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Dynamics of highly concentrated protein solutions around the denaturing transition

Hennig, Marcus ; Roosen-Runge, Felix LU ; Zhang, Fajun ; Zorn, Stefan ; Skoda, Maximilian W A ; Jacobs, Robert M J ; Seydel, Tilo and Schreiber, Frank (2012) In Soft Matter 8(5). p.1628-1633
Abstract

Using both quasi-elastic and fixed-window neutron spectroscopy, we study the dynamics of highly concentrated aqueous protein solutions of bovine serum albumin around the denaturing transition. For the temperature range 280 K < T < 370 K, the total mean-squared displacement 〈u 2〉 is recorded. Below and above the denaturing, we observe that 〈u 2〉 increases monotonically with T, but at the denaturing transition it decreases strongly. This observation can be rationalized and quantitatively modeled as a transition from a liquid protein solution to a gel-like state. Atomic vibrations, molecular subunit diffusion and, most importantly, diffusion of the entire protein determine 〈u 2〉. The latter is... (More)

Using both quasi-elastic and fixed-window neutron spectroscopy, we study the dynamics of highly concentrated aqueous protein solutions of bovine serum albumin around the denaturing transition. For the temperature range 280 K < T < 370 K, the total mean-squared displacement 〈u 2〉 is recorded. Below and above the denaturing, we observe that 〈u 2〉 increases monotonically with T, but at the denaturing transition it decreases strongly. This observation can be rationalized and quantitatively modeled as a transition from a liquid protein solution to a gel-like state. Atomic vibrations, molecular subunit diffusion and, most importantly, diffusion of the entire protein determine 〈u 2〉. The latter is strongly hindered due to entanglement and cross-linking of the chains and causes the pronounced decrease of 〈u 2〉. Using information from the full quasi-elastic signal, we separate the diffusion contribution from 〈u 2〉 and reveal the transition temperature. For the analysis of this separation, we introduce a general concept, which is applicable to other colloid systems exhibiting both center-of-mass and internal dynamics.

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author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Soft Matter
volume
8
issue
5
pages
6 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:84862952526
ISSN
1744-683X
DOI
10.1039/c1sm06609a
language
English
LU publication?
no
id
6c9c78d1-75d3-44e9-b08f-33cfc49a80c7
date added to LUP
2018-12-17 09:51:42
date last changed
2022-01-31 07:54:54
@article{6c9c78d1-75d3-44e9-b08f-33cfc49a80c7,
  abstract     = {{<p>Using both quasi-elastic and fixed-window neutron spectroscopy, we study the dynamics of highly concentrated aqueous protein solutions of bovine serum albumin around the denaturing transition. For the temperature range 280 K &lt; T &lt; 370 K, the total mean-squared displacement 〈u <sup>2</sup>〉 is recorded. Below and above the denaturing, we observe that 〈u <sup>2</sup>〉 increases monotonically with T, but at the denaturing transition it decreases strongly. This observation can be rationalized and quantitatively modeled as a transition from a liquid protein solution to a gel-like state. Atomic vibrations, molecular subunit diffusion and, most importantly, diffusion of the entire protein determine 〈u <sup>2</sup>〉. The latter is strongly hindered due to entanglement and cross-linking of the chains and causes the pronounced decrease of 〈u <sup>2</sup>〉. Using information from the full quasi-elastic signal, we separate the diffusion contribution from 〈u <sup>2</sup>〉 and reveal the transition temperature. For the analysis of this separation, we introduce a general concept, which is applicable to other colloid systems exhibiting both center-of-mass and internal dynamics.</p>}},
  author       = {{Hennig, Marcus and Roosen-Runge, Felix and Zhang, Fajun and Zorn, Stefan and Skoda, Maximilian W A and Jacobs, Robert M J and Seydel, Tilo and Schreiber, Frank}},
  issn         = {{1744-683X}},
  language     = {{eng}},
  month        = {{02}},
  number       = {{5}},
  pages        = {{1628--1633}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Soft Matter}},
  title        = {{Dynamics of highly concentrated protein solutions around the denaturing transition}},
  url          = {{http://dx.doi.org/10.1039/c1sm06609a}},
  doi          = {{10.1039/c1sm06609a}},
  volume       = {{8}},
  year         = {{2012}},
}