Microfluidics-based approaches to the isolation of African trypanosomes
Barrett, Michael P. ; Cooper, Jonathan M. ; Regnault, Clément ; Holm, Stefan H. LU ; Beech, Jason P. LU ; Tegenfeldt, Jonas O. LU
and Hochstetter, Axel
(2017)
In Pathogens
6(4).
- Abstract
African trypanosomes are responsible for significant levels of disease in both humans and animals. The protozoan parasites are free-living flagellates, usually transmitted by arthropod vectors, including the tsetse fly. In the mammalian host they live in the bloodstream and, in the case of human-infectious species, later invade the central nervous system. Diagnosis of the disease requires the positive identification of parasites in the bloodstream. This can be particularly challenging where parasite numbers are low, as is often the case in peripheral blood. Enriching parasites from body fluids is an important part of the diagnostic pathway. As more is learned about the physicochemical properties of trypanosomes, this information can be... (More)
African trypanosomes are responsible for significant levels of disease in both humans and animals. The protozoan parasites are free-living flagellates, usually transmitted by arthropod vectors, including the tsetse fly. In the mammalian host they live in the bloodstream and, in the case of human-infectious species, later invade the central nervous system. Diagnosis of the disease requires the positive identification of parasites in the bloodstream. This can be particularly challenging where parasite numbers are low, as is often the case in peripheral blood. Enriching parasites from body fluids is an important part of the diagnostic pathway. As more is learned about the physicochemical properties of trypanosomes, this information can be exploited through use of different microfluidic-based approaches to isolate the parasites from blood or other fluids. Here, we discuss recent advances in the use of microfluidics to separate trypanosomes from blood and to isolate single trypanosomes for analyses including drug screening.
(Less)
- author
- Barrett, Michael P.
; Cooper, Jonathan M.
; Regnault, Clément
; Holm, Stefan H.
LU
; Beech, Jason P.
LU
; Tegenfeldt, Jonas O.
LU
and Hochstetter, Axel
- organization
- publishing date
- 2017-12-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Concentration ramping, Diagnosis, Dielectrophoresis, DLD, Drug testing, Isolation, Microfluidics, Optical trap, Separation, Trypanosomes
- in
- Pathogens
- volume
- 6
- issue
- 4
- article number
- 7
- publisher
- MDPI AG
- external identifiers
-
- scopus:85032854907
- pmid:28981471
- wos:000417347700003
- ISSN
- 2076-0817
- DOI
- 10.3390/pathogens6040047
- language
- English
- LU publication?
- yes
- id
- 6c77b847-6a78-4be2-b9e7-b140c5882a6c
- date added to LUP
- 2017-11-22 08:39:27
- date last changed
- 2024-06-11 06:41:30
@article{6c77b847-6a78-4be2-b9e7-b140c5882a6c,
abstract = {{<p>African trypanosomes are responsible for significant levels of disease in both humans and animals. The protozoan parasites are free-living flagellates, usually transmitted by arthropod vectors, including the tsetse fly. In the mammalian host they live in the bloodstream and, in the case of human-infectious species, later invade the central nervous system. Diagnosis of the disease requires the positive identification of parasites in the bloodstream. This can be particularly challenging where parasite numbers are low, as is often the case in peripheral blood. Enriching parasites from body fluids is an important part of the diagnostic pathway. As more is learned about the physicochemical properties of trypanosomes, this information can be exploited through use of different microfluidic-based approaches to isolate the parasites from blood or other fluids. Here, we discuss recent advances in the use of microfluidics to separate trypanosomes from blood and to isolate single trypanosomes for analyses including drug screening.</p>}},
author = {{Barrett, Michael P. and Cooper, Jonathan M. and Regnault, Clément and Holm, Stefan H. and Beech, Jason P. and Tegenfeldt, Jonas O. and Hochstetter, Axel}},
issn = {{2076-0817}},
keywords = {{Concentration ramping; Diagnosis; Dielectrophoresis; DLD; Drug testing; Isolation; Microfluidics; Optical trap; Separation; Trypanosomes}},
language = {{eng}},
month = {{12}},
number = {{4}},
publisher = {{MDPI AG}},
series = {{Pathogens}},
title = {{Microfluidics-based approaches to the isolation of African trypanosomes}},
url = {{http://dx.doi.org/10.3390/pathogens6040047}},
doi = {{10.3390/pathogens6040047}},
volume = {{6}},
year = {{2017}},
}