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Tyrosine hydroxylase expression is unstable in a human immortalized mesencephalic cell line - Studies in vitro and after intracerebral grafting in vivo

Paul-Visse, Gesine LU ; Christophersen, Nicolaj LU ; Raymon, Heather; Kiaer, Caroline LU ; Smith, Ruben LU and Brundin, Patrik LU (2007) In Molecular and Cellular Neuroscience 34(3). p.390-399
Abstract
We have studied the stability of the dopaminergic phenotype in a conditionally immortalized human mesencephalic cell line, NIESC2.10. Even though MESC2.10 cells exhibit features of dopaminergic neurons in vitro, none of the cells expressed tyrosine hydroxylase (TH) after transplantation into a rat model of Parkinson's disease. We examined whether this is caused by cell death or loss of transmitter phenotype. Cells were cultured in differentiation medium, then harvested and replated into the same medium where they continued to express TH, whereas replated cells fed medium lacking differentiation factors (dibutyryl cAMP and glial cell line-derived neurotrophic factor) did not. Interestingly, cultures grown in the absence of differentiation... (More)
We have studied the stability of the dopaminergic phenotype in a conditionally immortalized human mesencephalic cell line, NIESC2.10. Even though MESC2.10 cells exhibit features of dopaminergic neurons in vitro, none of the cells expressed tyrosine hydroxylase (TH) after transplantation into a rat model of Parkinson's disease. We examined whether this is caused by cell death or loss of transmitter phenotype. Cells were cultured in differentiation medium, then harvested and replated into the same medium where they continued to express TH, whereas replated cells fed medium lacking differentiation factors (dibutyryl cAMP and glial cell line-derived neurotrophic factor) did not. Interestingly, cultures grown in the absence of differentiation factors could regain TH expression once exposed to differentiation medium. Our data suggest that TH expression in vitro is inducible in neurons derived from the NIESC2.10 cell line and that the dopaminergic phenotype of these cells in vivo might be unstable. (c) 2006 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular and Cellular Neuroscience
volume
34
issue
3
pages
390 - 399
publisher
Elsevier
external identifiers
  • wos:000244909200010
  • scopus:33847314150
ISSN
1044-7431
DOI
10.1016/j.mcn.2006.11.010
language
English
LU publication?
yes
id
7621f7d0-e684-42df-a0e2-6991790efd10 (old id 670035)
date added to LUP
2007-12-14 16:03:06
date last changed
2017-09-24 03:39:55
@article{7621f7d0-e684-42df-a0e2-6991790efd10,
  abstract     = {We have studied the stability of the dopaminergic phenotype in a conditionally immortalized human mesencephalic cell line, NIESC2.10. Even though MESC2.10 cells exhibit features of dopaminergic neurons in vitro, none of the cells expressed tyrosine hydroxylase (TH) after transplantation into a rat model of Parkinson's disease. We examined whether this is caused by cell death or loss of transmitter phenotype. Cells were cultured in differentiation medium, then harvested and replated into the same medium where they continued to express TH, whereas replated cells fed medium lacking differentiation factors (dibutyryl cAMP and glial cell line-derived neurotrophic factor) did not. Interestingly, cultures grown in the absence of differentiation factors could regain TH expression once exposed to differentiation medium. Our data suggest that TH expression in vitro is inducible in neurons derived from the NIESC2.10 cell line and that the dopaminergic phenotype of these cells in vivo might be unstable. (c) 2006 Elsevier Inc. All rights reserved.},
  author       = {Paul-Visse, Gesine and Christophersen, Nicolaj and Raymon, Heather and Kiaer, Caroline and Smith, Ruben and Brundin, Patrik},
  issn         = {1044-7431},
  language     = {eng},
  number       = {3},
  pages        = {390--399},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Neuroscience},
  title        = {Tyrosine hydroxylase expression is unstable in a human immortalized mesencephalic cell line - Studies in vitro and after intracerebral grafting in vivo},
  url          = {http://dx.doi.org/10.1016/j.mcn.2006.11.010},
  volume       = {34},
  year         = {2007},
}