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Minimizing tissue-material interaction in microsensor for subcutaneous glucose monitoring

Ahmad, Farook; Christenson, Andreas LU ; Bainbridge, Martina; Yusof, Ahmad Pauzi Mohd and Ab Ghani, Sulaiman (2007) In Biosensors & Bioelectronics 22(8). p.1625-1632
Abstract
A new implantable electrocatalytic glucose sensor for subcutaneous glucose monitoring has been fabricated by immobilizing glucose oxidase on a chemically modified carbon fiber. The sensor was inserted subcutaneously on a male spraguely rat without any incision after dipping the microsensor in the rat's serum for 3 days. The so called "stained" microsensor, operated in the amperometric mode with an applied potential of +0.23 V versus Ag vertical bar AgCl, was able to directly measure the glucose concentration upon infusion of glucose. The results obtained were encouraging, with the response time was less than 2 s and the apparent Michaelis-Menten value at 5.1 +/- 0.5 mM. The "stained" microsensor shows good stability and reproducibility... (More)
A new implantable electrocatalytic glucose sensor for subcutaneous glucose monitoring has been fabricated by immobilizing glucose oxidase on a chemically modified carbon fiber. The sensor was inserted subcutaneously on a male spraguely rat without any incision after dipping the microsensor in the rat's serum for 3 days. The so called "stained" microsensor, operated in the amperometric mode with an applied potential of +0.23 V versus Ag vertical bar AgCl, was able to directly measure the glucose concentration upon infusion of glucose. The results obtained were encouraging, with the response time was less than 2 s and the apparent Michaelis-Menten value at 5.1 +/- 0.5 mM. The "stained" microsensor shows good stability and reproducibility with constant response spanned over 25 days. Most common interferences in glucose analysis were minimized by the outerlayer Nafion((R)). Hematology examinations showed minimal material-tissue interaction. Use of such mechanical devices will allow a more refined understanding towards glucose control in diabetic patients as the implanted microsensor was not effected by biocompatibility failures. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
biocompatibility, hematology, subcutaneous, microsensor, rat's serum
in
Biosensors & Bioelectronics
volume
22
issue
8
pages
1625 - 1632
publisher
Elsevier
external identifiers
  • wos:000244676300009
  • scopus:33846807467
ISSN
1873-4235
DOI
10.1016/j.bios.2006.07.025
language
English
LU publication?
yes
id
715ac82b-c989-49d6-a69c-57d47ff2c173 (old id 671790)
date added to LUP
2007-12-11 12:57:40
date last changed
2017-01-01 06:52:28
@article{715ac82b-c989-49d6-a69c-57d47ff2c173,
  abstract     = {A new implantable electrocatalytic glucose sensor for subcutaneous glucose monitoring has been fabricated by immobilizing glucose oxidase on a chemically modified carbon fiber. The sensor was inserted subcutaneously on a male spraguely rat without any incision after dipping the microsensor in the rat's serum for 3 days. The so called "stained" microsensor, operated in the amperometric mode with an applied potential of +0.23 V versus Ag vertical bar AgCl, was able to directly measure the glucose concentration upon infusion of glucose. The results obtained were encouraging, with the response time was less than 2 s and the apparent Michaelis-Menten value at 5.1 +/- 0.5 mM. The "stained" microsensor shows good stability and reproducibility with constant response spanned over 25 days. Most common interferences in glucose analysis were minimized by the outerlayer Nafion((R)). Hematology examinations showed minimal material-tissue interaction. Use of such mechanical devices will allow a more refined understanding towards glucose control in diabetic patients as the implanted microsensor was not effected by biocompatibility failures. (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Ahmad, Farook and Christenson, Andreas and Bainbridge, Martina and Yusof, Ahmad Pauzi Mohd and Ab Ghani, Sulaiman},
  issn         = {1873-4235},
  keyword      = {biocompatibility,hematology,subcutaneous,microsensor,rat's serum},
  language     = {eng},
  number       = {8},
  pages        = {1625--1632},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Minimizing tissue-material interaction in microsensor for subcutaneous glucose monitoring},
  url          = {http://dx.doi.org/10.1016/j.bios.2006.07.025},
  volume       = {22},
  year         = {2007},
}