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Fatty acid selectivity of a lipase purified from Vernonia galamensis seed

Ncube, Ignatious ; Gitlesen, Thomas ; Adlercreutz, Patrick LU ; Read, John S. and Mattiasson, Bo LU (1995) In Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism 1257(2). p.149-156
Abstract

Vernonia galamensis is an annual herb whose seed oil contains high levels of an epoxy fatty acid, vernolic (cis-12,13-epoxy cis-9-octadecenoic) acid. The seed also contains lipase activity in the dormant state. A lipase was purified from the seed and its substrate specificity studied in isooctane. The lipase shows pronounced selectivity for the native triacylglycerol, trivernolin. The rate of hydrolysis of triolein, the corresponding non epoxy triacylglycerol, is only 3% of that of trivernolin. In the acidolysis of tricaprylin using a mixture of fatty acids, the Vernonia lipase also showed selectivity for vernolic acid. Michaelis-Menten kinetics of the hydrolysis of triacylglycerols revealed that the observed high selectivity of the... (More)

Vernonia galamensis is an annual herb whose seed oil contains high levels of an epoxy fatty acid, vernolic (cis-12,13-epoxy cis-9-octadecenoic) acid. The seed also contains lipase activity in the dormant state. A lipase was purified from the seed and its substrate specificity studied in isooctane. The lipase shows pronounced selectivity for the native triacylglycerol, trivernolin. The rate of hydrolysis of triolein, the corresponding non epoxy triacylglycerol, is only 3% of that of trivernolin. In the acidolysis of tricaprylin using a mixture of fatty acids, the Vernonia lipase also showed selectivity for vernolic acid. Michaelis-Menten kinetics of the hydrolysis of triacylglycerols revealed that the observed high selectivity of the Vernonia lipase for trivernolin was mainly due to a higher Vmax for trivernolin. The Vmax value for the hydrolysis of trivernolin was 5 times higher than that for triolein. This novel substrate specificity is an adaptation by the seed lipase to the triacylglycerols of the seed oil that contain up to 80% vernolic acid.

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organization
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Contribution to journal
publication status
published
subject
keywords
(V. galamensis), Fatty acid selectivity, Lipase, Purification
in
Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
volume
1257
issue
2
pages
8 pages
publisher
Elsevier
external identifiers
  • pmid:7619855
  • scopus:0029059160
ISSN
0005-2760
DOI
10.1016/0005-2760(95)00067-M
language
English
LU publication?
yes
id
6cc7a6cf-85c7-42a2-ba93-b77489be3776
date added to LUP
2019-06-20 15:38:36
date last changed
2019-11-25 09:31:56
@article{6cc7a6cf-85c7-42a2-ba93-b77489be3776,
  abstract     = {<p>Vernonia galamensis is an annual herb whose seed oil contains high levels of an epoxy fatty acid, vernolic (cis-12,13-epoxy cis-9-octadecenoic) acid. The seed also contains lipase activity in the dormant state. A lipase was purified from the seed and its substrate specificity studied in isooctane. The lipase shows pronounced selectivity for the native triacylglycerol, trivernolin. The rate of hydrolysis of triolein, the corresponding non epoxy triacylglycerol, is only 3% of that of trivernolin. In the acidolysis of tricaprylin using a mixture of fatty acids, the Vernonia lipase also showed selectivity for vernolic acid. Michaelis-Menten kinetics of the hydrolysis of triacylglycerols revealed that the observed high selectivity of the Vernonia lipase for trivernolin was mainly due to a higher V<sub>max</sub> for trivernolin. The V<sub>max</sub> value for the hydrolysis of trivernolin was 5 times higher than that for triolein. This novel substrate specificity is an adaptation by the seed lipase to the triacylglycerols of the seed oil that contain up to 80% vernolic acid.</p>},
  author       = {Ncube, Ignatious and Gitlesen, Thomas and Adlercreutz, Patrick and Read, John S. and Mattiasson, Bo},
  issn         = {0005-2760},
  language     = {eng},
  month        = {07},
  number       = {2},
  pages        = {149--156},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism},
  title        = {Fatty acid selectivity of a lipase purified from Vernonia galamensis seed},
  url          = {http://dx.doi.org/10.1016/0005-2760(95)00067-M},
  doi          = {10.1016/0005-2760(95)00067-M},
  volume       = {1257},
  year         = {1995},
}