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On-chip determination of dopamine exocytosis using mercaptopropionic acid modified microelectrodes

Spegel, Christer LU ; Heiskanen, Arto LU ; Acklid, Jenny; Wolff, Anders; Taboryski, Rafael; Emnéus, Jenny LU and Ruzgas, Tautgirdas LU (2007) In Electroanalysis 19(2-3). p.263-271
Abstract
Gold and platinum, which often are used for thin film metallization, are not suitable for the measurement of dopamine (DA), since the oxidation product of DA forms a non-conducting polymer on the electrode surface. In this work several thiols were screened for their ability to prevent this polymerization. It was found that mercaptopropionic acid (MPA) decreased the rate of DA polymerization. MPA, possessing a weak acidic functionality, had the greatest effect on the DA electrochemistry by decreasing electrode passivation, as well as improving reversibility and sensitivity. Modifications of microchip electrodes with MPA did not only improve DA electrochemistry but also significantly increased the storage stability of the transducers. The... (More)
Gold and platinum, which often are used for thin film metallization, are not suitable for the measurement of dopamine (DA), since the oxidation product of DA forms a non-conducting polymer on the electrode surface. In this work several thiols were screened for their ability to prevent this polymerization. It was found that mercaptopropionic acid (MPA) decreased the rate of DA polymerization. MPA, possessing a weak acidic functionality, had the greatest effect on the DA electrochemistry by decreasing electrode passivation, as well as improving reversibility and sensitivity. Modifications of microchip electrodes with MPA did not only improve DA electrochemistry but also significantly increased the storage stability of the transducers. The microchips were ultimately used to detect K+ stimulated quantal release of DA from PC12 cells. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
exocytosis, thiols, microchip, dopamine, PC12 cells, gold
in
Electroanalysis
volume
19
issue
2-3
pages
263 - 271
publisher
John Wiley & Sons
external identifiers
  • wos:000244220700021
  • scopus:34547560083
ISSN
1040-0397
DOI
10.1002/elan.200603720
language
English
LU publication?
yes
id
e77e1b81-70cd-474d-a5eb-05881aa85960 (old id 674672)
date added to LUP
2007-12-19 09:30:32
date last changed
2017-08-27 05:38:24
@article{e77e1b81-70cd-474d-a5eb-05881aa85960,
  abstract     = {Gold and platinum, which often are used for thin film metallization, are not suitable for the measurement of dopamine (DA), since the oxidation product of DA forms a non-conducting polymer on the electrode surface. In this work several thiols were screened for their ability to prevent this polymerization. It was found that mercaptopropionic acid (MPA) decreased the rate of DA polymerization. MPA, possessing a weak acidic functionality, had the greatest effect on the DA electrochemistry by decreasing electrode passivation, as well as improving reversibility and sensitivity. Modifications of microchip electrodes with MPA did not only improve DA electrochemistry but also significantly increased the storage stability of the transducers. The microchips were ultimately used to detect K+ stimulated quantal release of DA from PC12 cells.},
  author       = {Spegel, Christer and Heiskanen, Arto and Acklid, Jenny and Wolff, Anders and Taboryski, Rafael and Emnéus, Jenny and Ruzgas, Tautgirdas},
  issn         = {1040-0397},
  keyword      = {exocytosis,thiols,microchip,dopamine,PC12 cells,gold},
  language     = {eng},
  number       = {2-3},
  pages        = {263--271},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {On-chip determination of dopamine exocytosis using mercaptopropionic acid modified microelectrodes},
  url          = {http://dx.doi.org/10.1002/elan.200603720},
  volume       = {19},
  year         = {2007},
}