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Pressurized fluid extraction of bioactive compounds from Phormidium species

Rodríguez-Meizoso, I. LU ; Jaime, Laura; Santoyo, Susana; Cifuentes, Alejandro; García-Blairsy Reina, G.; Señoráns, F. J. and Ibáñez, Elena (2008) In Journal of Agricultural and Food Chemistry 56(10). p.3517-3523
Abstract

In the search for new functional ingredients with potential use in the food industry, extracts of unknown species of microalgae, such as Phormidium species have been studied. Three solvents of different polarities (i.e., hexane, ethanol, and water) have been used to obtain pressurized liquid extracts with different compositions. Moreover, extractions were performed at four different extraction temperatures (50, 100, 150, and 200°C) with 20 min as extraction time. Antioxidant activity of the extracts has been measured by the TEAC assay. In general, hexane and ethanol extracts showed a higher antioxidant capacity that was mainly attributed to carotenoid compounds, as the TEAC value trend seems to be similar to the carotenoid content of... (More)

In the search for new functional ingredients with potential use in the food industry, extracts of unknown species of microalgae, such as Phormidium species have been studied. Three solvents of different polarities (i.e., hexane, ethanol, and water) have been used to obtain pressurized liquid extracts with different compositions. Moreover, extractions were performed at four different extraction temperatures (50, 100, 150, and 200°C) with 20 min as extraction time. Antioxidant activity of the extracts has been measured by the TEAC assay. In general, hexane and ethanol extracts showed a higher antioxidant capacity that was mainly attributed to carotenoid compounds, as the TEAC value trend seems to be similar to the carotenoid content of the extracts. On the other hand, the high antioxidant activity of the 200°C water extracts is likely related to the presence of Maillard reaction compounds produced by thermal degradation of the sample. β-Carotene, lutein, violaxanthin, and neoxanthin were identified in 150°C ethanol extracts. Four different microbial species (Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger) were used to screen the potential antimicrobial activity of the Phormidium sp. extracts. The most sensitive microorganism was the yeast, C. albicans, whereas the fungus, A. niger, was the most resistant. In general, no drastic differences were found for solvents and temperatures tested, showing a very diverse nature of the compounds responsible for the antimicrobial activity of these microalgae. In ethanol extracts, antimicrobial activity could be mainly attributed to the presence of terpenes (i.e., β-ionone, neophytadiene) and fatty acids (i.e., palmitoleic and linoleic acids) in the samples. Toxicity studies carried out with the extracts evaluated in the present work showed a cellular toxicity lower than those of other cyanobacteria such as Spirulina plantensis.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Antimicrobial activity, Antioxidant activity, Phormidium sp., Pressurized liquid extraction
in
Journal of Agricultural and Food Chemistry
volume
56
issue
10
pages
7 pages
publisher
The American Chemical Society
external identifiers
  • scopus:45549106586
ISSN
0021-8561
DOI
10.1021/jf703719p
language
English
LU publication?
no
id
6b26de9c-f3d4-4f55-85e8-63b5f3bb5f99
date added to LUP
2017-04-10 10:37:32
date last changed
2017-11-19 04:39:09
@article{6b26de9c-f3d4-4f55-85e8-63b5f3bb5f99,
  abstract     = {<p>In the search for new functional ingredients with potential use in the food industry, extracts of unknown species of microalgae, such as Phormidium species have been studied. Three solvents of different polarities (i.e., hexane, ethanol, and water) have been used to obtain pressurized liquid extracts with different compositions. Moreover, extractions were performed at four different extraction temperatures (50, 100, 150, and 200°C) with 20 min as extraction time. Antioxidant activity of the extracts has been measured by the TEAC assay. In general, hexane and ethanol extracts showed a higher antioxidant capacity that was mainly attributed to carotenoid compounds, as the TEAC value trend seems to be similar to the carotenoid content of the extracts. On the other hand, the high antioxidant activity of the 200°C water extracts is likely related to the presence of Maillard reaction compounds produced by thermal degradation of the sample. β-Carotene, lutein, violaxanthin, and neoxanthin were identified in 150°C ethanol extracts. Four different microbial species (Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger) were used to screen the potential antimicrobial activity of the Phormidium sp. extracts. The most sensitive microorganism was the yeast, C. albicans, whereas the fungus, A. niger, was the most resistant. In general, no drastic differences were found for solvents and temperatures tested, showing a very diverse nature of the compounds responsible for the antimicrobial activity of these microalgae. In ethanol extracts, antimicrobial activity could be mainly attributed to the presence of terpenes (i.e., β-ionone, neophytadiene) and fatty acids (i.e., palmitoleic and linoleic acids) in the samples. Toxicity studies carried out with the extracts evaluated in the present work showed a cellular toxicity lower than those of other cyanobacteria such as Spirulina plantensis.</p>},
  author       = {Rodríguez-Meizoso, I. and Jaime, Laura and Santoyo, Susana and Cifuentes, Alejandro and García-Blairsy Reina, G. and Señoráns, F. J. and Ibáñez, Elena},
  issn         = {0021-8561},
  keyword      = {Antimicrobial activity,Antioxidant activity,Phormidium sp.,Pressurized liquid extraction},
  language     = {eng},
  month        = {05},
  number       = {10},
  pages        = {3517--3523},
  publisher    = {The American Chemical Society},
  series       = {Journal of Agricultural and Food Chemistry},
  title        = {Pressurized fluid extraction of bioactive compounds from Phormidium species},
  url          = {http://dx.doi.org/10.1021/jf703719p},
  volume       = {56},
  year         = {2008},
}