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Genome survey sequencing of the wine spoilage yeast Dekkera (Brettanomyces) bruxellensis

Woolfit, Megan; Rozpedowska, Elzbieta LU ; Piskur, Jure LU and Wolfe, Kenneth H. (2007) In Eukaryotic Cell 6(4). p.721-733
Abstract
The hemiascomycete yeast Dekkera bruxellensis, also known as Brettanomyces bruxellensis, is a major cause of wine spoilage worldwide. Wines infected with D. bruxellensis develop distinctive, unpleasant aromas due to volatile phenols produced by this species, which is highly ethanol tolerant and facultatively anaerobic. Despite its importance, however, D. bruxellensis has been poorly genetically characterized until now. We performed genome survey sequencing of a wine strain of D. bruxellensis to obtain 0.4x coverage of the genome. We identified approximately 3,000 genes, whose products averaged 49% amino acid identity to their Saccharomyces cerevisiae orthologs, with similar intron contents. Maximum likelihood phylogenetic analyses suggest... (More)
The hemiascomycete yeast Dekkera bruxellensis, also known as Brettanomyces bruxellensis, is a major cause of wine spoilage worldwide. Wines infected with D. bruxellensis develop distinctive, unpleasant aromas due to volatile phenols produced by this species, which is highly ethanol tolerant and facultatively anaerobic. Despite its importance, however, D. bruxellensis has been poorly genetically characterized until now. We performed genome survey sequencing of a wine strain of D. bruxellensis to obtain 0.4x coverage of the genome. We identified approximately 3,000 genes, whose products averaged 49% amino acid identity to their Saccharomyces cerevisiae orthologs, with similar intron contents. Maximum likelihood phylogenetic analyses suggest that the relationship between D. brurellensis, S. cerevisiae, and Candida albicans is close to a trichotomy. The estimated rate of chromosomal rearrangement in D. bruxellensis is slower than that calculated for C albicans, while its rate of amino acid evolution is somewhat higher. The proteome of D. bruxellensis is enriched for transporters and genes involved in nitrogen and lipid metabolism, among other functions, which may reflect adaptations to its low-nutrient, high-ethanol niche. We also identified an adenyl deaminase gene that has high similarity to a gene in bacteria of the Burkholderia cepacia species complex and appears to be the result of horizontal gene transfer. These data provide a resource for further analyses of the population genetics and evolution of D. bruxellensis and of the genetic bases of its physiological capabilities. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Eukaryotic Cell
volume
6
issue
4
pages
721 - 733
publisher
American Society for Microbiology
external identifiers
  • wos:000246120700017
  • scopus:34247874564
ISSN
1535-9778
DOI
10.1128/EC.00338-06
language
English
LU publication?
yes
id
a979803e-197c-4f09-a06f-53a2c9df8150 (old id 663138)
date added to LUP
2007-12-21 13:08:13
date last changed
2017-10-01 03:44:48
@article{a979803e-197c-4f09-a06f-53a2c9df8150,
  abstract     = {The hemiascomycete yeast Dekkera bruxellensis, also known as Brettanomyces bruxellensis, is a major cause of wine spoilage worldwide. Wines infected with D. bruxellensis develop distinctive, unpleasant aromas due to volatile phenols produced by this species, which is highly ethanol tolerant and facultatively anaerobic. Despite its importance, however, D. bruxellensis has been poorly genetically characterized until now. We performed genome survey sequencing of a wine strain of D. bruxellensis to obtain 0.4x coverage of the genome. We identified approximately 3,000 genes, whose products averaged 49% amino acid identity to their Saccharomyces cerevisiae orthologs, with similar intron contents. Maximum likelihood phylogenetic analyses suggest that the relationship between D. brurellensis, S. cerevisiae, and Candida albicans is close to a trichotomy. The estimated rate of chromosomal rearrangement in D. bruxellensis is slower than that calculated for C albicans, while its rate of amino acid evolution is somewhat higher. The proteome of D. bruxellensis is enriched for transporters and genes involved in nitrogen and lipid metabolism, among other functions, which may reflect adaptations to its low-nutrient, high-ethanol niche. We also identified an adenyl deaminase gene that has high similarity to a gene in bacteria of the Burkholderia cepacia species complex and appears to be the result of horizontal gene transfer. These data provide a resource for further analyses of the population genetics and evolution of D. bruxellensis and of the genetic bases of its physiological capabilities.},
  author       = {Woolfit, Megan and Rozpedowska, Elzbieta and Piskur, Jure and Wolfe, Kenneth H.},
  issn         = {1535-9778},
  language     = {eng},
  number       = {4},
  pages        = {721--733},
  publisher    = {American Society for Microbiology},
  series       = {Eukaryotic Cell},
  title        = {Genome survey sequencing of the wine spoilage yeast Dekkera (Brettanomyces) bruxellensis},
  url          = {http://dx.doi.org/10.1128/EC.00338-06},
  volume       = {6},
  year         = {2007},
}