A constitutive catabolite repression mutant of a recombinant Saccharomyces cerevisiae strain improves xylose consumption during fermentation
(2007) In Annals of Microbiology 57(1). p.85-92- Abstract
- Efficient xylose utilisation by microorganisms is of importance to the lignocellulose fermentation industry. The aim of this work was to develop constitutive catabolite repression mutants in a xylose-utilising recombinant Saccharomyces cerevisiae strain and evaluate the differences in xylose consumption under fermentation conditions. S. cerevisiae YUSM was constitutively catabolite repressed through specific disruptions within the MIG1 gene. The strains were grown aerobically in synthetic complete medium with xylose as the sole carbon source. Constitutive catabolite repressed strain YCR17 grew four-fold better on xylose in aerobic conditions than the control strain YUSM. Anaerobic batch fermentation in minimal medium with glucose-xylose... (More)
- Efficient xylose utilisation by microorganisms is of importance to the lignocellulose fermentation industry. The aim of this work was to develop constitutive catabolite repression mutants in a xylose-utilising recombinant Saccharomyces cerevisiae strain and evaluate the differences in xylose consumption under fermentation conditions. S. cerevisiae YUSM was constitutively catabolite repressed through specific disruptions within the MIG1 gene. The strains were grown aerobically in synthetic complete medium with xylose as the sole carbon source. Constitutive catabolite repressed strain YCR17 grew four-fold better on xylose in aerobic conditions than the control strain YUSM. Anaerobic batch fermentation in minimal medium with glucose-xylose mixtures and N-limited chemostats with varying sugar concentrations were performed. Sugar utilisation and metabolite production during fermentation were monitored. YCR17 exhibited a faster xylose consumption rate than YUSM under high glucose conditions in nitrogen-limited chemostat cultivations. This study shows that a constitutive catabolite repressed mutant could be used to enhance the xylose consumption rate even in the presence of high glucose in the fermentation medium. This could help in reducing fermentation time and cost in mixed sugar fermentation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/668023
- author
- Thanvanthri, Vasu LU ; Gorwa-Grauslund, Marie-Francoise LU ; Hahn-Hägerdal, Bärbel LU ; Pretorius, Isak S. and Otero, Ricardo R. Cordero
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- constitutive catabolite repression, MIG1, xylose fermentation, xylitol, Saccharomyces cerevisiae
- in
- Annals of Microbiology
- volume
- 57
- issue
- 1
- pages
- 85 - 92
- publisher
- Springer
- external identifiers
-
- wos:000245462200014
- scopus:34147110457
- ISSN
- 1590-4261
- language
- English
- LU publication?
- yes
- id
- aeb6fa18-23f3-40d8-806b-e0e28319a908 (old id 668023)
- alternative location
- http://www.annmicro.unimi.it/abst/57/14%20Thanvanthri%20Gururajan_85-92.pdf
- date added to LUP
- 2016-04-01 12:19:38
- date last changed
- 2022-01-27 02:07:15
@article{aeb6fa18-23f3-40d8-806b-e0e28319a908,
abstract = {{Efficient xylose utilisation by microorganisms is of importance to the lignocellulose fermentation industry. The aim of this work was to develop constitutive catabolite repression mutants in a xylose-utilising recombinant Saccharomyces cerevisiae strain and evaluate the differences in xylose consumption under fermentation conditions. S. cerevisiae YUSM was constitutively catabolite repressed through specific disruptions within the MIG1 gene. The strains were grown aerobically in synthetic complete medium with xylose as the sole carbon source. Constitutive catabolite repressed strain YCR17 grew four-fold better on xylose in aerobic conditions than the control strain YUSM. Anaerobic batch fermentation in minimal medium with glucose-xylose mixtures and N-limited chemostats with varying sugar concentrations were performed. Sugar utilisation and metabolite production during fermentation were monitored. YCR17 exhibited a faster xylose consumption rate than YUSM under high glucose conditions in nitrogen-limited chemostat cultivations. This study shows that a constitutive catabolite repressed mutant could be used to enhance the xylose consumption rate even in the presence of high glucose in the fermentation medium. This could help in reducing fermentation time and cost in mixed sugar fermentation.}},
author = {{Thanvanthri, Vasu and Gorwa-Grauslund, Marie-Francoise and Hahn-Hägerdal, Bärbel and Pretorius, Isak S. and Otero, Ricardo R. Cordero}},
issn = {{1590-4261}},
keywords = {{constitutive catabolite repression; MIG1; xylose fermentation; xylitol; Saccharomyces cerevisiae}},
language = {{eng}},
number = {{1}},
pages = {{85--92}},
publisher = {{Springer}},
series = {{Annals of Microbiology}},
title = {{A constitutive catabolite repression mutant of a recombinant Saccharomyces cerevisiae strain improves xylose consumption during fermentation}},
url = {{http://www.annmicro.unimi.it/abst/57/14%20Thanvanthri%20Gururajan_85-92.pdf}},
volume = {{57}},
year = {{2007}},
}