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In vivo and in vitro intragenomic rearrangement of TT viruses

Leppik, Ludmila; Gunst, Karin; Lehtinen, Matti; Dillner, Joakim LU ; Streker, Karin and de Villiers, Ethel-Michele (2007) In Journal of Virology 81(17). p.9346-9356
Abstract
The in vitro replication of the Torque teno virus (IT virus) tth8 full-length genome and particle formation in a Hodgkin's lymphoma-derived cell line after transfection with cloned viral DNA were demonstrated. Analyses of the transcription patterns of tth8 and tth7 TT virus isolates in a number of lymphoma and T-cell leukemia cell lines indicated differential additional splicing events and intragenomic rearrangement generating open reading frames which could not be deducted from the genomic sequence. We also demonstrated the presence of rearranged TT virus genomes in vivo in sera taken from pregnant mothers whose children later developed childhood leukemia, as well as sera from control mothers. Control experiments using religated cloned... (More)
The in vitro replication of the Torque teno virus (IT virus) tth8 full-length genome and particle formation in a Hodgkin's lymphoma-derived cell line after transfection with cloned viral DNA were demonstrated. Analyses of the transcription patterns of tth8 and tth7 TT virus isolates in a number of lymphoma and T-cell leukemia cell lines indicated differential additional splicing events and intragenomic rearrangement generating open reading frames which could not be deducted from the genomic sequence. We also demonstrated the presence of rearranged TT virus genomes in vivo in sera taken from pregnant mothers whose children later developed childhood leukemia, as well as sera from control mothers. Control experiments using religated cloned genomic tth8 DNA mixed with cellular DNA did not result in such subviral molecules. These subviral isolates ranged from 172 bp to full-length TT virus genomes. Possible in vivo selection for specific rearranged molecules was indicated by the presence of one isolate (561 bp) in 11 serum samples. It remains to be clarified whether selected rearranged subviral components resulting from specific Tr virus types may contribute to the initiation of disease. These data demonstrate new features of Tr viruses suggesting possible similarities to plant viruses of the family Geminiviridae, as well as raise questions about the documented plurality and diversity of anelloviruses. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Virology
volume
81
issue
17
pages
9346 - 9356
publisher
American Society for Microbiology
external identifiers
  • wos:000248923700047
  • scopus:34548146497
ISSN
1098-5514
DOI
10.1128/JVI.00781-07
language
English
LU publication?
yes
id
dfc84f96-47d7-43d4-b225-cd9154eecd33 (old id 691572)
date added to LUP
2007-12-14 14:11:06
date last changed
2017-10-22 04:30:59
@article{dfc84f96-47d7-43d4-b225-cd9154eecd33,
  abstract     = {The in vitro replication of the Torque teno virus (IT virus) tth8 full-length genome and particle formation in a Hodgkin's lymphoma-derived cell line after transfection with cloned viral DNA were demonstrated. Analyses of the transcription patterns of tth8 and tth7 TT virus isolates in a number of lymphoma and T-cell leukemia cell lines indicated differential additional splicing events and intragenomic rearrangement generating open reading frames which could not be deducted from the genomic sequence. We also demonstrated the presence of rearranged TT virus genomes in vivo in sera taken from pregnant mothers whose children later developed childhood leukemia, as well as sera from control mothers. Control experiments using religated cloned genomic tth8 DNA mixed with cellular DNA did not result in such subviral molecules. These subviral isolates ranged from 172 bp to full-length TT virus genomes. Possible in vivo selection for specific rearranged molecules was indicated by the presence of one isolate (561 bp) in 11 serum samples. It remains to be clarified whether selected rearranged subviral components resulting from specific Tr virus types may contribute to the initiation of disease. These data demonstrate new features of Tr viruses suggesting possible similarities to plant viruses of the family Geminiviridae, as well as raise questions about the documented plurality and diversity of anelloviruses.},
  author       = {Leppik, Ludmila and Gunst, Karin and Lehtinen, Matti and Dillner, Joakim and Streker, Karin and de Villiers, Ethel-Michele},
  issn         = {1098-5514},
  language     = {eng},
  number       = {17},
  pages        = {9346--9356},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Virology},
  title        = {In vivo and in vitro intragenomic rearrangement of TT viruses},
  url          = {http://dx.doi.org/10.1128/JVI.00781-07},
  volume       = {81},
  year         = {2007},
}