Nanoconfinement-enhanced conformational response of single DNA molecules to changes in ionic environment
Reisner, Walter ; Beech, Jason LU ; Larsen, Niels B. ; Flyvbjerg, Henrik ; Kristensen, Anders and Tegenfeldt, Jonas LU
(2007)
In Physical Review Letters
99(5).
- Abstract
- We show that the ionic environment plays a critical role in determining the configurational properties of DNA confined in silica nanochannels. The extension of DNA in the nanochannels increases as the ionic strength is reduced, almost tripling over two decades in ionic strength for channels around 100x100 nm in dimension. Surprisingly, we find that the variation of the persistence length alone with ionic strength is not enough to explain our results. The effect is due mainly to increasing self-avoidance created by the reduced screening of electrostatic interactions at low ionic strength. To quantify the increase in self-avoidance, we introduce a new parameter into the de Gennes theory: an effective DNA width that gives the increase in the... (More)
- We show that the ionic environment plays a critical role in determining the configurational properties of DNA confined in silica nanochannels. The extension of DNA in the nanochannels increases as the ionic strength is reduced, almost tripling over two decades in ionic strength for channels around 100x100 nm in dimension. Surprisingly, we find that the variation of the persistence length alone with ionic strength is not enough to explain our results. The effect is due mainly to increasing self-avoidance created by the reduced screening of electrostatic interactions at low ionic strength. To quantify the increase in self-avoidance, we introduce a new parameter into the de Gennes theory: an effective DNA width that gives the increase in the excluded volume due to electrostatic repulsion. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/690773
- author
- Reisner, Walter
; Beech, Jason
LU
; Larsen, Niels B.
; Flyvbjerg, Henrik
; Kristensen, Anders
and Tegenfeldt, Jonas
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Physical Review Letters
- volume
- 99
- issue
- 5
- article number
- 058302
- publisher
- American Physical Society
- external identifiers
-
- wos:000248597500069
- scopus:34547690725
- pmid:17930801
- ISSN
- 1079-7114
- DOI
- 10.1103/PhysRevLett.99.058302
- language
- English
- LU publication?
- yes
- id
- aebed029-b6c2-4759-a861-64df90d926d0 (old id 690773)
- date added to LUP
- 2016-04-01 12:09:52
- date last changed
- 2022-04-21 03:20:31
@article{aebed029-b6c2-4759-a861-64df90d926d0,
abstract = {{We show that the ionic environment plays a critical role in determining the configurational properties of DNA confined in silica nanochannels. The extension of DNA in the nanochannels increases as the ionic strength is reduced, almost tripling over two decades in ionic strength for channels around 100x100 nm in dimension. Surprisingly, we find that the variation of the persistence length alone with ionic strength is not enough to explain our results. The effect is due mainly to increasing self-avoidance created by the reduced screening of electrostatic interactions at low ionic strength. To quantify the increase in self-avoidance, we introduce a new parameter into the de Gennes theory: an effective DNA width that gives the increase in the excluded volume due to electrostatic repulsion.}},
author = {{Reisner, Walter and Beech, Jason and Larsen, Niels B. and Flyvbjerg, Henrik and Kristensen, Anders and Tegenfeldt, Jonas}},
issn = {{1079-7114}},
language = {{eng}},
number = {{5}},
publisher = {{American Physical Society}},
series = {{Physical Review Letters}},
title = {{Nanoconfinement-enhanced conformational response of single DNA molecules to changes in ionic environment}},
url = {{http://dx.doi.org/10.1103/PhysRevLett.99.058302}},
doi = {{10.1103/PhysRevLett.99.058302}},
volume = {{99}},
year = {{2007}},
}