a few eukaryote lineages. The narrow substrate specificity of the D. discoideum dNKs reflects the biased genome composition and we attempted to explain the strict preference of DddAK for deoxyadenosine by modeling the active center with different substrates. Apart from its native substrate, deoxyadenosine, DddAK efficiently phosphorylated fludarabine. Hence, DddAK could be used in the enzymatic production of fludarabine monophosphate, a drug used in the treatment of chronic lymphocytic leukemia.},
  author       = {Sandrini, Michael and Soderbom, Fredrik and Mikkelsen, Nils Egil and Piskur, Jure},
  issn         = {1089-8638},
  keyword      = {green,chemistry,Dictyostelium,salvage,deoxyribonucleoside kinase,fludarabine},
  language     = {eng},
  number       = {3},
  pages        = {653--664},
  publisher    = {Elsevier},
  series       = {Journal of Molecular Biology},
  title        = {Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases},
  url          = {http://dx.doi.org/10.1016/j.jmb.2007.03.053},
  volume       = {369},
  year         = {2007},
}