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L-Arabinose metabolism in Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012: influence of sugar and oxygen on product formation

Fonseca, Cesar; Spencer-Martins, Isabel and Hahn-Hägerdal, Bärbel LU (2007) In Applied Microbiology and Biotechnology 75(2). p.303-310
Abstract
L-Arabinose utilization by the yeasts Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012 was investigated in aerobic batch cultures and compared, under similar conditions, to D-glucose and D-xylose metabolism. At high aeration levels, only biomass was formed from all the three sugars. When oxygen became limited, ethanol was produced from D-glucose, demonstrating a fermentative pathway in these yeasts. However, pentoses were essentially respired and, under oxygen limitation, the respective polyols accumulated-arabitol from L-arabinose and xylitol from D-xylose. Different L-arabinose concentrations and oxygen conditions were tested to better understand L-arabinose metabolism. P. guilliermondii PYCC 3012 excreted... (More)
L-Arabinose utilization by the yeasts Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012 was investigated in aerobic batch cultures and compared, under similar conditions, to D-glucose and D-xylose metabolism. At high aeration levels, only biomass was formed from all the three sugars. When oxygen became limited, ethanol was produced from D-glucose, demonstrating a fermentative pathway in these yeasts. However, pentoses were essentially respired and, under oxygen limitation, the respective polyols accumulated-arabitol from L-arabinose and xylitol from D-xylose. Different L-arabinose concentrations and oxygen conditions were tested to better understand L-arabinose metabolism. P. guilliermondii PYCC 3012 excreted considerably more arabitol from L-arabinose (and also xylitol from D-xylose) than C arabinofermentans PYCC 5603(T). In contrast to the latter, P guilliermondii PYCC 3012 did not produce any traces of ethanol in complex L-arabinose (80 g/l) medium under oxygen-limited conditions. Neither sustained growth nor active metabolism was observed under anaerobiosis. This study demonstrates, for the first time, the oxygen dependence of metabolite and product formation in L-arabinose-assimilating yeasts. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Hemicellulose, yeast, fermentation conditions, L-Arabinose, pentose, Candida arabinofermentans, Pichia guilliermondii
in
Applied Microbiology and Biotechnology
volume
75
issue
2
pages
303 - 310
publisher
Springer
external identifiers
  • wos:000246842500008
  • scopus:33947254105
ISSN
1432-0614
DOI
10.1007/s00253-006-0830-7
language
English
LU publication?
yes
id
cc837718-f65a-4b5d-878f-d41bd1d8c745 (old id 657593)
date added to LUP
2007-12-10 13:46:43
date last changed
2017-11-19 04:10:11
@article{cc837718-f65a-4b5d-878f-d41bd1d8c745,
  abstract     = {L-Arabinose utilization by the yeasts Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012 was investigated in aerobic batch cultures and compared, under similar conditions, to D-glucose and D-xylose metabolism. At high aeration levels, only biomass was formed from all the three sugars. When oxygen became limited, ethanol was produced from D-glucose, demonstrating a fermentative pathway in these yeasts. However, pentoses were essentially respired and, under oxygen limitation, the respective polyols accumulated-arabitol from L-arabinose and xylitol from D-xylose. Different L-arabinose concentrations and oxygen conditions were tested to better understand L-arabinose metabolism. P. guilliermondii PYCC 3012 excreted considerably more arabitol from L-arabinose (and also xylitol from D-xylose) than C arabinofermentans PYCC 5603(T). In contrast to the latter, P guilliermondii PYCC 3012 did not produce any traces of ethanol in complex L-arabinose (80 g/l) medium under oxygen-limited conditions. Neither sustained growth nor active metabolism was observed under anaerobiosis. This study demonstrates, for the first time, the oxygen dependence of metabolite and product formation in L-arabinose-assimilating yeasts.},
  author       = {Fonseca, Cesar and Spencer-Martins, Isabel and Hahn-Hägerdal, Bärbel},
  issn         = {1432-0614},
  keyword      = {Hemicellulose,yeast,fermentation conditions,L-Arabinose,pentose,Candida arabinofermentans,Pichia guilliermondii},
  language     = {eng},
  number       = {2},
  pages        = {303--310},
  publisher    = {Springer},
  series       = {Applied Microbiology and Biotechnology},
  title        = {L-Arabinose metabolism in Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012: influence of sugar and oxygen on product formation},
  url          = {http://dx.doi.org/10.1007/s00253-006-0830-7},
  volume       = {75},
  year         = {2007},
}