Establishment and characterization of a human papillomavirus type 16-positive tonsillar carcinoma xenograft in BALB/c nude mice
Letsolo, Boitelo T LU ; Faust, Helena LU ; Ekblad, Lars LU
; Wennerberg, Johan
LU
and Forslund, Ola
LU
(2016)
In Head and Neck
38(3).
p.25-417
- Abstract
BACKGROUND: Among head and neck cancers, human papillomavirus type 16 (HPV16) is associated with tonsillar carcinomas. Despite this, no HPV16-positive tonsillar cancer cell line has been established in nude mice.
METHODS: Fresh tonsillar carcinoma biopsies were obtained from 23 patients and implanted subcutaneously into nude mice (BALB/c, nu/nu).
RESULTS: After 7 months, one xenograft was established. The primary tumor harbored 2.7 copies (95% confidence interval = 2.4-2.9) of HPV16/cell and displayed 99.9% (7904/7906) nucleotide identity to HPV16 (EU118173.1). The xenograft showed increased methylation in two E2-binding sites of the HPV16 genome. Both episomal and integrated HPV16 were detected in the original tumor and in... (More)
BACKGROUND: Among head and neck cancers, human papillomavirus type 16 (HPV16) is associated with tonsillar carcinomas. Despite this, no HPV16-positive tonsillar cancer cell line has been established in nude mice.
METHODS: Fresh tonsillar carcinoma biopsies were obtained from 23 patients and implanted subcutaneously into nude mice (BALB/c, nu/nu).
RESULTS: After 7 months, one xenograft was established. The primary tumor harbored 2.7 copies (95% confidence interval = 2.4-2.9) of HPV16/cell and displayed 99.9% (7904/7906) nucleotide identity to HPV16 (EU118173.1). The xenograft showed increased methylation in two E2-binding sites of the HPV16 genome. Both episomal and integrated HPV16 were detected in the original tumor and in 14 xenografts from the second passage. From this passage, a viral load of 6.4 copies/cell (range = 4.6-9.6) and 3.7 (range = 1.0-5.5) E7-mRNA transcripts/HPV16-genome were detected.
CONCLUSION: This xenograft represents the first established HPV16-positive tonsillar tumor in nude mice and could provide an experimental system of HPV16-positive tonsillar cancers. © 2015 Wiley Periodicals, Inc. Head Neck 38: 417-425, 2016.
(Less)
- author
- Letsolo, Boitelo T
LU
; Faust, Helena
LU
; Ekblad, Lars
LU
; Wennerberg, Johan
LU
and Forslund, Ola
LU
- organization
- publishing date
- 2016-03
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Head and Neck
- volume
- 38
- issue
- 3
- pages
- 9 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:25352201
- scopus:84958212706
- pmid:25352201
- wos:000370187400017
- ISSN
- 1043-3074
- DOI
- 10.1002/hed.23918
- language
- English
- LU publication?
- yes
- id
- 6a8a4d3b-5a81-4d10-80d5-3595d8fee24c (old id 4732991)
- date added to LUP
- 2016-04-04 07:59:53
- date last changed
- 2022-04-07 23:24:03
@article{6a8a4d3b-5a81-4d10-80d5-3595d8fee24c,
abstract = {{<p>BACKGROUND: Among head and neck cancers, human papillomavirus type 16 (HPV16) is associated with tonsillar carcinomas. Despite this, no HPV16-positive tonsillar cancer cell line has been established in nude mice.</p><p>METHODS: Fresh tonsillar carcinoma biopsies were obtained from 23 patients and implanted subcutaneously into nude mice (BALB/c, nu/nu).</p><p>RESULTS: After 7 months, one xenograft was established. The primary tumor harbored 2.7 copies (95% confidence interval = 2.4-2.9) of HPV16/cell and displayed 99.9% (7904/7906) nucleotide identity to HPV16 (EU118173.1). The xenograft showed increased methylation in two E2-binding sites of the HPV16 genome. Both episomal and integrated HPV16 were detected in the original tumor and in 14 xenografts from the second passage. From this passage, a viral load of 6.4 copies/cell (range = 4.6-9.6) and 3.7 (range = 1.0-5.5) E7-mRNA transcripts/HPV16-genome were detected.</p><p>CONCLUSION: This xenograft represents the first established HPV16-positive tonsillar tumor in nude mice and could provide an experimental system of HPV16-positive tonsillar cancers. © 2015 Wiley Periodicals, Inc. Head Neck 38: 417-425, 2016.</p>}},
author = {{Letsolo, Boitelo T and Faust, Helena and Ekblad, Lars and Wennerberg, Johan and Forslund, Ola}},
issn = {{1043-3074}},
language = {{eng}},
number = {{3}},
pages = {{25--417}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Head and Neck}},
title = {{Establishment and characterization of a human papillomavirus type 16-positive tonsillar carcinoma xenograft in BALB/c nude mice}},
url = {{http://dx.doi.org/10.1002/hed.23918}},
doi = {{10.1002/hed.23918}},
volume = {{38}},
year = {{2016}},
}