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The cysteine protease inhibitors cystatins inhibit herpes simplex virus type 1-induced apoptosis and virus yield in HEp-2 cells

Peri, Piritta; Hukkanen, Veijo; Nuutila, Kristiina; Saukko, Pekka; Abrahamson, Magnus LU and Vuorinen, Tytti (2007) In Journal of General Virology 88(8). p.2101-2105
Abstract
The role of cystatins in herpes simplex virus (HSV)-induced apoptosis and viral replication has been studied. Human epithelial (HEp-2) cells infected with wild-type HSV-1 (F), with a deletion virus lacking the anti-apoptotic gene Us3 (R7041) or with a deletion virus lacking the anti-apoptotic genes Us3 and ICP4 (d120) were treated with cystatin A, C or D. Cells and culture media were studied at different time points for replicating HSV-1 and for apoptosis. Cystatins C and D inhibited the yield of replicative HSV-1 significantly in HEp-2 cells. In addition, cystatin D inhibited R7041 and d120 virus-induced apoptosis. Moreover, cystatin A inhibited R7041 induced apoptosis. These inhibitory effects of cystatins on virus replication and... (More)
The role of cystatins in herpes simplex virus (HSV)-induced apoptosis and viral replication has been studied. Human epithelial (HEp-2) cells infected with wild-type HSV-1 (F), with a deletion virus lacking the anti-apoptotic gene Us3 (R7041) or with a deletion virus lacking the anti-apoptotic genes Us3 and ICP4 (d120) were treated with cystatin A, C or D. Cells and culture media were studied at different time points for replicating HSV-1 and for apoptosis. Cystatins C and D inhibited the yield of replicative HSV-1 significantly in HEp-2 cells. In addition, cystatin D inhibited R7041 and d120 virus-induced apoptosis. Moreover, cystatin A inhibited R7041 induced apoptosis. These inhibitory effects of cystatins on virus replication and apoptosis are likely to be separate functions. Cystatin D treatment decreased cellular cathepsin B activity in HSV-1 infection, suggesting that cathepsin B is involved in virus-induced apoptosis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of General Virology
volume
88
issue
8
pages
2101 - 2105
publisher
Society for General Microbiology
external identifiers
  • wos:000248828600002
  • scopus:34547569644
ISSN
1465-2099
DOI
10.1099/vir.0.82990-0
language
English
LU publication?
yes
id
44fd313f-ce56-4e59-a9e8-c0674b0ea327 (old id 688944)
date added to LUP
2007-12-17 10:52:02
date last changed
2017-05-21 04:20:52
@article{44fd313f-ce56-4e59-a9e8-c0674b0ea327,
  abstract     = {The role of cystatins in herpes simplex virus (HSV)-induced apoptosis and viral replication has been studied. Human epithelial (HEp-2) cells infected with wild-type HSV-1 (F), with a deletion virus lacking the anti-apoptotic gene Us3 (R7041) or with a deletion virus lacking the anti-apoptotic genes Us3 and ICP4 (d120) were treated with cystatin A, C or D. Cells and culture media were studied at different time points for replicating HSV-1 and for apoptosis. Cystatins C and D inhibited the yield of replicative HSV-1 significantly in HEp-2 cells. In addition, cystatin D inhibited R7041 and d120 virus-induced apoptosis. Moreover, cystatin A inhibited R7041 induced apoptosis. These inhibitory effects of cystatins on virus replication and apoptosis are likely to be separate functions. Cystatin D treatment decreased cellular cathepsin B activity in HSV-1 infection, suggesting that cathepsin B is involved in virus-induced apoptosis.},
  author       = {Peri, Piritta and Hukkanen, Veijo and Nuutila, Kristiina and Saukko, Pekka and Abrahamson, Magnus and Vuorinen, Tytti},
  issn         = {1465-2099},
  language     = {eng},
  number       = {8},
  pages        = {2101--2105},
  publisher    = {Society for General Microbiology},
  series       = {Journal of General Virology},
  title        = {The cysteine protease inhibitors cystatins inhibit herpes simplex virus type 1-induced apoptosis and virus yield in HEp-2 cells},
  url          = {http://dx.doi.org/10.1099/vir.0.82990-0},
  volume       = {88},
  year         = {2007},
}