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A new standardized lipolysis approach for characterization of emulsions and dispersions

Brogard, M.; Troedsson, E; Thuresson, Krister LU and Ljusberg, Helena LU (2007) In Journal of Colloid and Interface Science 308(2). p.500-507
Abstract
A new standardized lipolysis approach is presented where the focus is on the initial rate of lipolysis. An advantage is that data obtained in this way reflect degradation before growing amounts of lipolysis products retard the process. The method can be used to rank different lipase substrates. In particular, the method can be used to obtain information about the susceptibility to degradation of various emulsions and dispersions that are used in technical applications. We present how the method is standardized to facilitate comparison of various substrates. This involves (i) lipase substrate in excess, i.e., the amount of lipase is rate limiting, and (ii) expressing rate of degradation relative to that of a reference substrate, tributyrin.... (More)
A new standardized lipolysis approach is presented where the focus is on the initial rate of lipolysis. An advantage is that data obtained in this way reflect degradation before growing amounts of lipolysis products retard the process. The method can be used to rank different lipase substrates. In particular, the method can be used to obtain information about the susceptibility to degradation of various emulsions and dispersions that are used in technical applications. We present how the method is standardized to facilitate comparison of various substrates. This involves (i) lipase substrate in excess, i.e., the amount of lipase is rate limiting, and (ii) expressing rate of degradation relative to that of a reference substrate, tributyrin. Under such conditions, with the amount of lipase substrate held constant, an increase in enzymatic activity will generate a proportional increase in the lipolysis rate. This enables comparison of results obtained from different enzyme batches and corrects for day-to-day variability. Examples illustrating the potential of the method to discriminate and rank different lipase substrates with regard to enzymatic degradation are presented. (c) 2006 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
self-emulsifying drug delivery systems (SEDDS), lipolysis, digestion, lipid, lipid-based drug delivery, structured lipids, lamellar, dispersion, triglyceride, emulsion
in
Journal of Colloid and Interface Science
volume
308
issue
2
pages
500 - 507
publisher
Elsevier
external identifiers
  • wos:000244841500027
  • scopus:33847260132
ISSN
1095-7103
DOI
10.1016/j.jcis.2006.12.015
language
English
LU publication?
yes
id
ddd971cc-6762-4a50-92fe-befb3f150290 (old id 670429)
date added to LUP
2007-12-11 16:04:31
date last changed
2017-01-01 05:07:26
@article{ddd971cc-6762-4a50-92fe-befb3f150290,
  abstract     = {A new standardized lipolysis approach is presented where the focus is on the initial rate of lipolysis. An advantage is that data obtained in this way reflect degradation before growing amounts of lipolysis products retard the process. The method can be used to rank different lipase substrates. In particular, the method can be used to obtain information about the susceptibility to degradation of various emulsions and dispersions that are used in technical applications. We present how the method is standardized to facilitate comparison of various substrates. This involves (i) lipase substrate in excess, i.e., the amount of lipase is rate limiting, and (ii) expressing rate of degradation relative to that of a reference substrate, tributyrin. Under such conditions, with the amount of lipase substrate held constant, an increase in enzymatic activity will generate a proportional increase in the lipolysis rate. This enables comparison of results obtained from different enzyme batches and corrects for day-to-day variability. Examples illustrating the potential of the method to discriminate and rank different lipase substrates with regard to enzymatic degradation are presented. (c) 2006 Elsevier Inc. All rights reserved.},
  author       = {Brogard, M. and Troedsson, E and Thuresson, Krister and Ljusberg, Helena},
  issn         = {1095-7103},
  keyword      = {self-emulsifying drug delivery systems (SEDDS),lipolysis,digestion,lipid,lipid-based drug delivery,structured lipids,lamellar,dispersion,triglyceride,emulsion},
  language     = {eng},
  number       = {2},
  pages        = {500--507},
  publisher    = {Elsevier},
  series       = {Journal of Colloid and Interface Science},
  title        = {A new standardized lipolysis approach for characterization of emulsions and dispersions},
  url          = {http://dx.doi.org/10.1016/j.jcis.2006.12.015},
  volume       = {308},
  year         = {2007},
}