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Electroenzymatic reactions with oxygen on laccase-modified electrodes in anhydrous (pure) organic solvent

Yaropolov, A.; Shleev, Sergey LU ; Zaitseva, E.; Emnéus, Jenny LU ; Marko-Varga, György LU and Gorton, Lo LU (2007) In Bioelectrochemistry 70(2). p.199-204
Abstract
The electroenzymatic reactions of Trametes hirsuta laccase in the pure organic solvent dimethyl sulfoxide (DMSO) have been investigated within the framework for potential use as a catalytic reaction scheme for oxygen reduction. The bioelectrochemical characteristics of laccase were investigated in two different ways: (i) by studying the electroreduction of oxygen in anhydrous DMSO via a direct electron transfer mechanism without proton donors and (ii) by doing the same experiments in the presence of laccase substrates, which display in pure organic solvents both the properties of electron donors as well as the properties of weak acids. The results obtained with laccase in anhydrous DMSO were compared with those obtained previously in... (More)
The electroenzymatic reactions of Trametes hirsuta laccase in the pure organic solvent dimethyl sulfoxide (DMSO) have been investigated within the framework for potential use as a catalytic reaction scheme for oxygen reduction. The bioelectrochemical characteristics of laccase were investigated in two different ways: (i) by studying the electroreduction of oxygen in anhydrous DMSO via a direct electron transfer mechanism without proton donors and (ii) by doing the same experiments in the presence of laccase substrates, which display in pure organic solvents both the properties of electron donors as well as the properties of weak acids. The results obtained with laccase in anhydrous DMSO were compared with those obtained previously in aqueous buffer. It was shown that in the absence of proton donors under oxygenated conditions, formation of superoxide anion radicals is prevented at bare glassy carbon and graphite electrodes with adsorbed laccase. The influence of the time for drying the laccase solution at the electrode surface on the electroreduction of oxygen was studied. Investigating the electroenzymatic oxidation reaction of catechol and hydroquinone in DMSO reveals the formation of various intermediates of the substrates with different electrochemical activity under oxygenated conditions. The influence of the content of aqueous buffer in the organic solvent on the electrochemical behaviour of hydroquinone/1,4-benzoquinone couple was also studied. (C) 2006 Elsevier B.V. All fights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
anhydrous organic solvent, laccase, electroenzymatic reaction, dimethyl, sulfoxide
in
Bioelectrochemistry
volume
70
issue
2
pages
199 - 204
publisher
Elsevier
external identifiers
  • wos:000246254500001
  • scopus:33947725907
ISSN
1878-562X
DOI
10.1016/j.bioelechem.2006.07.005
language
English
LU publication?
yes
id
3790f05f-f160-4f55-9dda-271fa99f01bc (old id 662786)
date added to LUP
2008-01-03 11:13:12
date last changed
2017-02-19 03:36:58
@article{3790f05f-f160-4f55-9dda-271fa99f01bc,
  abstract     = {The electroenzymatic reactions of Trametes hirsuta laccase in the pure organic solvent dimethyl sulfoxide (DMSO) have been investigated within the framework for potential use as a catalytic reaction scheme for oxygen reduction. The bioelectrochemical characteristics of laccase were investigated in two different ways: (i) by studying the electroreduction of oxygen in anhydrous DMSO via a direct electron transfer mechanism without proton donors and (ii) by doing the same experiments in the presence of laccase substrates, which display in pure organic solvents both the properties of electron donors as well as the properties of weak acids. The results obtained with laccase in anhydrous DMSO were compared with those obtained previously in aqueous buffer. It was shown that in the absence of proton donors under oxygenated conditions, formation of superoxide anion radicals is prevented at bare glassy carbon and graphite electrodes with adsorbed laccase. The influence of the time for drying the laccase solution at the electrode surface on the electroreduction of oxygen was studied. Investigating the electroenzymatic oxidation reaction of catechol and hydroquinone in DMSO reveals the formation of various intermediates of the substrates with different electrochemical activity under oxygenated conditions. The influence of the content of aqueous buffer in the organic solvent on the electrochemical behaviour of hydroquinone/1,4-benzoquinone couple was also studied. (C) 2006 Elsevier B.V. All fights reserved.},
  author       = {Yaropolov, A. and Shleev, Sergey and Zaitseva, E. and Emnéus, Jenny and Marko-Varga, György and Gorton, Lo},
  issn         = {1878-562X},
  keyword      = {anhydrous organic solvent,laccase,electroenzymatic reaction,dimethyl,sulfoxide},
  language     = {eng},
  number       = {2},
  pages        = {199--204},
  publisher    = {Elsevier},
  series       = {Bioelectrochemistry},
  title        = {Electroenzymatic reactions with oxygen on laccase-modified electrodes in anhydrous (pure) organic solvent},
  url          = {http://dx.doi.org/10.1016/j.bioelechem.2006.07.005},
  volume       = {70},
  year         = {2007},
}