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Lgr6 is a stem cell marker in mouse skin squamous cell carcinoma

Huang, Phillips Y; Kandyba, Eve; Jabouille, Arnaud; Sjölund, Jonas LU ; Kumar, Atul; Halliwill, Kyle D; McCreery, Melissa; DelRosario, Reyno; Kang, Hio Chung and Wong, Christine E, et al. (2017) In Nature Genetics 49(11). p.1624-1632
Abstract

The G-protein-coupled receptors LGR4, LGR5 and LGR6 are Wnt signaling mediators, but their functions in squamous cell carcinoma (SCC) are unclear. Using lineage tracing in Lgr5-EGFP-CreERT2/Rosa26-Tomato and Lgr6-EGFP-CreERT2/Rosa26-Tomato reporter mice, we demonstrate that Lgr6, but not Lgr5, acts as an epithelial stem cell marker in SCCs in vivo. We identify, by single-molecule in situ hybridization and cell sorting, rare cells positive for Lgr6 expression in immortalized keratinocytes and show that their frequency increases in advanced SCCs. Lgr6 expression is enriched in cells with stem cell characteristics, and Lgr6 downregulation in vivo causes increased epidermal proliferation with expanded lineage tracing from epidermal stem... (More)

The G-protein-coupled receptors LGR4, LGR5 and LGR6 are Wnt signaling mediators, but their functions in squamous cell carcinoma (SCC) are unclear. Using lineage tracing in Lgr5-EGFP-CreERT2/Rosa26-Tomato and Lgr6-EGFP-CreERT2/Rosa26-Tomato reporter mice, we demonstrate that Lgr6, but not Lgr5, acts as an epithelial stem cell marker in SCCs in vivo. We identify, by single-molecule in situ hybridization and cell sorting, rare cells positive for Lgr6 expression in immortalized keratinocytes and show that their frequency increases in advanced SCCs. Lgr6 expression is enriched in cells with stem cell characteristics, and Lgr6 downregulation in vivo causes increased epidermal proliferation with expanded lineage tracing from epidermal stem cells positive for Lgr6 expression. Surprisingly, mice with germline knockout of Lgr6 are predisposed to SCC development, through a mechanism that includes compensatory upregulation of Lgr5. These data provide a model for human patients with germline loss-of-function mutations in Wnt pathway genes, including RSPO1 or LGR4, who show increased susceptibility to squamous tumor development.

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Nature Genetics
volume
49
issue
11
pages
1624 - 1632
publisher
Nature Publishing Group
external identifiers
  • scopus:85032455116
  • wos:000413909800011
ISSN
1546-1718
DOI
10.1038/ng.3957
language
English
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yes
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6aacacbb-62e9-487c-bc57-b5a72d336090
date added to LUP
2017-09-27 10:07:15
date last changed
2018-10-03 10:28:08
@article{6aacacbb-62e9-487c-bc57-b5a72d336090,
  abstract     = {<p>The G-protein-coupled receptors LGR4, LGR5 and LGR6 are Wnt signaling mediators, but their functions in squamous cell carcinoma (SCC) are unclear. Using lineage tracing in Lgr5-EGFP-CreERT2/Rosa26-Tomato and Lgr6-EGFP-CreERT2/Rosa26-Tomato reporter mice, we demonstrate that Lgr6, but not Lgr5, acts as an epithelial stem cell marker in SCCs in vivo. We identify, by single-molecule in situ hybridization and cell sorting, rare cells positive for Lgr6 expression in immortalized keratinocytes and show that their frequency increases in advanced SCCs. Lgr6 expression is enriched in cells with stem cell characteristics, and Lgr6 downregulation in vivo causes increased epidermal proliferation with expanded lineage tracing from epidermal stem cells positive for Lgr6 expression. Surprisingly, mice with germline knockout of Lgr6 are predisposed to SCC development, through a mechanism that includes compensatory upregulation of Lgr5. These data provide a model for human patients with germline loss-of-function mutations in Wnt pathway genes, including RSPO1 or LGR4, who show increased susceptibility to squamous tumor development.</p>},
  author       = {Huang, Phillips Y and Kandyba, Eve and Jabouille, Arnaud and Sjölund, Jonas and Kumar, Atul and Halliwill, Kyle D and McCreery, Melissa and DelRosario, Reyno and Kang, Hio Chung and Wong, Christine E and Seibler, Jost and Beuger, Vincent and Pellegrino, Maurizio and Sciambi, Adam and Eastburn, Dennis J and Balmain, Allan},
  issn         = {1546-1718},
  language     = {eng},
  month        = {09},
  number       = {11},
  pages        = {1624--1632},
  publisher    = {Nature Publishing Group},
  series       = {Nature Genetics},
  title        = {Lgr6 is a stem cell marker in mouse skin squamous cell carcinoma},
  url          = {http://dx.doi.org/10.1038/ng.3957},
  volume       = {49},
  year         = {2017},
}