Freeze-dried and re-hydrated liquid crystalline nanoparticles stabilized with disaccharides for drug-delivery of the plectasin derivative AP114 antimicrobial peptide
(2018) In Journal of Colloid and Interface Science 522. p.126-135- Abstract
Liquid crystalline nanoparticles (LCNPs), e.g. cubosomes and hexosomes, are receiving more and more attraction as drug delivery vehicles. Dry powder formulation that forms LCNPs upon hydration can be advantageous to make new routes of administration accessible. In this work, we investigate use of three disaccharides (lactose, trehalose and sucrose) as protective matrices for glycerol monooleate based LCNP forming powders produced by freeze-drying. Phase behavior, particle size and size distributions at the different preparation steps were monitored by small angle x-ray scattering (SAXS) and dynamic light scattering (DLS). Particle appearance was imaged by cryogenic transmission electron microscopy (cryo-TEM). Moreover, the therapeutic... (More)
Liquid crystalline nanoparticles (LCNPs), e.g. cubosomes and hexosomes, are receiving more and more attraction as drug delivery vehicles. Dry powder formulation that forms LCNPs upon hydration can be advantageous to make new routes of administration accessible. In this work, we investigate use of three disaccharides (lactose, trehalose and sucrose) as protective matrices for glycerol monooleate based LCNP forming powders produced by freeze-drying. Phase behavior, particle size and size distributions at the different preparation steps were monitored by small angle x-ray scattering (SAXS) and dynamic light scattering (DLS). Particle appearance was imaged by cryogenic transmission electron microscopy (cryo-TEM). Moreover, the therapeutic relevant antimicrobial peptide AP114 (plectasin derivative) was incorporated in the formulations. Peptide encapsulation and release as well as in vitro antibacterial effect were investigated. Results showed that all freeze-dried powders did form particles with liquid crystalline structure upon hydration. However, a phase transition from the bicontinuous cubic Pn3m to the reversed hexagonal was observed, as a consequence of sugar addition and the freeze-drying procedure. Data indicates that trehalose is the preferred choice of lyo-protectant in order to maintain a mono-modal particle size distribution. In addition, antimicrobial activity of AP114-containing formulations was found to be highest for the formulation containing trehalose. The release kinetics of AP114 from the nanoparticles was strongly affected by the dimensions of the hexagonal phase. Larger dimension of the hexagonal phase, significantly improved the release of AP114 and antimicrobial activity of the formulation.
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- author
- Boge, Lukas ; Västberg, Amanda LU ; Umerska, Anita ; Bysell, Helena ; Eriksson, Jonny ; Edwards, Katarina ; Millqvist-Fureby, Anna LU and Andersson, Martin
- publishing date
- 2018-07-15
- type
- Contribution to journal
- publication status
- published
- keywords
- Antimicrobial peptide, AP114, Cubosome, Freeze-drying, Glycerol monooleate, Hexosome, Liquid crystal, Plectasin
- in
- Journal of Colloid and Interface Science
- volume
- 522
- pages
- 10 pages
- publisher
- Academic Press
- external identifiers
-
- pmid:29587194
- scopus:85044472786
- ISSN
- 0021-9797
- DOI
- 10.1016/j.jcis.2018.03.062
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2018 Elsevier Inc.
- id
- 6d75ec8e-ea42-4a38-ad3a-433de5adeeca
- date added to LUP
- 2025-04-02 22:12:09
- date last changed
- 2025-07-24 16:32:53
@article{6d75ec8e-ea42-4a38-ad3a-433de5adeeca,
abstract = {{<p>Liquid crystalline nanoparticles (LCNPs), e.g. cubosomes and hexosomes, are receiving more and more attraction as drug delivery vehicles. Dry powder formulation that forms LCNPs upon hydration can be advantageous to make new routes of administration accessible. In this work, we investigate use of three disaccharides (lactose, trehalose and sucrose) as protective matrices for glycerol monooleate based LCNP forming powders produced by freeze-drying. Phase behavior, particle size and size distributions at the different preparation steps were monitored by small angle x-ray scattering (SAXS) and dynamic light scattering (DLS). Particle appearance was imaged by cryogenic transmission electron microscopy (cryo-TEM). Moreover, the therapeutic relevant antimicrobial peptide AP114 (plectasin derivative) was incorporated in the formulations. Peptide encapsulation and release as well as in vitro antibacterial effect were investigated. Results showed that all freeze-dried powders did form particles with liquid crystalline structure upon hydration. However, a phase transition from the bicontinuous cubic Pn3m to the reversed hexagonal was observed, as a consequence of sugar addition and the freeze-drying procedure. Data indicates that trehalose is the preferred choice of lyo-protectant in order to maintain a mono-modal particle size distribution. In addition, antimicrobial activity of AP114-containing formulations was found to be highest for the formulation containing trehalose. The release kinetics of AP114 from the nanoparticles was strongly affected by the dimensions of the hexagonal phase. Larger dimension of the hexagonal phase, significantly improved the release of AP114 and antimicrobial activity of the formulation.</p>}},
author = {{Boge, Lukas and Västberg, Amanda and Umerska, Anita and Bysell, Helena and Eriksson, Jonny and Edwards, Katarina and Millqvist-Fureby, Anna and Andersson, Martin}},
issn = {{0021-9797}},
keywords = {{Antimicrobial peptide; AP114; Cubosome; Freeze-drying; Glycerol monooleate; Hexosome; Liquid crystal; Plectasin}},
language = {{eng}},
month = {{07}},
pages = {{126--135}},
publisher = {{Academic Press}},
series = {{Journal of Colloid and Interface Science}},
title = {{Freeze-dried and re-hydrated liquid crystalline nanoparticles stabilized with disaccharides for drug-delivery of the plectasin derivative AP114 antimicrobial peptide}},
url = {{http://dx.doi.org/10.1016/j.jcis.2018.03.062}},
doi = {{10.1016/j.jcis.2018.03.062}},
volume = {{522}},
year = {{2018}},
}