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Reversed phase continuous full filling CEC-ESI-MS

Viberg, Peter LU ; Spégel, Peter LU ; Nilsson, J.; Petersson, P.; Joernten-Karlsson, M. and Nilsson, S. (2007) In Chromatographia 65(5-6). p.291-297
Abstract
Nanoparticles were used as a pseudostationary phase in capillary electrochromatography (CEC) electrospray ionisation-mass spectrometry (ESI-MS) for separation of both neutral analytes by a reversed phase mechanism, as well as for cationic analytes by a cation exchange mechanism. Nanoparticles suspended in electrolyte, were injected as a plug prior to the sample using a partial filling technique (PF), or used as electrolyte in a continuous full filling (CFF) technique. An orthogonal ESI probe was used to hinder the nanoparticles from entering the mass spectrometer and to allow detection of analytes co-eluting with concentrated nanoparticle slurries. Two types of nanoparticles were synthesised and used, both of them having a hydrophobic core... (More)
Nanoparticles were used as a pseudostationary phase in capillary electrochromatography (CEC) electrospray ionisation-mass spectrometry (ESI-MS) for separation of both neutral analytes by a reversed phase mechanism, as well as for cationic analytes by a cation exchange mechanism. Nanoparticles suspended in electrolyte, were injected as a plug prior to the sample using a partial filling technique (PF), or used as electrolyte in a continuous full filling (CFF) technique. An orthogonal ESI probe was used to hinder the nanoparticles from entering the mass spectrometer and to allow detection of analytes co-eluting with concentrated nanoparticle slurries. Two types of nanoparticles were synthesised and used, both of them having a hydrophobic core and a hydrophilic surface. The hydrophobic core gave the nanoparticles reversed phase properties and the hydrophilic surface promoted the formation of stable slurries of nanoparticles in electrolytes with a low concentration of organic modifier. The surface of one of the nanoparticle types was covered with sulphate groups that, besides from enhancing slurry stability and thus enabling reversed phase CEC, also enabled ion exchange CEC. Both nanoparticle types showed reproducible and interpretable retention properties. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
pseudostationary phase, nanoparticles, spectrometry, capillary electrochromatography, electrospray ionisation-mass, partial filling, continuous full filling
in
Chromatographia
volume
65
issue
5-6
pages
291 - 297
publisher
Vieweg Verlag
external identifiers
  • wos:000245397900005
  • scopus:33847613376
ISSN
0009-5893
DOI
10.1365/s10337-006-0165-7
language
English
LU publication?
yes
id
c3f69179-f6a3-4bef-9c15-4de68c9b8c01 (old id 669858)
date added to LUP
2008-01-02 16:17:17
date last changed
2017-01-01 07:05:37
@article{c3f69179-f6a3-4bef-9c15-4de68c9b8c01,
  abstract     = {Nanoparticles were used as a pseudostationary phase in capillary electrochromatography (CEC) electrospray ionisation-mass spectrometry (ESI-MS) for separation of both neutral analytes by a reversed phase mechanism, as well as for cationic analytes by a cation exchange mechanism. Nanoparticles suspended in electrolyte, were injected as a plug prior to the sample using a partial filling technique (PF), or used as electrolyte in a continuous full filling (CFF) technique. An orthogonal ESI probe was used to hinder the nanoparticles from entering the mass spectrometer and to allow detection of analytes co-eluting with concentrated nanoparticle slurries. Two types of nanoparticles were synthesised and used, both of them having a hydrophobic core and a hydrophilic surface. The hydrophobic core gave the nanoparticles reversed phase properties and the hydrophilic surface promoted the formation of stable slurries of nanoparticles in electrolytes with a low concentration of organic modifier. The surface of one of the nanoparticle types was covered with sulphate groups that, besides from enhancing slurry stability and thus enabling reversed phase CEC, also enabled ion exchange CEC. Both nanoparticle types showed reproducible and interpretable retention properties.},
  author       = {Viberg, Peter and Spégel, Peter and Nilsson, J. and Petersson, P. and Joernten-Karlsson, M. and Nilsson, S.},
  issn         = {0009-5893},
  keyword      = {pseudostationary phase,nanoparticles,spectrometry,capillary electrochromatography,electrospray ionisation-mass,partial filling,continuous full filling},
  language     = {eng},
  number       = {5-6},
  pages        = {291--297},
  publisher    = {Vieweg Verlag},
  series       = {Chromatographia},
  title        = {Reversed phase continuous full filling CEC-ESI-MS},
  url          = {http://dx.doi.org/10.1365/s10337-006-0165-7},
  volume       = {65},
  year         = {2007},
}