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Frequency-dependent diffusion–relaxation distribution MRI : Scan–rescan reproducibility ex vivo and caveats

Or, Pak Shing Kenneth LU ; Yon, Maxime LU ; Narvaez, Omar ; Manninen, Eppu ; Malm, Tarja ; Sierra, Alejandra ; Topgaard, Daniel LU and Benjamini, Dan LU (2026) In NMR in Biomedicine 39(2).
Abstract
Frequency-dependent diffusion–relaxation distribution MRI provides
information beyond the traditional voxel-averaged metric that may better
characterize the microstructural features of biological tissue.
Frequency-dependent multidimensional (ωMD)
MRI reproducibility has been established in clinical settings, but has
yet to be thoroughly evaluated under preclinical conditions, where
superior hardware and modulated gradient waveforms enhance its
performance. In this study, we investigate the reproducibility of ωMD-MRI
using a micro-imaging system to investigate ex vivo mouse brains.
Notably, the estimated signal fractions of intra-voxel spectral
components in the MD-MRI
distribution,... (More)
Frequency-dependent diffusion–relaxation distribution MRI provides
information beyond the traditional voxel-averaged metric that may better
characterize the microstructural features of biological tissue.
Frequency-dependent multidimensional (ωMD)
MRI reproducibility has been established in clinical settings, but has
yet to be thoroughly evaluated under preclinical conditions, where
superior hardware and modulated gradient waveforms enhance its
performance. In this study, we investigate the reproducibility of ωMD-MRI
using a micro-imaging system to investigate ex vivo mouse brains.
Notably, the estimated signal fractions of intra-voxel spectral
components in the MD-MRI
distribution, corresponding to white and gray matter, along with the
frequency-dependent parameters, demonstrated high reproducibility. We
identified bias between scan and rescan in some of the metrics, which we
attribute to the time gap between repeated scans pointing to a
long-time progressive fixation effect. We compare our results with
in vivo results from clinical scanners and show the reproducibility of
diffusion frequency-dependent metrics to benefit from the improved
gradient hardware on our preclinical setup. Our results inform future
micro-imaging ex vivo studies of the reproducibility of ωMD-MRI metrics and their dependence on fixation time. (Less)
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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
diffusion MRI, frequency-dependent diffusion, multidimensional MRI, oscillating gradients (OGSE), reproducibility, tensor valued diffusion encoding
in
NMR in Biomedicine
volume
39
issue
2
article number
e70213
pages
16 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:105025834300
  • pmid:41449779
ISSN
0952-3480
DOI
10.1002/nbm.70213
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2025 The Author(s). NMR in Biomedicine published by John Wiley & Sons Ltd. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
id
682b933d-0c3b-47fc-8c94-06026a540894
date added to LUP
2026-03-05 12:12:43
date last changed
2026-06-12 02:39:03
@article{682b933d-0c3b-47fc-8c94-06026a540894,
  abstract     = {{Frequency-dependent diffusion–relaxation distribution MRI provides <br>
information beyond the traditional voxel-averaged metric that may better<br>
 characterize the microstructural features of biological tissue. <br>
Frequency-dependent multidimensional (ωMD)<br>
 MRI reproducibility has been established in clinical settings, but has <br>
yet to be thoroughly evaluated under preclinical conditions, where <br>
superior hardware and modulated gradient waveforms enhance its <br>
performance. In this study, we investigate the reproducibility of ωMD-MRI<br>
 using a micro-imaging system to investigate ex vivo mouse brains. <br>
Notably, the estimated signal fractions of intra-voxel spectral <br>
components in the MD-MRI<br>
 distribution, corresponding to white and gray matter, along with the <br>
frequency-dependent parameters, demonstrated high reproducibility. We <br>
identified bias between scan and rescan in some of the metrics, which we<br>
 attribute to the time gap between repeated scans pointing to a <br>
long-time progressive fixation effect. We compare our results with <br>
in vivo results from clinical scanners and show the reproducibility of <br>
diffusion frequency-dependent metrics to benefit from the improved <br>
gradient hardware on our preclinical setup. Our results inform future <br>
micro-imaging ex vivo studies of the reproducibility of ωMD-MRI metrics and their dependence on fixation time.}},
  author       = {{Or, Pak Shing Kenneth and Yon, Maxime and Narvaez, Omar and Manninen, Eppu and Malm, Tarja and Sierra, Alejandra and Topgaard, Daniel and Benjamini, Dan}},
  issn         = {{0952-3480}},
  keywords     = {{diffusion MRI; frequency-dependent diffusion; multidimensional MRI; oscillating gradients (OGSE); reproducibility; tensor valued diffusion encoding}},
  language     = {{eng}},
  number       = {{2}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{NMR in Biomedicine}},
  title        = {{Frequency-dependent diffusion–relaxation distribution MRI : Scan–rescan reproducibility ex vivo and caveats}},
  url          = {{http://dx.doi.org/10.1002/nbm.70213}},
  doi          = {{10.1002/nbm.70213}},
  volume       = {{39}},
  year         = {{2026}},
}