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Mg(2+) signalling defines the group A streptococcal CsrRS (CovRS) regulon

Gryllos, Ioannis; Grifantini, Renata; Colaprico, Annalisa; Jiang, Shengmei; Deforce, Emelia; Hakansson, Anders LU ; Telford, John L; Grandi, Guido and Wessels, Michael R (2007) In Molecular Microbiology 65(3). p.671-683
Abstract

CsrRS (or CovRS) is a two-component system implicated in the control of multiple virulence determinants in the important human pathogen, group A Streptococcus (GAS). Earlier studies suggested that extracellular Mg(2+) signalled through the presumed sensor histidine kinase, CsrS. We now confirm those findings, as complementation of a csrS mutant restored Mg(2+)-dependent gene regulation. Moreover, we present strong evidence that Mg(2+) signals through CsrS to regulate an extensive and previously undefined repertoire of GAS genes. The effect of Mg(2+) on regulation of global gene expression was evaluated using genomic microarrays in an M-type 3 strain of GAS and in an isogenic csrS mutant. Unexpectedly, of the 72 genes identified in the... (More)

CsrRS (or CovRS) is a two-component system implicated in the control of multiple virulence determinants in the important human pathogen, group A Streptococcus (GAS). Earlier studies suggested that extracellular Mg(2+) signalled through the presumed sensor histidine kinase, CsrS. We now confirm those findings, as complementation of a csrS mutant restored Mg(2+)-dependent gene regulation. Moreover, we present strong evidence that Mg(2+) signals through CsrS to regulate an extensive and previously undefined repertoire of GAS genes. The effect of Mg(2+) on regulation of global gene expression was evaluated using genomic microarrays in an M-type 3 strain of GAS and in an isogenic csrS mutant. Unexpectedly, of the 72 genes identified in the Mg(2+)-stimulated CsrRS regulon, 42 were absent from the CsrR regulon (the latter being defined by comparison of wild-type and CsrR mutant transcriptomes at low Mg(2+)). We observed CsrS-dependent regulation of 72 of the 73 genes whose expression changed in response to elevated extracellular Mg(2+) in wild-type bacteria, a result that identifies CsrS as the principal, if not exclusive, sensor for extracellular Mg(2+) in GAS. To our knowledge, this study is the first to characterize global gene regulation by a GAS two-component system in response to a specific environmental stimulus.

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author
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Contribution to journal
publication status
published
subject
keywords
Bacterial Proteins, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Genes, Bacterial, Genetic Complementation Test, Magnesium, Mutation, Oligonucleotide Array Sequence Analysis, Plasmids, Protein Kinases, Regulon, Repressor Proteins, Reproducibility of Results, Signal Transduction, Streptococcus pyogenes
in
Molecular Microbiology
volume
65
issue
3
pages
13 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:34447573810
ISSN
0950-382X
DOI
10.1111/j.1365-2958.2007.05818.x
language
English
LU publication?
no
id
6cb45586-166e-4ecb-a1cf-182f00c8b4a8
date added to LUP
2016-05-21 13:52:36
date last changed
2017-09-17 09:15:25
@article{6cb45586-166e-4ecb-a1cf-182f00c8b4a8,
  abstract     = {<p>CsrRS (or CovRS) is a two-component system implicated in the control of multiple virulence determinants in the important human pathogen, group A Streptococcus (GAS). Earlier studies suggested that extracellular Mg(2+) signalled through the presumed sensor histidine kinase, CsrS. We now confirm those findings, as complementation of a csrS mutant restored Mg(2+)-dependent gene regulation. Moreover, we present strong evidence that Mg(2+) signals through CsrS to regulate an extensive and previously undefined repertoire of GAS genes. The effect of Mg(2+) on regulation of global gene expression was evaluated using genomic microarrays in an M-type 3 strain of GAS and in an isogenic csrS mutant. Unexpectedly, of the 72 genes identified in the Mg(2+)-stimulated CsrRS regulon, 42 were absent from the CsrR regulon (the latter being defined by comparison of wild-type and CsrR mutant transcriptomes at low Mg(2+)). We observed CsrS-dependent regulation of 72 of the 73 genes whose expression changed in response to elevated extracellular Mg(2+) in wild-type bacteria, a result that identifies CsrS as the principal, if not exclusive, sensor for extracellular Mg(2+) in GAS. To our knowledge, this study is the first to characterize global gene regulation by a GAS two-component system in response to a specific environmental stimulus.</p>},
  author       = {Gryllos, Ioannis and Grifantini, Renata and Colaprico, Annalisa and Jiang, Shengmei and Deforce, Emelia and Hakansson, Anders and Telford, John L and Grandi, Guido and Wessels, Michael R},
  issn         = {0950-382X},
  keyword      = {Bacterial Proteins,Gene Expression Profiling,Gene Expression Regulation, Bacterial,Genes, Bacterial,Genetic Complementation Test,Magnesium,Mutation,Oligonucleotide Array Sequence Analysis,Plasmids,Protein Kinases,Regulon,Repressor Proteins,Reproducibility of Results,Signal Transduction,Streptococcus pyogenes},
  language     = {eng},
  number       = {3},
  pages        = {671--683},
  publisher    = {Wiley-Blackwell},
  series       = {Molecular Microbiology},
  title        = {Mg(2+) signalling defines the group A streptococcal CsrRS (CovRS) regulon},
  url          = {http://dx.doi.org/10.1111/j.1365-2958.2007.05818.x},
  volume       = {65},
  year         = {2007},
}