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Development of reagents for differential protein quantitation by subtractive parent (precursor) ion scanning

Wåhlander, Åsa LU ; Arrigoni, Giorgio LU ; Wårell, Kristofer LU ; Levander, Fredrik LU ; Palmgren, Ronnie; Maloisel, Jean-Luc; Busson, Philippe and James, Peter LU (2007) In Journal of Proteome Research 6(3). p.1101-1113
Abstract
We present a generic approach for quantitative differential proteomics that reduces data complexity in proteome analysis by automated selection of peptides for MS/MS analysis according to their isotope-labeling ratio. Isotopic reagents were developed that give products which fragment easily to generate a unique pair of signature ions. Using the ion-pair ratio, we show that it is possible to select only BSA peptides (with a 3:1 light heavy isotope ratio) for MS/MS when spiked in a whole yeast extract using Parent (precursor) Ion Quantitation Scanning (PIQS) for MS/MS.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
precursor-ion, reagents, mass spectrometry, isotope labeling, HPLC, parent-ion
in
Journal of Proteome Research
volume
6
issue
3
pages
1101 - 1113
publisher
The American Chemical Society
external identifiers
  • wos:000244638400018
  • scopus:33947606962
ISSN
1535-3893
DOI
10.1021/pr0604312
language
English
LU publication?
yes
id
17594dd4-1021-4cd4-9409-d9a2919bf26a (old id 672594)
date added to LUP
2007-12-19 13:47:15
date last changed
2017-01-01 05:13:46
@article{17594dd4-1021-4cd4-9409-d9a2919bf26a,
  abstract     = {We present a generic approach for quantitative differential proteomics that reduces data complexity in proteome analysis by automated selection of peptides for MS/MS analysis according to their isotope-labeling ratio. Isotopic reagents were developed that give products which fragment easily to generate a unique pair of signature ions. Using the ion-pair ratio, we show that it is possible to select only BSA peptides (with a 3:1 light heavy isotope ratio) for MS/MS when spiked in a whole yeast extract using Parent (precursor) Ion Quantitation Scanning (PIQS) for MS/MS.},
  author       = {Wåhlander, Åsa and Arrigoni, Giorgio and Wårell, Kristofer and Levander, Fredrik and Palmgren, Ronnie and Maloisel, Jean-Luc and Busson, Philippe and James, Peter},
  issn         = {1535-3893},
  keyword      = {precursor-ion,reagents,mass spectrometry,isotope labeling,HPLC,parent-ion},
  language     = {eng},
  number       = {3},
  pages        = {1101--1113},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {Development of reagents for differential protein quantitation by subtractive parent (precursor) ion scanning},
  url          = {http://dx.doi.org/10.1021/pr0604312},
  volume       = {6},
  year         = {2007},
}