Derivation of Adult Human Cortical Organotypic Slice Cultures for Coculture with Reprogrammed Neuronal Cells
(2021) In Methods in Molecular Biology 2352. p.253-259- Abstract
Adult human cortical organotypic slice culture is an attractive model system to explore mechanisms of human brain pathology as well as to test drug candidates for treatment of neurodegeneration. Acute studies in human brain slices are limited by the lifetime of the tissue and focus mainly on hippocampus slice preparation. Here we describe the derivation of human organotypic slice cultures of cortical origin, which can be kept in culture for up to 6 weeks. This method enabled us to test the system in coculture with reprogrammed neurons and show its feasibility in neuronal cell integration experiments in human-to-human grafting situation.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/6cc9d8e4-1170-4137-9784-18b1713a9ddf
- author
- Kvist, Giedre LU
- organization
- publishing date
- 2021
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Brain, Human cortex, Neurodegeneration, Organotypic slice cultures, Reprogrammed neurons, Vibratome
- host publication
- Neural Reprogramming
- series title
- Methods in Molecular Biology
- volume
- 2352
- pages
- 253 - 259
- publisher
- Humana Press
- external identifiers
-
- scopus:85111865365
- pmid:34324192
- ISSN
- 1064-3745
- 1940-6029
- ISBN
- 978-1-0716-1601-7
- 978-1-0716-1600-0
- DOI
- 10.1007/978-1-0716-1601-7_17
- language
- English
- LU publication?
- yes
- id
- 6cc9d8e4-1170-4137-9784-18b1713a9ddf
- date added to LUP
- 2021-12-10 09:44:08
- date last changed
- 2024-09-22 07:25:36
@inbook{6cc9d8e4-1170-4137-9784-18b1713a9ddf,
abstract = {{<p>Adult human cortical organotypic slice culture is an attractive model system to explore mechanisms of human brain pathology as well as to test drug candidates for treatment of neurodegeneration. Acute studies in human brain slices are limited by the lifetime of the tissue and focus mainly on hippocampus slice preparation. Here we describe the derivation of human organotypic slice cultures of cortical origin, which can be kept in culture for up to 6 weeks. This method enabled us to test the system in coculture with reprogrammed neurons and show its feasibility in neuronal cell integration experiments in human-to-human grafting situation.</p>}},
author = {{Kvist, Giedre}},
booktitle = {{Neural Reprogramming}},
isbn = {{978-1-0716-1601-7}},
issn = {{1064-3745}},
keywords = {{Brain; Human cortex; Neurodegeneration; Organotypic slice cultures; Reprogrammed neurons; Vibratome}},
language = {{eng}},
pages = {{253--259}},
publisher = {{Humana Press}},
series = {{Methods in Molecular Biology}},
title = {{Derivation of Adult Human Cortical Organotypic Slice Cultures for Coculture with Reprogrammed Neuronal Cells}},
url = {{http://dx.doi.org/10.1007/978-1-0716-1601-7_17}},
doi = {{10.1007/978-1-0716-1601-7_17}},
volume = {{2352}},
year = {{2021}},
}