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Automated solid-phase extraction and liquid chromatography tandem mass spectrometry determination of N-methyl-2-pyrrolidone and its metabolites in urine and plasma

Cohen, Arieh LU and Jönsson, Bo A LU (2007) In Chromatographia 65(7-8). p.407-412
Abstract
A method for the simultaneous determination of N-methyl-2-pyrrolidone (NMP) and its metabolites 5-hydroxyl-N-pyrrolidone (5HNMP), N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2HMSI) in plasma and urine has been developed. Samples were purified by SPE using an ASPEC XL4. Analysis was performed using LC-MS equipped with an APCI interface. The analysis provided linear responses in the range of 0.125-12 mu g mL(-1) for all of the analytes and up to 150 mu g mL(-1) for 5HNMP and 2HMS1. The within day precision was in the range of 0.9-19.1% for plasma samples and 1.9-10.4% for urine samples whereas the between day precisions were 4.5-11.9% and 1.2-17.5%, respectively. The method was deemed to be suitable for monitoring the levels... (More)
A method for the simultaneous determination of N-methyl-2-pyrrolidone (NMP) and its metabolites 5-hydroxyl-N-pyrrolidone (5HNMP), N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2HMSI) in plasma and urine has been developed. Samples were purified by SPE using an ASPEC XL4. Analysis was performed using LC-MS equipped with an APCI interface. The analysis provided linear responses in the range of 0.125-12 mu g mL(-1) for all of the analytes and up to 150 mu g mL(-1) for 5HNMP and 2HMS1. The within day precision was in the range of 0.9-19.1% for plasma samples and 1.9-10.4% for urine samples whereas the between day precisions were 4.5-11.9% and 1.2-17.5%, respectively. The method was deemed to be suitable for monitoring the levels of NMP and its metabolites in the plasma and urine of occupationally exposed persons. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
column liquid chromatography-mass spectrometry, solid-phase extraction, N-methyl-2-pyrrolidone in Urine and plasma
in
Chromatographia
volume
65
issue
7-8
pages
407 - 412
publisher
Vieweg Verlag
external identifiers
  • wos:000246055800003
  • scopus:34247609805
ISSN
0009-5893
DOI
10.1365/s10337-007-0173-2
language
English
LU publication?
yes
id
c631395b-02cb-4e31-9823-6eb458388b4e (old id 666548)
date added to LUP
2007-12-06 10:51:20
date last changed
2017-10-29 04:14:09
@article{c631395b-02cb-4e31-9823-6eb458388b4e,
  abstract     = {A method for the simultaneous determination of N-methyl-2-pyrrolidone (NMP) and its metabolites 5-hydroxyl-N-pyrrolidone (5HNMP), N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2HMSI) in plasma and urine has been developed. Samples were purified by SPE using an ASPEC XL4. Analysis was performed using LC-MS equipped with an APCI interface. The analysis provided linear responses in the range of 0.125-12 mu g mL(-1) for all of the analytes and up to 150 mu g mL(-1) for 5HNMP and 2HMS1. The within day precision was in the range of 0.9-19.1% for plasma samples and 1.9-10.4% for urine samples whereas the between day precisions were 4.5-11.9% and 1.2-17.5%, respectively. The method was deemed to be suitable for monitoring the levels of NMP and its metabolites in the plasma and urine of occupationally exposed persons.},
  author       = {Cohen, Arieh and Jönsson, Bo A},
  issn         = {0009-5893},
  keyword      = {column liquid chromatography-mass spectrometry,solid-phase extraction,N-methyl-2-pyrrolidone in Urine and plasma},
  language     = {eng},
  number       = {7-8},
  pages        = {407--412},
  publisher    = {Vieweg Verlag},
  series       = {Chromatographia},
  title        = {Automated solid-phase extraction and liquid chromatography tandem mass spectrometry determination of N-methyl-2-pyrrolidone and its metabolites in urine and plasma},
  url          = {http://dx.doi.org/10.1365/s10337-007-0173-2},
  volume       = {65},
  year         = {2007},
}