L-Arabinose metabolism in Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012: influence of sugar and oxygen on product formation
(2007) In Applied Microbiology and Biotechnology 75(2). p.303-310- Abstract
- L-Arabinose utilization by the yeasts Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012 was investigated in aerobic batch cultures and compared, under similar conditions, to D-glucose and D-xylose metabolism. At high aeration levels, only biomass was formed from all the three sugars. When oxygen became limited, ethanol was produced from D-glucose, demonstrating a fermentative pathway in these yeasts. However, pentoses were essentially respired and, under oxygen limitation, the respective polyols accumulated-arabitol from L-arabinose and xylitol from D-xylose. Different L-arabinose concentrations and oxygen conditions were tested to better understand L-arabinose metabolism. P. guilliermondii PYCC 3012 excreted... (More)
- L-Arabinose utilization by the yeasts Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012 was investigated in aerobic batch cultures and compared, under similar conditions, to D-glucose and D-xylose metabolism. At high aeration levels, only biomass was formed from all the three sugars. When oxygen became limited, ethanol was produced from D-glucose, demonstrating a fermentative pathway in these yeasts. However, pentoses were essentially respired and, under oxygen limitation, the respective polyols accumulated-arabitol from L-arabinose and xylitol from D-xylose. Different L-arabinose concentrations and oxygen conditions were tested to better understand L-arabinose metabolism. P. guilliermondii PYCC 3012 excreted considerably more arabitol from L-arabinose (and also xylitol from D-xylose) than C arabinofermentans PYCC 5603(T). In contrast to the latter, P guilliermondii PYCC 3012 did not produce any traces of ethanol in complex L-arabinose (80 g/l) medium under oxygen-limited conditions. Neither sustained growth nor active metabolism was observed under anaerobiosis. This study demonstrates, for the first time, the oxygen dependence of metabolite and product formation in L-arabinose-assimilating yeasts. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/657593
- author
- Fonseca, Cesar ; Spencer-Martins, Isabel and Hahn-Hägerdal, Bärbel LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Hemicellulose, yeast, fermentation conditions, L-Arabinose, pentose, Candida arabinofermentans, Pichia guilliermondii
- in
- Applied Microbiology and Biotechnology
- volume
- 75
- issue
- 2
- pages
- 303 - 310
- publisher
- Springer
- external identifiers
-
- wos:000246842500008
- scopus:33947254105
- ISSN
- 1432-0614
- DOI
- 10.1007/s00253-006-0830-7
- language
- English
- LU publication?
- yes
- id
- cc837718-f65a-4b5d-878f-d41bd1d8c745 (old id 657593)
- date added to LUP
- 2016-04-01 16:24:53
- date last changed
- 2022-02-12 22:02:44
@article{cc837718-f65a-4b5d-878f-d41bd1d8c745,
abstract = {{L-Arabinose utilization by the yeasts Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012 was investigated in aerobic batch cultures and compared, under similar conditions, to D-glucose and D-xylose metabolism. At high aeration levels, only biomass was formed from all the three sugars. When oxygen became limited, ethanol was produced from D-glucose, demonstrating a fermentative pathway in these yeasts. However, pentoses were essentially respired and, under oxygen limitation, the respective polyols accumulated-arabitol from L-arabinose and xylitol from D-xylose. Different L-arabinose concentrations and oxygen conditions were tested to better understand L-arabinose metabolism. P. guilliermondii PYCC 3012 excreted considerably more arabitol from L-arabinose (and also xylitol from D-xylose) than C arabinofermentans PYCC 5603(T). In contrast to the latter, P guilliermondii PYCC 3012 did not produce any traces of ethanol in complex L-arabinose (80 g/l) medium under oxygen-limited conditions. Neither sustained growth nor active metabolism was observed under anaerobiosis. This study demonstrates, for the first time, the oxygen dependence of metabolite and product formation in L-arabinose-assimilating yeasts.}},
author = {{Fonseca, Cesar and Spencer-Martins, Isabel and Hahn-Hägerdal, Bärbel}},
issn = {{1432-0614}},
keywords = {{Hemicellulose; yeast; fermentation conditions; L-Arabinose; pentose; Candida arabinofermentans; Pichia guilliermondii}},
language = {{eng}},
number = {{2}},
pages = {{303--310}},
publisher = {{Springer}},
series = {{Applied Microbiology and Biotechnology}},
title = {{L-Arabinose metabolism in Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012: influence of sugar and oxygen on product formation}},
url = {{http://dx.doi.org/10.1007/s00253-006-0830-7}},
doi = {{10.1007/s00253-006-0830-7}},
volume = {{75}},
year = {{2007}},
}