Neurite guidance on protein micropatterns generated by a piezoelectric microdispenser
(2007) In Biomaterials 28(6). p.1141-1151- Abstract
- In this study, we developed a microdispenser technique in order to create protein patterns for guidance of neurites from cultured adult mouse dorsal root ganglia (DRG). The microdispenser is a micromachined silicon device that ejects 100 picolitre droplets and has the ability to position the droplets with a precision of 6-8 mu m. Laminin and bovine serum albumin (BSA) was used to create adhesive and non-adhesive protein lines on polystyrene surfaces (cell culture dishes). Whole-mounted DRGs were then positioned close to the patterns and neurite outgrowth was monitored. The neurites preferred to grow on laminin lines as compared to the unpatterned plastic. When patterns were made from BSA the neurites preferred to grow in between the lines... (More)
- In this study, we developed a microdispenser technique in order to create protein patterns for guidance of neurites from cultured adult mouse dorsal root ganglia (DRG). The microdispenser is a micromachined silicon device that ejects 100 picolitre droplets and has the ability to position the droplets with a precision of 6-8 mu m. Laminin and bovine serum albumin (BSA) was used to create adhesive and non-adhesive protein lines on polystyrene surfaces (cell culture dishes). Whole-mounted DRGs were then positioned close to the patterns and neurite outgrowth was monitored. The neurites preferred to grow on laminin lines as compared to the unpatterned plastic. When patterns were made from BSA the neurites preferred to grow in between the lines on the unpatterned plastic surface. We conclude that microdispensing can be used for guidance of sensory neurites. The advantages of microdispensing is that it is fast, flexible, allows deposition of different protein concentrations and enables patterning on delicate surfaces due to its non-contact mode of operation. It is conceivable that microdispensing can be utilized for the creation of protein patterns for guiding neurites to obtain in vitro neural networks, in tissue engineering or rapid screening for guiding proteins. (c) 2006 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/680404
- author
- Gustavsson, Per LU ; Johansson, Fredrik I LU ; Kanje, Martin LU ; Wallman, Lars LU and Eriksson Linsmeier, Cecilia LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- protein, nerve regeneration, micropatterning, nerve tissue engineering, cell adhesion
- in
- Biomaterials
- volume
- 28
- issue
- 6
- pages
- 1141 - 1151
- publisher
- Elsevier
- external identifiers
-
- wos:000243219000025
- scopus:33751320188
- ISSN
- 1878-5905
- DOI
- 10.1016/j.biomaterials.2006.10.028
- language
- English
- LU publication?
- yes
- id
- e8ec5c1e-2479-4d6b-9198-2e283049be18 (old id 680404)
- date added to LUP
- 2016-04-01 11:58:18
- date last changed
- 2022-01-26 20:55:06
@article{e8ec5c1e-2479-4d6b-9198-2e283049be18,
abstract = {{In this study, we developed a microdispenser technique in order to create protein patterns for guidance of neurites from cultured adult mouse dorsal root ganglia (DRG). The microdispenser is a micromachined silicon device that ejects 100 picolitre droplets and has the ability to position the droplets with a precision of 6-8 mu m. Laminin and bovine serum albumin (BSA) was used to create adhesive and non-adhesive protein lines on polystyrene surfaces (cell culture dishes). Whole-mounted DRGs were then positioned close to the patterns and neurite outgrowth was monitored. The neurites preferred to grow on laminin lines as compared to the unpatterned plastic. When patterns were made from BSA the neurites preferred to grow in between the lines on the unpatterned plastic surface. We conclude that microdispensing can be used for guidance of sensory neurites. The advantages of microdispensing is that it is fast, flexible, allows deposition of different protein concentrations and enables patterning on delicate surfaces due to its non-contact mode of operation. It is conceivable that microdispensing can be utilized for the creation of protein patterns for guiding neurites to obtain in vitro neural networks, in tissue engineering or rapid screening for guiding proteins. (c) 2006 Elsevier Ltd. All rights reserved.}},
author = {{Gustavsson, Per and Johansson, Fredrik I and Kanje, Martin and Wallman, Lars and Eriksson Linsmeier, Cecilia}},
issn = {{1878-5905}},
keywords = {{protein; nerve regeneration; micropatterning; nerve tissue engineering; cell adhesion}},
language = {{eng}},
number = {{6}},
pages = {{1141--1151}},
publisher = {{Elsevier}},
series = {{Biomaterials}},
title = {{Neurite guidance on protein micropatterns generated by a piezoelectric microdispenser}},
url = {{http://dx.doi.org/10.1016/j.biomaterials.2006.10.028}},
doi = {{10.1016/j.biomaterials.2006.10.028}},
volume = {{28}},
year = {{2007}},
}