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Neurite guidance on protein micropatterns generated by a piezoelectric microdispenser

Gustavsson, Per LU ; Johansson, Fredrik I LU ; Kanje, Martin LU ; Wallman, Lars LU and Eriksson Linsmeier, Cecilia LU (2007) In Biomaterials 28(6). p.1141-1151
Abstract
In this study, we developed a microdispenser technique in order to create protein patterns for guidance of neurites from cultured adult mouse dorsal root ganglia (DRG). The microdispenser is a micromachined silicon device that ejects 100 picolitre droplets and has the ability to position the droplets with a precision of 6-8 mu m. Laminin and bovine serum albumin (BSA) was used to create adhesive and non-adhesive protein lines on polystyrene surfaces (cell culture dishes). Whole-mounted DRGs were then positioned close to the patterns and neurite outgrowth was monitored. The neurites preferred to grow on laminin lines as compared to the unpatterned plastic. When patterns were made from BSA the neurites preferred to grow in between the lines... (More)
In this study, we developed a microdispenser technique in order to create protein patterns for guidance of neurites from cultured adult mouse dorsal root ganglia (DRG). The microdispenser is a micromachined silicon device that ejects 100 picolitre droplets and has the ability to position the droplets with a precision of 6-8 mu m. Laminin and bovine serum albumin (BSA) was used to create adhesive and non-adhesive protein lines on polystyrene surfaces (cell culture dishes). Whole-mounted DRGs were then positioned close to the patterns and neurite outgrowth was monitored. The neurites preferred to grow on laminin lines as compared to the unpatterned plastic. When patterns were made from BSA the neurites preferred to grow in between the lines on the unpatterned plastic surface. We conclude that microdispensing can be used for guidance of sensory neurites. The advantages of microdispensing is that it is fast, flexible, allows deposition of different protein concentrations and enables patterning on delicate surfaces due to its non-contact mode of operation. It is conceivable that microdispensing can be utilized for the creation of protein patterns for guiding neurites to obtain in vitro neural networks, in tissue engineering or rapid screening for guiding proteins. (c) 2006 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
protein, nerve regeneration, micropatterning, nerve tissue engineering, cell adhesion
in
Biomaterials
volume
28
issue
6
pages
1141 - 1151
publisher
Elsevier
external identifiers
  • wos:000243219000025
  • scopus:33751320188
ISSN
1878-5905
DOI
10.1016/j.biomaterials.2006.10.028
language
English
LU publication?
yes
id
e8ec5c1e-2479-4d6b-9198-2e283049be18 (old id 680404)
date added to LUP
2007-12-10 16:07:47
date last changed
2017-01-01 04:42:56
@article{e8ec5c1e-2479-4d6b-9198-2e283049be18,
  abstract     = {In this study, we developed a microdispenser technique in order to create protein patterns for guidance of neurites from cultured adult mouse dorsal root ganglia (DRG). The microdispenser is a micromachined silicon device that ejects 100 picolitre droplets and has the ability to position the droplets with a precision of 6-8 mu m. Laminin and bovine serum albumin (BSA) was used to create adhesive and non-adhesive protein lines on polystyrene surfaces (cell culture dishes). Whole-mounted DRGs were then positioned close to the patterns and neurite outgrowth was monitored. The neurites preferred to grow on laminin lines as compared to the unpatterned plastic. When patterns were made from BSA the neurites preferred to grow in between the lines on the unpatterned plastic surface. We conclude that microdispensing can be used for guidance of sensory neurites. The advantages of microdispensing is that it is fast, flexible, allows deposition of different protein concentrations and enables patterning on delicate surfaces due to its non-contact mode of operation. It is conceivable that microdispensing can be utilized for the creation of protein patterns for guiding neurites to obtain in vitro neural networks, in tissue engineering or rapid screening for guiding proteins. (c) 2006 Elsevier Ltd. All rights reserved.},
  author       = {Gustavsson, Per and Johansson, Fredrik I and Kanje, Martin and Wallman, Lars and Eriksson Linsmeier, Cecilia},
  issn         = {1878-5905},
  keyword      = {protein,nerve regeneration,micropatterning,nerve tissue engineering,cell adhesion},
  language     = {eng},
  number       = {6},
  pages        = {1141--1151},
  publisher    = {Elsevier},
  series       = {Biomaterials},
  title        = {Neurite guidance on protein micropatterns generated by a piezoelectric microdispenser},
  url          = {http://dx.doi.org/10.1016/j.biomaterials.2006.10.028},
  volume       = {28},
  year         = {2007},
}