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Multicenter prospective study of the humoral autoimmune response in bullous pemphigoid

Di Zenzo, Giovanni; Thoma-Uszynski, Sybille; Fontao, Lionel; Calabresi, Valentina; Hofmann, Silke C.; Hellmark, Thomas LU ; Sebbag, Nathalie; Pedicelli, Cristina; Sera, Francesco and Lacour, Jean Philippe, et al. (2008) In Clinical Immunology1999-01-01+01:00 128(3). p.415-426
Abstract

Bullous pemphigoid (BP) is an autoimmune bullous disease, associated with autoantibodies directed against the hemidesmosomal components BP180 and BP230. In this study for the first time different laboratories have analyzed the autoantibody profile in the same group of 49 prospectively recruited BP patients. The results show that: 1) disease severity and activity correlated with levels of IgG against the BP180-NC16A domain, but also against a COOH-terminal epitope of BP180, 2) distinct epitopes of the BP180 ectodomain other than BP180-NC16A were recognized by 96% of the BP sera; and 3) the combined use of BP180 and BP230 ELISA led to the detection of IgG autoantibodies in all the BP sera. These results demonstrate the usefulness of the... (More)

Bullous pemphigoid (BP) is an autoimmune bullous disease, associated with autoantibodies directed against the hemidesmosomal components BP180 and BP230. In this study for the first time different laboratories have analyzed the autoantibody profile in the same group of 49 prospectively recruited BP patients. The results show that: 1) disease severity and activity correlated with levels of IgG against the BP180-NC16A domain, but also against a COOH-terminal epitope of BP180, 2) distinct epitopes of the BP180 ectodomain other than BP180-NC16A were recognized by 96% of the BP sera; and 3) the combined use of BP180 and BP230 ELISA led to the detection of IgG autoantibodies in all the BP sera. These results demonstrate the usefulness of the combined ELISAs based on various BP180 and BP230 fragments in establishing the diagnosis of BP and support the concept that BP180 is the major autoantigen of BP.

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type
Contribution to journal
publication status
published
subject
keywords
Autoantibodies, BP180, BP230, Bullous pemphigoid, Epitope mapping
in
Clinical Immunology1999-01-01+01:00
volume
128
issue
3
pages
415 - 426
publisher
Elsevier
external identifiers
  • scopus:49149098929
ISSN
1521-6616
DOI
10.1016/j.clim.2008.04.012
language
English
LU publication?
no
id
6cead2ec-117c-4263-8174-4349f38c60e1
date added to LUP
2018-11-05 19:32:57
date last changed
2019-08-11 04:43:21
@article{6cead2ec-117c-4263-8174-4349f38c60e1,
  abstract     = {<p>Bullous pemphigoid (BP) is an autoimmune bullous disease, associated with autoantibodies directed against the hemidesmosomal components BP180 and BP230. In this study for the first time different laboratories have analyzed the autoantibody profile in the same group of 49 prospectively recruited BP patients. The results show that: 1) disease severity and activity correlated with levels of IgG against the BP180-NC16A domain, but also against a COOH-terminal epitope of BP180, 2) distinct epitopes of the BP180 ectodomain other than BP180-NC16A were recognized by 96% of the BP sera; and 3) the combined use of BP180 and BP230 ELISA led to the detection of IgG autoantibodies in all the BP sera. These results demonstrate the usefulness of the combined ELISAs based on various BP180 and BP230 fragments in establishing the diagnosis of BP and support the concept that BP180 is the major autoantigen of BP.</p>},
  author       = {Di Zenzo, Giovanni and Thoma-Uszynski, Sybille and Fontao, Lionel and Calabresi, Valentina and Hofmann, Silke C. and Hellmark, Thomas and Sebbag, Nathalie and Pedicelli, Cristina and Sera, Francesco and Lacour, Jean Philippe and Wieslander, Jörgen and Bruckner-Tuderman, Leena and Borradori, Luca and Zambruno, Giovanna and Hertl, Michael},
  issn         = {1521-6616},
  keyword      = {Autoantibodies,BP180,BP230,Bullous pemphigoid,Epitope mapping},
  language     = {eng},
  number       = {3},
  pages        = {415--426},
  publisher    = {Elsevier},
  series       = {Clinical Immunology1999-01-01+01:00},
  title        = {Multicenter prospective study of the humoral autoimmune response in bullous pemphigoid},
  url          = {http://dx.doi.org/10.1016/j.clim.2008.04.012},
  volume       = {128},
  year         = {2008},
}