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Peptide and Gd Complexes Containing Colloidal Assemblies as Tumor-Specific Contrast Agents in MRI: Physicochemical Characterization

Vaccaro, Mauro LU ; Accardo, Antonella; D'Errico, Gerardino; Schillén, Karin LU ; Radulescu, Aurel; Tesauro, Diego; Morelli, Giancarlo and Paduano, Luigi (2007) In Biophysical Journal 93(5). p.1736-1746
Abstract
The aggregation behavior of an amphiphilic supramolecular system, with potential application as a tumor-specific magnetic resonance imaging contrast agent, has been studied in detail by dynamic light scattering, small-angle neutron scattering and cryotransmission electron microscopy. The system was constituted of mixed aggregates formed by an anionic unimer containing the DTPAGlu, a chelating agent for the paramagnetic Gd3+ ion, and an uncharged unimer containing the bioactive peptide CCK8, capable of directing the assembly toward tumor cells. Mixed aggregates formed by both unimers, and in the case of the DTPAGlu unimer with the chelating agent as free base or as Gd3+ complex, have been investigated. A number of interesting features of... (More)
The aggregation behavior of an amphiphilic supramolecular system, with potential application as a tumor-specific magnetic resonance imaging contrast agent, has been studied in detail by dynamic light scattering, small-angle neutron scattering and cryotransmission electron microscopy. The system was constituted of mixed aggregates formed by an anionic unimer containing the DTPAGlu, a chelating agent for the paramagnetic Gd3+ ion, and an uncharged unimer containing the bioactive peptide CCK8, capable of directing the assembly toward tumor cells. Mixed aggregates formed by both unimers, and in the case of the DTPAGlu unimer with the chelating agent as free base or as Gd3+ complex, have been investigated. A number of interesting features of the aggregation behavior were revealed: at physiological pH, micelles and bilayer structures were present, whereas upon decreasing solution pH or increasing ionic strength, the formation of bilayer structures was favored. On the basis of the above observations, the aggregating mechanism has been elucidated by considering the screening effect on intra- and interaggregate electrostatic repulsions. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
SYSTEM, RECEPTORS, DIFFUSION, HIGH-RELAXIVITY, AQUEOUS-SOLUTION, LIGHT-SCATTERING, ANGLE NEUTRON-SCATTERING
in
Biophysical Journal
volume
93
issue
5
pages
1736 - 1746
publisher
Cell Press
external identifiers
  • wos:000248722200029
  • scopus:34548603625
ISSN
1542-0086
DOI
10.1529/biophysj.107.107417
language
English
LU publication?
yes
id
f979f958-28db-409b-aa2c-d9be7fd9adde (old id 697175)
alternative location
http://proquest.umi.com/pqdlink?did=1324488261&sid=4&Fmt=4&clientId=53681&RQT=309&VName=PQD
date added to LUP
2008-01-09 11:02:43
date last changed
2017-01-01 05:07:26
@article{f979f958-28db-409b-aa2c-d9be7fd9adde,
  abstract     = {The aggregation behavior of an amphiphilic supramolecular system, with potential application as a tumor-specific magnetic resonance imaging contrast agent, has been studied in detail by dynamic light scattering, small-angle neutron scattering and cryotransmission electron microscopy. The system was constituted of mixed aggregates formed by an anionic unimer containing the DTPAGlu, a chelating agent for the paramagnetic Gd3+ ion, and an uncharged unimer containing the bioactive peptide CCK8, capable of directing the assembly toward tumor cells. Mixed aggregates formed by both unimers, and in the case of the DTPAGlu unimer with the chelating agent as free base or as Gd3+ complex, have been investigated. A number of interesting features of the aggregation behavior were revealed: at physiological pH, micelles and bilayer structures were present, whereas upon decreasing solution pH or increasing ionic strength, the formation of bilayer structures was favored. On the basis of the above observations, the aggregating mechanism has been elucidated by considering the screening effect on intra- and interaggregate electrostatic repulsions.},
  author       = {Vaccaro, Mauro and Accardo, Antonella and D'Errico, Gerardino and Schillén, Karin and Radulescu, Aurel and Tesauro, Diego and Morelli, Giancarlo and Paduano, Luigi},
  issn         = {1542-0086},
  keyword      = {SYSTEM,RECEPTORS,DIFFUSION,HIGH-RELAXIVITY,AQUEOUS-SOLUTION,LIGHT-SCATTERING,ANGLE NEUTRON-SCATTERING},
  language     = {eng},
  number       = {5},
  pages        = {1736--1746},
  publisher    = {Cell Press},
  series       = {Biophysical Journal},
  title        = {Peptide and Gd Complexes Containing Colloidal Assemblies as Tumor-Specific Contrast Agents in MRI: Physicochemical Characterization},
  url          = {http://dx.doi.org/10.1529/biophysj.107.107417},
  volume       = {93},
  year         = {2007},
}