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Magnitude of rise in proneurotensin is related to amount of triglyceride appearance in blood after standardized oral intake of both saturated and unsaturated fat

Fawad, Ayesha LU ; Fernandez, Celine LU ; Bergmann, Andreas ; Struck, Joachim ; Nilsson, Peter M. LU ; Bennet, Louise LU orcid ; Orho-Melander, Marju LU and Melander, Olle LU orcid (2020) In Lipids in Health and Disease 19(1).
Abstract

BACKGROUND: In rodents, neurotensin contributes to high fat diet induced obesity by facilitation of intestinal fat absorption. The effect of oral lipid load on plasma proneurotensin and relationship with plasma triglycerides in humans is unknown. AIM: To investigate the acute effects of an oral lipid load on proneurotensin and plasma triglycerides and their interrelationships in healthy individuals. SETTING/ METHODS: Twenty-two healthy subjects were given 150 mL of full milk cream (54 g fat) and 59 mL of pure olive oil (54 g fat) in the fasted state at two different occasions separated by at least 1 week in random order. Venous blood was drawn at fasted before 0 h (h) and at 1 h, 2 h and 4 h after ingestion. Post-ingested values of... (More)

BACKGROUND: In rodents, neurotensin contributes to high fat diet induced obesity by facilitation of intestinal fat absorption. The effect of oral lipid load on plasma proneurotensin and relationship with plasma triglycerides in humans is unknown. AIM: To investigate the acute effects of an oral lipid load on proneurotensin and plasma triglycerides and their interrelationships in healthy individuals. SETTING/ METHODS: Twenty-two healthy subjects were given 150 mL of full milk cream (54 g fat) and 59 mL of pure olive oil (54 g fat) in the fasted state at two different occasions separated by at least 1 week in random order. Venous blood was drawn at fasted before 0 h (h) and at 1 h, 2 h and 4 h after ingestion. Post-ingested values of proneurotensin and plasma triglycerides were compared with fasting levels and post ingestion Area Under the Curve (AUC) of proneurotensin was correlated with that of plasma triglycerides. RESULTS: An immediate rise of plasma proneurotensin and plasma triglycerides were observed after ingestion of cream with maximum increase at 2 h for proneurotensin [mean (95% confidence interval)] of 22 (12-31) pmol/L (P < 0.001) and at 3 h for triglycerides of 0.60 (0.43-0.78) mmol/L (P < 0.001). Similarly, plasma proneurotensin and plasma triglycerides increased after ingestion of olive oil with maximum increase of proneurotensin at 3 h of 62 (46-78) pmol/L (P < 0.001) and plasma triglycerides at 3 h of 0.32 (0.18-0.45) mmol/L (P < 0.001). The post lipid load AUC for proneurotensin correlated significantly with the AUC for plasma triglycerides both after cream (r = 0.49, P = 0.021) and olive oil (r = 0.55, P = 0.008), respectively. CONCLUSION: Proneurotensin increases after an oral lipid load of both cream and olive oil and the rise of post-ingestion plasma triglycerides is significantly related to the rise of post-ingestion proneurotensin.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Fat absorption, Gut hormones, Obesity, Proneurotensin, Triglycerides, Type 2 diabetes
in
Lipids in Health and Disease
volume
19
issue
1
article number
191
publisher
BioMed Central (BMC)
external identifiers
  • pmid:32825823
  • scopus:85089794723
ISSN
1476-511X
DOI
10.1186/s12944-020-01361-0
project
AIR Lund - Artificially Intelligent use of Registers
language
English
LU publication?
yes
id
663c2678-1238-4720-bc76-a94a10b9c142
date added to LUP
2020-09-07 12:37:34
date last changed
2024-05-29 19:18:41
@article{663c2678-1238-4720-bc76-a94a10b9c142,
  abstract     = {{<p>BACKGROUND: In rodents, neurotensin contributes to high fat diet induced obesity by facilitation of intestinal fat absorption. The effect of oral lipid load on plasma proneurotensin and relationship with plasma triglycerides in humans is unknown. AIM: To investigate the acute effects of an oral lipid load on proneurotensin and plasma triglycerides and their interrelationships in healthy individuals. SETTING/ METHODS: Twenty-two healthy subjects were given 150 mL of full milk cream (54 g fat) and 59 mL of pure olive oil (54 g fat) in the fasted state at two different occasions separated by at least 1 week in random order. Venous blood was drawn at fasted before 0 h (h) and at 1 h, 2 h and 4 h after ingestion. Post-ingested values of proneurotensin and plasma triglycerides were compared with fasting levels and post ingestion Area Under the Curve (AUC) of proneurotensin was correlated with that of plasma triglycerides. RESULTS: An immediate rise of plasma proneurotensin and plasma triglycerides were observed after ingestion of cream with maximum increase at 2 h for proneurotensin [mean (95% confidence interval)] of 22 (12-31) pmol/L (P &lt; 0.001) and at 3 h for triglycerides of 0.60 (0.43-0.78) mmol/L (P &lt; 0.001). Similarly, plasma proneurotensin and plasma triglycerides increased after ingestion of olive oil with maximum increase of proneurotensin at 3 h of 62 (46-78) pmol/L (P &lt; 0.001) and plasma triglycerides at 3 h of 0.32 (0.18-0.45) mmol/L (P &lt; 0.001). The post lipid load AUC for proneurotensin correlated significantly with the AUC for plasma triglycerides both after cream (r = 0.49, P = 0.021) and olive oil (r = 0.55, P = 0.008), respectively. CONCLUSION: Proneurotensin increases after an oral lipid load of both cream and olive oil and the rise of post-ingestion plasma triglycerides is significantly related to the rise of post-ingestion proneurotensin.</p>}},
  author       = {{Fawad, Ayesha and Fernandez, Celine and Bergmann, Andreas and Struck, Joachim and Nilsson, Peter M. and Bennet, Louise and Orho-Melander, Marju and Melander, Olle}},
  issn         = {{1476-511X}},
  keywords     = {{Fat absorption; Gut hormones; Obesity; Proneurotensin; Triglycerides; Type 2 diabetes}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{BioMed Central (BMC)}},
  series       = {{Lipids in Health and Disease}},
  title        = {{Magnitude of rise in proneurotensin is related to amount of triglyceride appearance in blood after standardized oral intake of both saturated and unsaturated fat}},
  url          = {{http://dx.doi.org/10.1186/s12944-020-01361-0}},
  doi          = {{10.1186/s12944-020-01361-0}},
  volume       = {{19}},
  year         = {{2020}},
}