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Label-free detection of polystyrene nanoparticles in Daphnia magna using Raman confocal mapping

Kaur, Jasreen ; Kelpsiene, Egle LU ; Gupta, Govind ; Dobryden, Illia ; Cedervall, Tommy LU and Fadeel, Bengt (2023) In Nanoscale Advances 5(13). p.3453-3462
Abstract

Micro- and nanoplastic pollution has emerged as a global environmental problem. Moreover, plastic particles are of increasing concern for human health. However, the detection of so-called nanoplastics in relevant biological compartments remains a challenge. Here we show that Raman confocal spectroscopy-microscopy can be deployed for the non-invasive detection of amine-functionalized and carboxy-functionalized polystyrene (PS) nanoparticles (NPs) in Daphnia magna. The presence of PS NPs in the gastrointestinal (GI) tract of D. magna was confirmed by using transmission electron microscopy. Furthermore, we investigated the ability of NH2-PS NPs and COOH-PS NPs to disrupt the epithelial barrier of the GI tract using the human... (More)

Micro- and nanoplastic pollution has emerged as a global environmental problem. Moreover, plastic particles are of increasing concern for human health. However, the detection of so-called nanoplastics in relevant biological compartments remains a challenge. Here we show that Raman confocal spectroscopy-microscopy can be deployed for the non-invasive detection of amine-functionalized and carboxy-functionalized polystyrene (PS) nanoparticles (NPs) in Daphnia magna. The presence of PS NPs in the gastrointestinal (GI) tract of D. magna was confirmed by using transmission electron microscopy. Furthermore, we investigated the ability of NH2-PS NPs and COOH-PS NPs to disrupt the epithelial barrier of the GI tract using the human colon adenocarcinoma cell line HT-29. To this end, the cells were differentiated for 21 days and then exposed to PS NPs followed by cytotoxicity assessment and transepithelial electrical resistance measurements. A minor disruption of barrier integrity was noted for COOH-PS NPs, but not for the NH2-PS NPs, while no overt cytotoxicity was observed for both NPs. This study provides evidence of the feasibility of applying label-free approaches, i.e., confocal Raman mapping, to study PS NPs in a biological system.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nanoscale Advances
volume
5
issue
13
pages
10 pages
publisher
Royal Society of Chemistry
external identifiers
  • pmid:37383076
  • scopus:85161612138
ISSN
2516-0230
DOI
10.1039/d3na00323j
language
English
LU publication?
yes
id
69c87acb-4935-4dcb-8185-6ef59f922914
date added to LUP
2023-08-04 09:54:51
date last changed
2024-04-20 00:07:42
@article{69c87acb-4935-4dcb-8185-6ef59f922914,
  abstract     = {{<p>Micro- and nanoplastic pollution has emerged as a global environmental problem. Moreover, plastic particles are of increasing concern for human health. However, the detection of so-called nanoplastics in relevant biological compartments remains a challenge. Here we show that Raman confocal spectroscopy-microscopy can be deployed for the non-invasive detection of amine-functionalized and carboxy-functionalized polystyrene (PS) nanoparticles (NPs) in Daphnia magna. The presence of PS NPs in the gastrointestinal (GI) tract of D. magna was confirmed by using transmission electron microscopy. Furthermore, we investigated the ability of NH<sub>2</sub>-PS NPs and COOH-PS NPs to disrupt the epithelial barrier of the GI tract using the human colon adenocarcinoma cell line HT-29. To this end, the cells were differentiated for 21 days and then exposed to PS NPs followed by cytotoxicity assessment and transepithelial electrical resistance measurements. A minor disruption of barrier integrity was noted for COOH-PS NPs, but not for the NH<sub>2</sub>-PS NPs, while no overt cytotoxicity was observed for both NPs. This study provides evidence of the feasibility of applying label-free approaches, i.e., confocal Raman mapping, to study PS NPs in a biological system.</p>}},
  author       = {{Kaur, Jasreen and Kelpsiene, Egle and Gupta, Govind and Dobryden, Illia and Cedervall, Tommy and Fadeel, Bengt}},
  issn         = {{2516-0230}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{13}},
  pages        = {{3453--3462}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Nanoscale Advances}},
  title        = {{Label-free detection of polystyrene nanoparticles in Daphnia magna using Raman confocal mapping}},
  url          = {{http://dx.doi.org/10.1039/d3na00323j}},
  doi          = {{10.1039/d3na00323j}},
  volume       = {{5}},
  year         = {{2023}},
}