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Comparison of immunohistochemical mesothelial biomarkers in paired biopsies and effusion cytology cell blocks from pleural mesothelioma

Mansour, Mohammed S I LU orcid ; Huseinzade, Adela ; Seidal, Tomas ; Hejny, Kim ; Maty, Athar ; Taheri-Eilagh, Fereshteh ; Mager, Ulrich ; Dejmek, Annika LU ; Dobra, Katalin LU and Brunnström, Hans LU orcid (2023) In Cytopathology 34(5). p.456-465
Abstract

OBJECTIVE: Traditionally, the diagnosis of pleural mesothelioma is based on histological material. Minimally invasive effusion cytology specimens are an alternative that, like biopsies, require ancillary analyses. Validation of immunohistochemical (IHC) analyses on cytology, including the surrogate markers for molecular alterations BAP1 and MTAP, is of interest.

METHODS: IHC for eight different markers was performed on 59 paired formalin-fixed, paraffin-embedded pleural biopsies and pleural effusion cell blocks with mesothelioma. Immunoreactivity in ≥10% of tumour cells was considered positive/preserved. The concordance between histological and cytological materials was assessed.

RESULTS: The overall percentage of agreement... (More)

OBJECTIVE: Traditionally, the diagnosis of pleural mesothelioma is based on histological material. Minimally invasive effusion cytology specimens are an alternative that, like biopsies, require ancillary analyses. Validation of immunohistochemical (IHC) analyses on cytology, including the surrogate markers for molecular alterations BAP1 and MTAP, is of interest.

METHODS: IHC for eight different markers was performed on 59 paired formalin-fixed, paraffin-embedded pleural biopsies and pleural effusion cell blocks with mesothelioma. Immunoreactivity in ≥10% of tumour cells was considered positive/preserved. The concordance between histological and cytological materials was assessed.

RESULTS: The overall percentage of agreement between the histological epithelioid component in 58 biopsies and paired cell blocks was 93% for calretinin, 98% for CK5, 97% for podoplanin, 90% for WT1, 86% for EMA, 100% for desmin, 91% for BAP1, and 72% for MTAP. For 11 cases with biphasic or sarcomatoid histology, the concordance between cytology and the histological sarcomatoid component was low for calretinin, CK5, and WT1 (all ≤45%). For the whole cohort, loss of both BAP1 and MTAP was seen in 40% while both markers were preserved in 11% of the biopsies for epithelioid histology. The corresponding numbers were 54% and 8%, respectively, for the paired cell blocks.

CONCLUSIONS: Generally, a high concordance for IHC staining was seen between paired biopsies and pleural effusion cell blocks from mesotheliomas, but the somewhat lower agreement for WT1, EMA, and especially MTAP calls for further investigation and local quality assurance. The lower concordance for the sarcomatoid subtype for some markers may indicate biological differences.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cytopathology
volume
34
issue
5
pages
456 - 465
publisher
Wiley-Blackwell
external identifiers
  • pmid:37337638
  • scopus:85162940896
ISSN
1365-2303
DOI
10.1111/cyt.13265
project
Improvement of diagnostic and prognostic cytopathology of lung cancer and malignant mesothelioma.
Biomarkers in mesothelioma and non-small cell lung cancer: Investigation of cytological specimens with correlation to histology
language
English
LU publication?
yes
id
65957cd0-0df6-42d0-a3ba-7831aa4c8642
date added to LUP
2023-07-27 21:14:10
date last changed
2024-05-19 02:20:04
@article{65957cd0-0df6-42d0-a3ba-7831aa4c8642,
  abstract     = {{<p>OBJECTIVE: Traditionally, the diagnosis of pleural mesothelioma is based on histological material. Minimally invasive effusion cytology specimens are an alternative that, like biopsies, require ancillary analyses. Validation of immunohistochemical (IHC) analyses on cytology, including the surrogate markers for molecular alterations BAP1 and MTAP, is of interest.</p><p>METHODS: IHC for eight different markers was performed on 59 paired formalin-fixed, paraffin-embedded pleural biopsies and pleural effusion cell blocks with mesothelioma. Immunoreactivity in ≥10% of tumour cells was considered positive/preserved. The concordance between histological and cytological materials was assessed.</p><p>RESULTS: The overall percentage of agreement between the histological epithelioid component in 58 biopsies and paired cell blocks was 93% for calretinin, 98% for CK5, 97% for podoplanin, 90% for WT1, 86% for EMA, 100% for desmin, 91% for BAP1, and 72% for MTAP. For 11 cases with biphasic or sarcomatoid histology, the concordance between cytology and the histological sarcomatoid component was low for calretinin, CK5, and WT1 (all ≤45%). For the whole cohort, loss of both BAP1 and MTAP was seen in 40% while both markers were preserved in 11% of the biopsies for epithelioid histology. The corresponding numbers were 54% and 8%, respectively, for the paired cell blocks.</p><p>CONCLUSIONS: Generally, a high concordance for IHC staining was seen between paired biopsies and pleural effusion cell blocks from mesotheliomas, but the somewhat lower agreement for WT1, EMA, and especially MTAP calls for further investigation and local quality assurance. The lower concordance for the sarcomatoid subtype for some markers may indicate biological differences.</p>}},
  author       = {{Mansour, Mohammed S I and Huseinzade, Adela and Seidal, Tomas and Hejny, Kim and Maty, Athar and Taheri-Eilagh, Fereshteh and Mager, Ulrich and Dejmek, Annika and Dobra, Katalin and Brunnström, Hans}},
  issn         = {{1365-2303}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{5}},
  pages        = {{456--465}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Cytopathology}},
  title        = {{Comparison of immunohistochemical mesothelial biomarkers in paired biopsies and effusion cytology cell blocks from pleural mesothelioma}},
  url          = {{http://dx.doi.org/10.1111/cyt.13265}},
  doi          = {{10.1111/cyt.13265}},
  volume       = {{34}},
  year         = {{2023}},
}