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Seroepidemiology of the human polyomaviruses.

Stolt, Annika; Sasnauskas, Kestutis; Koskela, Pentti; Lehtinen, Matti and Dillner, Joakim LU (2003) In Journal of General Virology 84(Pt 6). p.1499-1504
Abstract
To assess the stability of polyomavirus antibodies in serial samples over time and the incidence and age-specific prevalence of polyomavirus infections, we established enzyme immunoassays (EIAs) using purified yeast-expressed virus-like particles (VLPs) containing the VP1 major capsid proteins of JC virus (JCV) and the AS and SB strains of BK virus (BKV). A random subsample of 150 Finnish women who had serum samples taken during the first trimester of pregnancy and had a second pregnancy during a 5 year follow-up period was selected, grouped by age of first pregnancy. The polyomavirus antibody levels were similar in samples taken during the first and second pregnancies (correlation coefficient 0·93 for BKV SB and 0·94 for JCV). Analysis of... (More)
To assess the stability of polyomavirus antibodies in serial samples over time and the incidence and age-specific prevalence of polyomavirus infections, we established enzyme immunoassays (EIAs) using purified yeast-expressed virus-like particles (VLPs) containing the VP1 major capsid proteins of JC virus (JCV) and the AS and SB strains of BK virus (BKV). A random subsample of 150 Finnish women who had serum samples taken during the first trimester of pregnancy and had a second pregnancy during a 5 year follow-up period was selected, grouped by age of first pregnancy. The polyomavirus antibody levels were similar in samples taken during the first and second pregnancies (correlation coefficient 0·93 for BKV SB and 0·94 for JCV). Analysis of serum samples from 290 Swedish children aged 1–13 years, grouped by age in 2 year intervals, demonstrated that BKV seropositivity increased rapidly with increasing age of the children, reaching 98 % seroprevalence at 7–9 years of age, followed by a minor decrease. JCV seroprevalence increased only slowly with increasing age and reaching 72 % positivity among mothers >25 years of age. The age-specific seroprevalence of the human polyomaviruses measured using this VLP-based EIA was similar to previous serosurveys by other methods. The stability of the antibodies over time indicates that polyomavirus seropositivity is a valid marker of cumulative virus exposure, and polyoma VLP-based EIAs may therefore be useful for epidemiological studies of these viruses. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of General Virology
volume
84
issue
Pt 6
pages
1499 - 1504
publisher
Society for General Microbiology
external identifiers
  • wos:000183372300018
  • pmid:12771419
  • scopus:0038654083
ISSN
1465-2099
DOI
10.1099/vir.0.18842-0
language
English
LU publication?
yes
id
6a18e12e-3e20-437b-9f63-37c8f8d085b5 (old id 116277)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12771419&dopt=Abstract
date added to LUP
2007-07-27 10:43:53
date last changed
2018-05-06 03:58:36
@article{6a18e12e-3e20-437b-9f63-37c8f8d085b5,
  abstract     = {To assess the stability of polyomavirus antibodies in serial samples over time and the incidence and age-specific prevalence of polyomavirus infections, we established enzyme immunoassays (EIAs) using purified yeast-expressed virus-like particles (VLPs) containing the VP1 major capsid proteins of JC virus (JCV) and the AS and SB strains of BK virus (BKV). A random subsample of 150 Finnish women who had serum samples taken during the first trimester of pregnancy and had a second pregnancy during a 5 year follow-up period was selected, grouped by age of first pregnancy. The polyomavirus antibody levels were similar in samples taken during the first and second pregnancies (correlation coefficient 0·93 for BKV SB and 0·94 for JCV). Analysis of serum samples from 290 Swedish children aged 1–13 years, grouped by age in 2 year intervals, demonstrated that BKV seropositivity increased rapidly with increasing age of the children, reaching 98 % seroprevalence at 7–9 years of age, followed by a minor decrease. JCV seroprevalence increased only slowly with increasing age and reaching 72 % positivity among mothers >25 years of age. The age-specific seroprevalence of the human polyomaviruses measured using this VLP-based EIA was similar to previous serosurveys by other methods. The stability of the antibodies over time indicates that polyomavirus seropositivity is a valid marker of cumulative virus exposure, and polyoma VLP-based EIAs may therefore be useful for epidemiological studies of these viruses.},
  author       = {Stolt, Annika and Sasnauskas, Kestutis and Koskela, Pentti and Lehtinen, Matti and Dillner, Joakim},
  issn         = {1465-2099},
  language     = {eng},
  number       = {Pt 6},
  pages        = {1499--1504},
  publisher    = {Society for General Microbiology},
  series       = {Journal of General Virology},
  title        = {Seroepidemiology of the human polyomaviruses.},
  url          = {http://dx.doi.org/10.1099/vir.0.18842-0},
  volume       = {84},
  year         = {2003},
}