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The use of single walled carbon nanotubes dispersed in a chitosan matrix for preparation of a galactose biosensor

Tkac, Jan LU ; Whittaker, James W. and Ruzgas, Tautgirdas LU (2007) In Biosensors & Bioelectronics 22(8). p.1820-1824
Abstract
Chitosan was chosen as a natural polymer for dispersion of single walled carbon nanotubes (SWNT) based on its ability to efficiently solubilize SWNTs to form a stable dispersion. Moreover, chitosan films deposited on a surface of a glassy carbon (GC) electrode are mechanically stable. Further stabilisation of the chitosan film containing SWNT (CHIT-SWNT) was done by chemical crosslinking with glutaraldehyde and free aldehyde groups produced a substrate used for covalent immobilisation of galactose oxidase (GalOD). Different galactose biosensor configurations were tested with optimisation of composition of inner and outer membrane; and enzyme immobilisation procedure, as well. Detection of oxygen uptake by GalOD on CHIT-SWNT layer at -400... (More)
Chitosan was chosen as a natural polymer for dispersion of single walled carbon nanotubes (SWNT) based on its ability to efficiently solubilize SWNTs to form a stable dispersion. Moreover, chitosan films deposited on a surface of a glassy carbon (GC) electrode are mechanically stable. Further stabilisation of the chitosan film containing SWNT (CHIT-SWNT) was done by chemical crosslinking with glutaraldehyde and free aldehyde groups produced a substrate used for covalent immobilisation of galactose oxidase (GalOD). Different galactose biosensor configurations were tested with optimisation of composition of inner and outer membrane; and enzyme immobilisation procedure, as well. Detection of oxygen uptake by GalOD on CHIT-SWNT layer at -400 mV is robust and, when flow injection analysis (FIA) was applied for assays, a low detection limit (25 mu M) and very high assay throughput rate (150 h(-1)) was achieved. This new galactose biosensor offers highly reliable detection of galactose with R.S.D. well below 2% and it has been successfully applied to assaying galactose in a blood sample with recovery index between 101.2 and 102.7%. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
galactose oxidase, chitosan, carbon nanotube, nation, interferents, blood plasma
in
Biosensors & Bioelectronics
volume
22
issue
8
pages
1820 - 1824
publisher
Elsevier
external identifiers
  • wos:000244676300036
  • scopus:33846820187
ISSN
1873-4235
DOI
10.1016/j.bios.2006.08.014
language
English
LU publication?
yes
id
80226366-bb23-4539-a73a-5b22ffc42cbe (old id 671796)
date added to LUP
2007-12-19 16:36:21
date last changed
2017-10-01 04:34:30
@article{80226366-bb23-4539-a73a-5b22ffc42cbe,
  abstract     = {Chitosan was chosen as a natural polymer for dispersion of single walled carbon nanotubes (SWNT) based on its ability to efficiently solubilize SWNTs to form a stable dispersion. Moreover, chitosan films deposited on a surface of a glassy carbon (GC) electrode are mechanically stable. Further stabilisation of the chitosan film containing SWNT (CHIT-SWNT) was done by chemical crosslinking with glutaraldehyde and free aldehyde groups produced a substrate used for covalent immobilisation of galactose oxidase (GalOD). Different galactose biosensor configurations were tested with optimisation of composition of inner and outer membrane; and enzyme immobilisation procedure, as well. Detection of oxygen uptake by GalOD on CHIT-SWNT layer at -400 mV is robust and, when flow injection analysis (FIA) was applied for assays, a low detection limit (25 mu M) and very high assay throughput rate (150 h(-1)) was achieved. This new galactose biosensor offers highly reliable detection of galactose with R.S.D. well below 2% and it has been successfully applied to assaying galactose in a blood sample with recovery index between 101.2 and 102.7%.},
  author       = {Tkac, Jan and Whittaker, James W. and Ruzgas, Tautgirdas},
  issn         = {1873-4235},
  keyword      = {galactose oxidase,chitosan,carbon nanotube,nation,interferents,blood plasma},
  language     = {eng},
  number       = {8},
  pages        = {1820--1824},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {The use of single walled carbon nanotubes dispersed in a chitosan matrix for preparation of a galactose biosensor},
  url          = {http://dx.doi.org/10.1016/j.bios.2006.08.014},
  volume       = {22},
  year         = {2007},
}