Two-photon excitation in fluorescence polarization receptor-ligand binding assay
(2005) In Journal of Biomolecular Screening 10(4). p.314-319- Abstract
- Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1133685
- author
- Tirri, M E ; Huttunen, R J ; Toivonen, J ; Härkönen, Pirkko LU ; Soini, J T and Hanninen, P E
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- two-photon excitation, fluorescence polarization, high-throughput screening, estrogen receptor, drug discovery
- in
- Journal of Biomolecular Screening
- volume
- 10
- issue
- 4
- pages
- 314 - 319
- publisher
- SAGE Publications
- external identifiers
-
- scopus:20444425639
- ISSN
- 1087-0571
- DOI
- 10.1177/1087057104273334
- language
- English
- LU publication?
- yes
- additional info
- Department affilation moved from v1000588 (Tumour Biology, Malmö) to v1000562 (Department of Translational Medicine) on 2016-01-18 14:39:32.
- id
- 696fc8f7-d1a6-44a3-86c6-9d05b967b731 (old id 1133685)
- date added to LUP
- 2016-04-01 12:23:34
- date last changed
- 2022-04-21 06:48:48
@article{696fc8f7-d1a6-44a3-86c6-9d05b967b731, abstract = {{Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay.}}, author = {{Tirri, M E and Huttunen, R J and Toivonen, J and Härkönen, Pirkko and Soini, J T and Hanninen, P E}}, issn = {{1087-0571}}, keywords = {{two-photon excitation; fluorescence polarization; high-throughput screening; estrogen receptor; drug discovery}}, language = {{eng}}, number = {{4}}, pages = {{314--319}}, publisher = {{SAGE Publications}}, series = {{Journal of Biomolecular Screening}}, title = {{Two-photon excitation in fluorescence polarization receptor-ligand binding assay}}, url = {{http://dx.doi.org/10.1177/1087057104273334}}, doi = {{10.1177/1087057104273334}}, volume = {{10}}, year = {{2005}}, }