Label-free acoustophoretic enrichment of mononuclear cells from blood
Urbansky, Anke LU ; Ohlsson, Pelle LU
; Lenshof, Andreas
LU
; Scheding, Stefan
LU
and Laurell, Thomas
LU
(2017)
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
In MicroTAS proceedings
2017.
p.1309-1310
- Abstract
We are reporting an efficient, label-free and continuous separation of mononuclear cells (MNC), from blood using microchip acoustophoresis. In standard PBS buffer, MNCs and red blood cells (RBC) display overlapping acoustophoretic mobilities which compromise separation of these cell types from each other. In this paper we capitalize on the fact that MNC and RBC display different acoustophysical properties. By optimizing the buffer conditions and thereby changing the acoustic contrast factor, and hence the acoustophoretic mobility of the cells, a 2800-fold enrichment of MNCs vs. RBCs with MNC recoveries up to 88% was accomplished.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/6c4f34bc-4543-4cdd-93bd-41b774616e5a
- author
- Urbansky, Anke
LU
; Ohlsson, Pelle
LU
; Lenshof, Andreas
LU
; Scheding, Stefan
LU
and Laurell, Thomas
LU
- organization
-
- Acoustofluidics group (research group)
- Department of Biomedical Engineering
- NanoLund: Centre for Nanoscience
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- Bone marrow stem cells and cellular therapies (research group)
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- publishing date
- 2017
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Acoustophoresis, Cell Separation, Label-free, Mononuclear Cells, Red Blood Cells
- host publication
- MicroTAS 2017 : Savannah, Georgia, USA - Savannah, Georgia, USA
- series title
- MicroTAS proceedings
- volume
- 2017
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
- conference location
- Savannah, United States
- conference dates
- 2017-10-22 - 2017-10-26
- external identifiers
-
- scopus:85079676224
- ISSN
- 1556-5904
- ISBN
- 9780692941836
- language
- English
- LU publication?
- yes
- id
- 6c4f34bc-4543-4cdd-93bd-41b774616e5a
- date added to LUP
- 2020-03-05 09:44:54
- date last changed
- 2023-11-05 13:45:03
@inproceedings{6c4f34bc-4543-4cdd-93bd-41b774616e5a,
abstract = {{<p>We are reporting an efficient, label-free and continuous separation of mononuclear cells (MNC), from blood using microchip acoustophoresis. In standard PBS buffer, MNCs and red blood cells (RBC) display overlapping acoustophoretic mobilities which compromise separation of these cell types from each other. In this paper we capitalize on the fact that MNC and RBC display different acoustophysical properties. By optimizing the buffer conditions and thereby changing the acoustic contrast factor, and hence the acoustophoretic mobility of the cells, a 2800-fold enrichment of MNCs vs. RBCs with MNC recoveries up to 88% was accomplished.</p>}},
author = {{Urbansky, Anke and Ohlsson, Pelle and Lenshof, Andreas and Scheding, Stefan and Laurell, Thomas}},
booktitle = {{MicroTAS 2017 : Savannah, Georgia, USA}},
isbn = {{9780692941836}},
issn = {{1556-5904}},
keywords = {{Acoustophoresis; Cell Separation; Label-free; Mononuclear Cells; Red Blood Cells}},
language = {{eng}},
pages = {{1309--1310}},
publisher = {{Chemical and Biological Microsystems Society}},
series = {{MicroTAS proceedings}},
title = {{Label-free acoustophoretic enrichment of mononuclear cells from blood}},
volume = {{2017}},
year = {{2017}},
}