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Effect of the electrical currents generated by the intestinal smooth muscle layers on pancreatic enzyme activity: An in vitro study

Dabek, Marta; Podgurniak, Pawel; Piedra, Jose L. Valverde; Szymanczyk, Sylwia; Filip, Rafal; Wojtasz-Pajak, Anna; Werpachowska, Eliza; Podgurniak, Malgorzata and Pierzynowski, Stefan LU (2007) In Bioelectromagnetics 28(4). p.275-280
Abstract
Gut enzymes in the small intestine are exposed to extremely low electrical currents (ELEC) generated by the smooth muscle. In the present study, the in vitro tests were undertaken to evaluate the effect of these electric currents on the activity of the proteolytic pancreatic digestive enzymes. A simulator generating the typical electrical activity of pig gut was used for these studies. The electric current emitted by the simulator was transmitted to the samples, containing enzyme and its substrate, using platinum plate electrodes. All samples were incubated at 37 degrees C for I h. The changes in optical density, corresponding to enzyme activity, in samples stimulated for I h with ELEC was compared with that not exposed to ELEC. The... (More)
Gut enzymes in the small intestine are exposed to extremely low electrical currents (ELEC) generated by the smooth muscle. In the present study, the in vitro tests were undertaken to evaluate the effect of these electric currents on the activity of the proteolytic pancreatic digestive enzymes. A simulator generating the typical electrical activity of pig gut was used for these studies. The electric current emitted by the simulator was transmitted to the samples, containing enzyme and its substrate, using platinum plate electrodes. All samples were incubated at 37 degrees C for I h. The changes in optical density, corresponding to enzyme activity, in samples stimulated for I h with ELEC was compared with that not exposed to ELEC. The obtained results show that the electrical current with the characteristics of the myoelectrical migrating complex (MMC) has an influence on pancreatic enzyme activity. Increased endopeptidase and reduced exopeptidase activity was noticed in samples treated with ELEC. This observation can be of important as analyzed factors which can alter enzymatic activity of the gut, can thus also affect feed/food digestibility. Bioelectromagnetics 28:275-280, 2007. (c) 2007 Wiley-Liss, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
enzyme activity, myoelectrical activity, endogenous electric current
in
Bioelectromagnetics
volume
28
issue
4
pages
275 - 280
publisher
John Wiley & Sons
external identifiers
  • wos:000246258600004
  • scopus:34249111406
ISSN
0197-8462
DOI
10.1002/bem.20263
language
English
LU publication?
yes
id
c124af54-aeeb-4989-8f23-e72dae1d1838 (old id 662776)
date added to LUP
2007-12-06 12:00:44
date last changed
2017-01-01 04:37:17
@article{c124af54-aeeb-4989-8f23-e72dae1d1838,
  abstract     = {Gut enzymes in the small intestine are exposed to extremely low electrical currents (ELEC) generated by the smooth muscle. In the present study, the in vitro tests were undertaken to evaluate the effect of these electric currents on the activity of the proteolytic pancreatic digestive enzymes. A simulator generating the typical electrical activity of pig gut was used for these studies. The electric current emitted by the simulator was transmitted to the samples, containing enzyme and its substrate, using platinum plate electrodes. All samples were incubated at 37 degrees C for I h. The changes in optical density, corresponding to enzyme activity, in samples stimulated for I h with ELEC was compared with that not exposed to ELEC. The obtained results show that the electrical current with the characteristics of the myoelectrical migrating complex (MMC) has an influence on pancreatic enzyme activity. Increased endopeptidase and reduced exopeptidase activity was noticed in samples treated with ELEC. This observation can be of important as analyzed factors which can alter enzymatic activity of the gut, can thus also affect feed/food digestibility. Bioelectromagnetics 28:275-280, 2007. (c) 2007 Wiley-Liss, Inc.},
  author       = {Dabek, Marta and Podgurniak, Pawel and Piedra, Jose L. Valverde and Szymanczyk, Sylwia and Filip, Rafal and Wojtasz-Pajak, Anna and Werpachowska, Eliza and Podgurniak, Malgorzata and Pierzynowski, Stefan},
  issn         = {0197-8462},
  keyword      = {enzyme activity,myoelectrical activity,endogenous electric current},
  language     = {eng},
  number       = {4},
  pages        = {275--280},
  publisher    = {John Wiley & Sons},
  series       = {Bioelectromagnetics},
  title        = {Effect of the electrical currents generated by the intestinal smooth muscle layers on pancreatic enzyme activity: An in vitro study},
  url          = {http://dx.doi.org/10.1002/bem.20263},
  volume       = {28},
  year         = {2007},
}