Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

The use of single walled carbon nanotubes dispersed in a chitosan matrix for preparation of a galactose biosensor

Tkac, Jan LU ; Whittaker, James W. and Ruzgas, Tautgirdas LU (2007) In Biosensors & Bioelectronics 22(8). p.1820-1824
Abstract
Chitosan was chosen as a natural polymer for dispersion of single walled carbon nanotubes (SWNT) based on its ability to efficiently solubilize SWNTs to form a stable dispersion. Moreover, chitosan films deposited on a surface of a glassy carbon (GC) electrode are mechanically stable. Further stabilisation of the chitosan film containing SWNT (CHIT-SWNT) was done by chemical crosslinking with glutaraldehyde and free aldehyde groups produced a substrate used for covalent immobilisation of galactose oxidase (GalOD). Different galactose biosensor configurations were tested with optimisation of composition of inner and outer membrane; and enzyme immobilisation procedure, as well. Detection of oxygen uptake by GalOD on CHIT-SWNT layer at -400... (More)
Chitosan was chosen as a natural polymer for dispersion of single walled carbon nanotubes (SWNT) based on its ability to efficiently solubilize SWNTs to form a stable dispersion. Moreover, chitosan films deposited on a surface of a glassy carbon (GC) electrode are mechanically stable. Further stabilisation of the chitosan film containing SWNT (CHIT-SWNT) was done by chemical crosslinking with glutaraldehyde and free aldehyde groups produced a substrate used for covalent immobilisation of galactose oxidase (GalOD). Different galactose biosensor configurations were tested with optimisation of composition of inner and outer membrane; and enzyme immobilisation procedure, as well. Detection of oxygen uptake by GalOD on CHIT-SWNT layer at -400 mV is robust and, when flow injection analysis (FIA) was applied for assays, a low detection limit (25 mu M) and very high assay throughput rate (150 h(-1)) was achieved. This new galactose biosensor offers highly reliable detection of galactose with R.S.D. well below 2% and it has been successfully applied to assaying galactose in a blood sample with recovery index between 101.2 and 102.7%. (Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
galactose oxidase, chitosan, carbon nanotube, nation, interferents, blood plasma
in
Biosensors & Bioelectronics
volume
22
issue
8
pages
1820 - 1824
publisher
Elsevier
external identifiers
  • wos:000244676300036
  • scopus:33846820187
ISSN
1873-4235
DOI
10.1016/j.bios.2006.08.014
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
80226366-bb23-4539-a73a-5b22ffc42cbe (old id 671796)
date added to LUP
2016-04-01 15:26:11
date last changed
2022-04-22 07:42:10
@article{80226366-bb23-4539-a73a-5b22ffc42cbe,
  abstract     = {{Chitosan was chosen as a natural polymer for dispersion of single walled carbon nanotubes (SWNT) based on its ability to efficiently solubilize SWNTs to form a stable dispersion. Moreover, chitosan films deposited on a surface of a glassy carbon (GC) electrode are mechanically stable. Further stabilisation of the chitosan film containing SWNT (CHIT-SWNT) was done by chemical crosslinking with glutaraldehyde and free aldehyde groups produced a substrate used for covalent immobilisation of galactose oxidase (GalOD). Different galactose biosensor configurations were tested with optimisation of composition of inner and outer membrane; and enzyme immobilisation procedure, as well. Detection of oxygen uptake by GalOD on CHIT-SWNT layer at -400 mV is robust and, when flow injection analysis (FIA) was applied for assays, a low detection limit (25 mu M) and very high assay throughput rate (150 h(-1)) was achieved. This new galactose biosensor offers highly reliable detection of galactose with R.S.D. well below 2% and it has been successfully applied to assaying galactose in a blood sample with recovery index between 101.2 and 102.7%.}},
  author       = {{Tkac, Jan and Whittaker, James W. and Ruzgas, Tautgirdas}},
  issn         = {{1873-4235}},
  keywords     = {{galactose oxidase; chitosan; carbon nanotube; nation; interferents; blood plasma}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{1820--1824}},
  publisher    = {{Elsevier}},
  series       = {{Biosensors & Bioelectronics}},
  title        = {{The use of single walled carbon nanotubes dispersed in a chitosan matrix for preparation of a galactose biosensor}},
  url          = {{http://dx.doi.org/10.1016/j.bios.2006.08.014}},
  doi          = {{10.1016/j.bios.2006.08.014}},
  volume       = {{22}},
  year         = {{2007}},
}