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CART-peptide immunoreactivity in enteric nerves in patients with Hirschsprung's disease

Gunnarsdottir, Anna LU ; Wierup, Nils LU ; Larsson, Lars Torsten LU ; Kuhar, M. J. and Ekblad, Eva LU (2007) In European Journal of Pediatric Surgery 17(3). p.184-189
Abstract
Aims: Cocaine- and amphetamine-regulated transcript (CART)-peptide is found in the brain and participates in the control of feeding behavior. It is also expressed in the peripheral nervous system and is suggested to have neuromodulatory and/or neurotrophic effects in rat intestine. The aims of this study were to investigate the presence of CART-peptide in the normal ganglionic as well as aganglionic intestine from patients with Hirschsprung's disease and the pepticle's possible coexistence with other neurotransmitters. Methods: Intestinal specimens from nine patients with Hirschsprung's disease were examined using immunohistochemistry. A double immunostaining technique was used in order to elucidate the presence of CART-peptide in NOS and... (More)
Aims: Cocaine- and amphetamine-regulated transcript (CART)-peptide is found in the brain and participates in the control of feeding behavior. It is also expressed in the peripheral nervous system and is suggested to have neuromodulatory and/or neurotrophic effects in rat intestine. The aims of this study were to investigate the presence of CART-peptide in the normal ganglionic as well as aganglionic intestine from patients with Hirschsprung's disease and the pepticle's possible coexistence with other neurotransmitters. Methods: Intestinal specimens from nine patients with Hirschsprung's disease were examined using immunohistochemistry. A double immunostaining technique was used in order to elucidate the presence of CART-peptide in NOS and VIP-containing enteric neurons. Results: in ganglionic intestine, CART-peptide was found in numerous nerve fibers, predominantly within the smooth muscle layers and in myenteric nerve cell bodies. A high degree of co-localization of CART with NOS and VIP was seen. Only very few CART immunoreactive nerve fibers and no nerve cell bodies were found in the aganglionic intestine. Conclusions: This is the first report on the presence of CART-peptide in the human intestine. In the ganglionic intestine CART was detected mainly in myenteric neurons, while only very few CART-IR nerve fibers were found in the aganglionic intestine. This, together with the coexistence of CART with NOS and VIP, indicates an intrinsic origin of the CART-containing neurons and suggests that CART may influence NO and VIP-induced effects. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Hirschsprung's disease, gut motility, intestine, CART, neuropeptide, ENS
in
European Journal of Pediatric Surgery
volume
17
issue
3
pages
184 - 189
publisher
Georg Thieme Verlag
external identifiers
  • wos:000248413800006
  • scopus:34547686536
ISSN
1439-359X
DOI
10.1055/s-2007-965164
language
English
LU publication?
yes
id
e3591811-0735-486e-b68e-c7dd61d6278f (old id 688101)
date added to LUP
2007-12-07 13:47:02
date last changed
2017-01-15 03:29:06
@article{e3591811-0735-486e-b68e-c7dd61d6278f,
  abstract     = {Aims: Cocaine- and amphetamine-regulated transcript (CART)-peptide is found in the brain and participates in the control of feeding behavior. It is also expressed in the peripheral nervous system and is suggested to have neuromodulatory and/or neurotrophic effects in rat intestine. The aims of this study were to investigate the presence of CART-peptide in the normal ganglionic as well as aganglionic intestine from patients with Hirschsprung's disease and the pepticle's possible coexistence with other neurotransmitters. Methods: Intestinal specimens from nine patients with Hirschsprung's disease were examined using immunohistochemistry. A double immunostaining technique was used in order to elucidate the presence of CART-peptide in NOS and VIP-containing enteric neurons. Results: in ganglionic intestine, CART-peptide was found in numerous nerve fibers, predominantly within the smooth muscle layers and in myenteric nerve cell bodies. A high degree of co-localization of CART with NOS and VIP was seen. Only very few CART immunoreactive nerve fibers and no nerve cell bodies were found in the aganglionic intestine. Conclusions: This is the first report on the presence of CART-peptide in the human intestine. In the ganglionic intestine CART was detected mainly in myenteric neurons, while only very few CART-IR nerve fibers were found in the aganglionic intestine. This, together with the coexistence of CART with NOS and VIP, indicates an intrinsic origin of the CART-containing neurons and suggests that CART may influence NO and VIP-induced effects.},
  author       = {Gunnarsdottir, Anna and Wierup, Nils and Larsson, Lars Torsten and Kuhar, M. J. and Ekblad, Eva},
  issn         = {1439-359X},
  keyword      = {Hirschsprung's disease,gut motility,intestine,CART,neuropeptide,ENS},
  language     = {eng},
  number       = {3},
  pages        = {184--189},
  publisher    = {Georg Thieme Verlag},
  series       = {European Journal of Pediatric Surgery},
  title        = {CART-peptide immunoreactivity in enteric nerves in patients with Hirschsprung's disease},
  url          = {http://dx.doi.org/10.1055/s-2007-965164},
  volume       = {17},
  year         = {2007},
}