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Identification of discrepancies in grain quality and grain protein composition through avenin proteins of oat after an effort to increase protein content

SUNILKUMAR, BINDU LU and Tareke, Eden LU (2016) In Agriculture & Food Security 5(7).
Abstract
Background: Oat prolamin (avenin) is a family of proteins that contain several polymorphic components. The high
interspecific variability of avenin among cultivars, electrophoretic patterns have been used for grain protein comparison
and species relationships. Therefore, the present study was carried out to evaluate the effect of environment on
avenin constitution following an effort to increase protein content of oat using different approaches.
Results: The avenin electrophoretic patterns of 25 samples resulting from 20 oat cultivars and 5 mutated oat lines
grown or developed in different fields and greenhouses were compared. Quantification of total proteins and SDSPAGE
analysis of avenin protein extracts were... (More)
Background: Oat prolamin (avenin) is a family of proteins that contain several polymorphic components. The high
interspecific variability of avenin among cultivars, electrophoretic patterns have been used for grain protein comparison
and species relationships. Therefore, the present study was carried out to evaluate the effect of environment on
avenin constitution following an effort to increase protein content of oat using different approaches.
Results: The avenin electrophoretic patterns of 25 samples resulting from 20 oat cultivars and 5 mutated oat lines
grown or developed in different fields and greenhouses were compared. Quantification of total proteins and SDSPAGE
analysis of avenin protein extracts were carried out. Different pattern of avenin allele in electrophoretic gel
allowed the comparison within and between groups. This analysis shows that the cultivars obtained from Swedish
fields had differences in the number of alleles compared with the parental cultivar, so were not stable across sites. The
study revealed that oats treated with nitrogen and the selected mutated lines showed increase in protein concentration,
with consistent avenin loci to the parental line, so these lines are now being used as potential candidates for
developing high-protein oat lines in the future.
Conclusions: Our results provide useful information, on the stability of modifications, for breeders aiming to increasing
the content and nutritional value of oat protein. The study will increase our knowledge in the stability of different
modifications and thus to accordingly select oat lines with stable and improved nutritional values.
Keywords: Avenin, Mutation, Nitrogen, Geographical location, Electrophoresis (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Agriculture & Food Security
volume
5
issue
7
publisher
BioMed Central
external identifiers
  • scopus:85006066550
ISSN
2048-7010
DOI
10.1186/s40066-016-0056-6
language
English
LU publication?
yes
id
65d9e291-19b6-4c75-9fbc-390092476941
date added to LUP
2016-09-20 15:39:14
date last changed
2017-04-19 09:57:34
@article{65d9e291-19b6-4c75-9fbc-390092476941,
  abstract     = {Background: Oat prolamin (avenin) is a family of proteins that contain several polymorphic components. The high<br/>interspecific variability of avenin among cultivars, electrophoretic patterns have been used for grain protein comparison<br/>and species relationships. Therefore, the present study was carried out to evaluate the effect of environment on<br/>avenin constitution following an effort to increase protein content of oat using different approaches.<br/>Results: The avenin electrophoretic patterns of 25 samples resulting from 20 oat cultivars and 5 mutated oat lines<br/>grown or developed in different fields and greenhouses were compared. Quantification of total proteins and SDSPAGE<br/>analysis of avenin protein extracts were carried out. Different pattern of avenin allele in electrophoretic gel<br/>allowed the comparison within and between groups. This analysis shows that the cultivars obtained from Swedish<br/>fields had differences in the number of alleles compared with the parental cultivar, so were not stable across sites. The<br/>study revealed that oats treated with nitrogen and the selected mutated lines showed increase in protein concentration,<br/>with consistent avenin loci to the parental line, so these lines are now being used as potential candidates for<br/>developing high-protein oat lines in the future.<br/>Conclusions: Our results provide useful information, on the stability of modifications, for breeders aiming to increasing<br/>the content and nutritional value of oat protein. The study will increase our knowledge in the stability of different<br/>modifications and thus to accordingly select oat lines with stable and improved nutritional values.<br/>Keywords: Avenin, Mutation, Nitrogen, Geographical location, Electrophoresis},
  articleno    = {7},
  author       = {SUNILKUMAR, BINDU and Tareke, Eden },
  issn         = {2048-7010},
  language     = {eng},
  month        = {05},
  number       = {7},
  publisher    = {BioMed Central},
  series       = {Agriculture & Food Security},
  title        = {Identification of discrepancies in grain quality and grain protein composition through avenin proteins of oat after an effort to increase protein content},
  url          = {http://dx.doi.org/10.1186/s40066-016-0056-6},
  volume       = {5},
  year         = {2016},
}