Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Transcription factor profiling in individual hematopoietic progenitors by digital RT-PCR

Warren, Luigi ; Bryder, David LU ; Weissman, Irving L. and Quake, Stephen R. (2006) In Proceedings of the National Academy of Sciences 103(47). p.17807-17812
Abstract
We report here a systematic, quantitative population analysis of transcription factor expression within developmental progenitors, made possible by a microfluidic chip-based "digital RT-PCR" assay that can count template molecules in cDNA samples prepared from single cells. In a survey encompassing five classes of early hematopoietic precursor, we found markedly heterogeneous expression of the transcription factor PU.1 in hematopoietic stem cells and divergent patterns of PU.1 expression within flk2(-) and flk2(+) common myeloid progenitors. The survey also revealed significant differences in the level of the housekeeping transcript GAPDH across the surveyed populations, which demonstrates caveats of normalizing expression data to... (More)
We report here a systematic, quantitative population analysis of transcription factor expression within developmental progenitors, made possible by a microfluidic chip-based "digital RT-PCR" assay that can count template molecules in cDNA samples prepared from single cells. In a survey encompassing five classes of early hematopoietic precursor, we found markedly heterogeneous expression of the transcription factor PU.1 in hematopoietic stem cells and divergent patterns of PU.1 expression within flk2(-) and flk2(+) common myeloid progenitors. The survey also revealed significant differences in the level of the housekeeping transcript GAPDH across the surveyed populations, which demonstrates caveats of normalizing expression data to endogenous controls and underscores the need to put gene measurement on an absolute, copy-per-cell basis. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
PU.1, microfluidics, gene profiling, hematopoiesis, stem cells
in
Proceedings of the National Academy of Sciences
volume
103
issue
47
pages
17807 - 17812
publisher
National Acad Sciences
external identifiers
  • wos:000242464900042
  • scopus:33845218548
  • pmid:17098862
ISSN
1091-6490
DOI
10.1073/pnas.0608512103
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematopoietic Stem Cell Laboratory (013022012), Division of Molecular Hematology (DMH) (013017011)
id
06a37421-6cee-421b-b17f-f4366b01270c (old id 683906)
date added to LUP
2016-04-01 12:37:56
date last changed
2020-12-08 03:15:28
@article{06a37421-6cee-421b-b17f-f4366b01270c,
  abstract     = {We report here a systematic, quantitative population analysis of transcription factor expression within developmental progenitors, made possible by a microfluidic chip-based "digital RT-PCR" assay that can count template molecules in cDNA samples prepared from single cells. In a survey encompassing five classes of early hematopoietic precursor, we found markedly heterogeneous expression of the transcription factor PU.1 in hematopoietic stem cells and divergent patterns of PU.1 expression within flk2(-) and flk2(+) common myeloid progenitors. The survey also revealed significant differences in the level of the housekeeping transcript GAPDH across the surveyed populations, which demonstrates caveats of normalizing expression data to endogenous controls and underscores the need to put gene measurement on an absolute, copy-per-cell basis.},
  author       = {Warren, Luigi and Bryder, David and Weissman, Irving L. and Quake, Stephen R.},
  issn         = {1091-6490},
  language     = {eng},
  number       = {47},
  pages        = {17807--17812},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences},
  title        = {Transcription factor profiling in individual hematopoietic progenitors by digital RT-PCR},
  url          = {http://dx.doi.org/10.1073/pnas.0608512103},
  doi          = {10.1073/pnas.0608512103},
  volume       = {103},
  year         = {2006},
}