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A water activity control system for enzymatic reactions in organic media

Hagström, Anna LU ; Adlercreutz, Patrick LU and Mattiasson, Bo LU (2007) In Biotechnology and Bioengineering 97(2). p.235-241
Abstract
A water activity control system for enzymatic synthesis in organic media, for litre-scale reactors has been constructed. Water activity, a(w), is a key factor when using enzymes in non-conventional media and the optimum value varies for different enzymes. The control system consists of a water activity sensor in the headspace of a jacketed glass reactor (equipped with narrow steel tubes to introduce air), gas-washing bottles containing blue silica gel (a(w) = 0) and water (a(w) = 1), a PC to monitor water activity and a programmable logic controller (PLC) to control the water activity. The system was evaluted by adjusting water activity in the medium, with a defviation from the set point less than +/- 0.05. Synthesis of cetyl palmitate,... (More)
A water activity control system for enzymatic synthesis in organic media, for litre-scale reactors has been constructed. Water activity, a(w), is a key factor when using enzymes in non-conventional media and the optimum value varies for different enzymes. The control system consists of a water activity sensor in the headspace of a jacketed glass reactor (equipped with narrow steel tubes to introduce air), gas-washing bottles containing blue silica gel (a(w) = 0) and water (a(w) = 1), a PC to monitor water activity and a programmable logic controller (PLC) to control the water activity. The system was evaluted by adjusting water activity in the medium, with a defviation from the set point less than +/- 0.05. Synthesis of cetyl palmitate, under controlled water activity and catalysed by two different lipase preparations, namely, Novozym (R) 435 (immobilised Candida anarctica lipase B) and immobilised Candida rugosa lipase, were also performed. Novozym (R) 435 catalyses reactions very well at extremely low water activity while C. rugosa lipase shows low activity for a(w) < 0.05. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
enzyme, water activity, organic media, process control
in
Biotechnology and Bioengineering
volume
97
issue
2
pages
235 - 241
publisher
John Wiley & Sons
external identifiers
  • wos:000246434700004
  • scopus:34249776675
ISSN
1097-0290
DOI
10.1002/bit.21229
language
English
LU publication?
yes
id
9406d775-ad9f-4c9c-b394-a3bde964518d (old id 660582)
date added to LUP
2007-12-19 13:39:22
date last changed
2017-01-01 05:09:50
@article{9406d775-ad9f-4c9c-b394-a3bde964518d,
  abstract     = {A water activity control system for enzymatic synthesis in organic media, for litre-scale reactors has been constructed. Water activity, a(w), is a key factor when using enzymes in non-conventional media and the optimum value varies for different enzymes. The control system consists of a water activity sensor in the headspace of a jacketed glass reactor (equipped with narrow steel tubes to introduce air), gas-washing bottles containing blue silica gel (a(w) = 0) and water (a(w) = 1), a PC to monitor water activity and a programmable logic controller (PLC) to control the water activity. The system was evaluted by adjusting water activity in the medium, with a defviation from the set point less than +/- 0.05. Synthesis of cetyl palmitate, under controlled water activity and catalysed by two different lipase preparations, namely, Novozym (R) 435 (immobilised Candida anarctica lipase B) and immobilised Candida rugosa lipase, were also performed. Novozym (R) 435 catalyses reactions very well at extremely low water activity while C. rugosa lipase shows low activity for a(w) &lt; 0.05.},
  author       = {Hagström, Anna and Adlercreutz, Patrick and Mattiasson, Bo},
  issn         = {1097-0290},
  keyword      = {enzyme,water activity,organic media,process control},
  language     = {eng},
  number       = {2},
  pages        = {235--241},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {A water activity control system for enzymatic reactions in organic media},
  url          = {http://dx.doi.org/10.1002/bit.21229},
  volume       = {97},
  year         = {2007},
}