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A constitutive catabolite repression mutant of a recombinant Saccharomyces cerevisiae strain improves xylose consumption during fermentation

Thanvanthri, Vasu LU ; Gorwa-Grauslund, Marie-Francoise LU ; Hahn-Hägerdal, Bärbel LU ; Pretorius, Isak S. and Otero, Ricardo R. Cordero (2007) In Annals of Microbiology 57(1). p.85-92
Abstract
Efficient xylose utilisation by microorganisms is of importance to the lignocellulose fermentation industry. The aim of this work was to develop constitutive catabolite repression mutants in a xylose-utilising recombinant Saccharomyces cerevisiae strain and evaluate the differences in xylose consumption under fermentation conditions. S. cerevisiae YUSM was constitutively catabolite repressed through specific disruptions within the MIG1 gene. The strains were grown aerobically in synthetic complete medium with xylose as the sole carbon source. Constitutive catabolite repressed strain YCR17 grew four-fold better on xylose in aerobic conditions than the control strain YUSM. Anaerobic batch fermentation in minimal medium with glucose-xylose... (More)
Efficient xylose utilisation by microorganisms is of importance to the lignocellulose fermentation industry. The aim of this work was to develop constitutive catabolite repression mutants in a xylose-utilising recombinant Saccharomyces cerevisiae strain and evaluate the differences in xylose consumption under fermentation conditions. S. cerevisiae YUSM was constitutively catabolite repressed through specific disruptions within the MIG1 gene. The strains were grown aerobically in synthetic complete medium with xylose as the sole carbon source. Constitutive catabolite repressed strain YCR17 grew four-fold better on xylose in aerobic conditions than the control strain YUSM. Anaerobic batch fermentation in minimal medium with glucose-xylose mixtures and N-limited chemostats with varying sugar concentrations were performed. Sugar utilisation and metabolite production during fermentation were monitored. YCR17 exhibited a faster xylose consumption rate than YUSM under high glucose conditions in nitrogen-limited chemostat cultivations. This study shows that a constitutive catabolite repressed mutant could be used to enhance the xylose consumption rate even in the presence of high glucose in the fermentation medium. This could help in reducing fermentation time and cost in mixed sugar fermentation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
constitutive catabolite repression, MIG1, xylose fermentation, xylitol, Saccharomyces cerevisiae
in
Annals of Microbiology
volume
57
issue
1
pages
85 - 92
publisher
INST MICROBIOLOGIA
external identifiers
  • wos:000245462200014
  • scopus:34147110457
ISSN
1590-4261
language
English
LU publication?
yes
id
aeb6fa18-23f3-40d8-806b-e0e28319a908 (old id 668023)
alternative location
http://www.annmicro.unimi.it/abst/57/14%20Thanvanthri%20Gururajan_85-92.pdf
date added to LUP
2007-12-11 11:15:05
date last changed
2017-01-01 05:02:45
@article{aeb6fa18-23f3-40d8-806b-e0e28319a908,
  abstract     = {Efficient xylose utilisation by microorganisms is of importance to the lignocellulose fermentation industry. The aim of this work was to develop constitutive catabolite repression mutants in a xylose-utilising recombinant Saccharomyces cerevisiae strain and evaluate the differences in xylose consumption under fermentation conditions. S. cerevisiae YUSM was constitutively catabolite repressed through specific disruptions within the MIG1 gene. The strains were grown aerobically in synthetic complete medium with xylose as the sole carbon source. Constitutive catabolite repressed strain YCR17 grew four-fold better on xylose in aerobic conditions than the control strain YUSM. Anaerobic batch fermentation in minimal medium with glucose-xylose mixtures and N-limited chemostats with varying sugar concentrations were performed. Sugar utilisation and metabolite production during fermentation were monitored. YCR17 exhibited a faster xylose consumption rate than YUSM under high glucose conditions in nitrogen-limited chemostat cultivations. This study shows that a constitutive catabolite repressed mutant could be used to enhance the xylose consumption rate even in the presence of high glucose in the fermentation medium. This could help in reducing fermentation time and cost in mixed sugar fermentation.},
  author       = {Thanvanthri, Vasu and Gorwa-Grauslund, Marie-Francoise and Hahn-Hägerdal, Bärbel and Pretorius, Isak S. and Otero, Ricardo R. Cordero},
  issn         = {1590-4261},
  keyword      = {constitutive catabolite repression,MIG1,xylose fermentation,xylitol,Saccharomyces cerevisiae},
  language     = {eng},
  number       = {1},
  pages        = {85--92},
  publisher    = {INST MICROBIOLOGIA},
  series       = {Annals of Microbiology},
  title        = {A constitutive catabolite repression mutant of a recombinant Saccharomyces cerevisiae strain improves xylose consumption during fermentation},
  volume       = {57},
  year         = {2007},
}