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Differential expression of trypsinogen and tumor-associated trypsin inhibitor (TATI) in bladder cancer

Hotakainen, K ; Bjartell, Anders LU ; Sankila, A ; Jarvinen, R ; Paju, A ; Rintala, E ; Haglund, C and Stenman, UH (2006) In International Journal of Oncology 28(1). p.95-101
Abstract
Tumor-associated trypsin inhibitor (TATI) is a marker of mucinous ovarian carcinoma, but it is also widely expressed in other malignant tumors and normal human tissues. Elevated serum concentrations of TATI are of prognostic value in ovarian, kidney, and bladder cancer. Tumor-associated trypsin is co-expressed with TATI in many malignancies and is thought to be involved in tumor invasion. TATI mRNA has been shown to be overexpressed in bladder cancer. We therefore studied whether trypsinogen expression also can be detected in bladder cancer and how this and TATI expression are associated with the clinicopathological characteristics of the tumors. We used RT-PCR, in situ hybridization and immunohistochemistry to detect trypsinogen-and TATI... (More)
Tumor-associated trypsin inhibitor (TATI) is a marker of mucinous ovarian carcinoma, but it is also widely expressed in other malignant tumors and normal human tissues. Elevated serum concentrations of TATI are of prognostic value in ovarian, kidney, and bladder cancer. Tumor-associated trypsin is co-expressed with TATI in many malignancies and is thought to be involved in tumor invasion. TATI mRNA has been shown to be overexpressed in bladder cancer. We therefore studied whether trypsinogen expression also can be detected in bladder cancer and how this and TATI expression are associated with the clinicopathological characteristics of the tumors. We used RT-PCR, in situ hybridization and immunohistochemistry to detect trypsinogen-and TATI mRNA and protein in tissue samples from 28 bladder cancer patients and ten benign urothelia. TATI expression was detected in all benign tissues and non-invasive tumors. However, the expression was lower in the muscle-invasive tumors (pT2; n=5), whereas trypsinogen expression was seen in all but one non-invasive tumor. We conclude that trypsinogen is expressed in both malignant and benign bladder epithelium, whereas TATI expression decreases with increasing stage and grade of the tumor. This may suggest that a balanced expression of TATI and trypsinogen is required in normal tissue and that this balance is disrupted during tumor progression. (Less)
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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bladder cancer, TATI, trypsinogen, RT-PCR
in
International Journal of Oncology
volume
28
issue
1
pages
95 - 101
publisher
D.A. Spandidos
external identifiers
  • pmid:16327984
  • wos:000234201400010
  • scopus:33644804552
ISSN
1019-6439
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Urology (013243400), Division of Urological Cancers (013243420)
id
71f6baca-4473-4d55-8b12-b79b1a476475 (old id 693751)
date added to LUP
2016-04-01 16:39:27
date last changed
2020-07-22 02:13:19
@article{71f6baca-4473-4d55-8b12-b79b1a476475,
  abstract     = {Tumor-associated trypsin inhibitor (TATI) is a marker of mucinous ovarian carcinoma, but it is also widely expressed in other malignant tumors and normal human tissues. Elevated serum concentrations of TATI are of prognostic value in ovarian, kidney, and bladder cancer. Tumor-associated trypsin is co-expressed with TATI in many malignancies and is thought to be involved in tumor invasion. TATI mRNA has been shown to be overexpressed in bladder cancer. We therefore studied whether trypsinogen expression also can be detected in bladder cancer and how this and TATI expression are associated with the clinicopathological characteristics of the tumors. We used RT-PCR, in situ hybridization and immunohistochemistry to detect trypsinogen-and TATI mRNA and protein in tissue samples from 28 bladder cancer patients and ten benign urothelia. TATI expression was detected in all benign tissues and non-invasive tumors. However, the expression was lower in the muscle-invasive tumors (pT2; n=5), whereas trypsinogen expression was seen in all but one non-invasive tumor. We conclude that trypsinogen is expressed in both malignant and benign bladder epithelium, whereas TATI expression decreases with increasing stage and grade of the tumor. This may suggest that a balanced expression of TATI and trypsinogen is required in normal tissue and that this balance is disrupted during tumor progression.},
  author       = {Hotakainen, K and Bjartell, Anders and Sankila, A and Jarvinen, R and Paju, A and Rintala, E and Haglund, C and Stenman, UH},
  issn         = {1019-6439},
  language     = {eng},
  number       = {1},
  pages        = {95--101},
  publisher    = {D.A. Spandidos},
  series       = {International Journal of Oncology},
  title        = {Differential expression of trypsinogen and tumor-associated trypsin inhibitor (TATI) in bladder cancer},
  volume       = {28},
  year         = {2006},
}