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Distinct Microbial and Host Proteome Signatures Relate to Proteolytic Activity in Gingival Crevicular Fluid in an Exploratory Study of Patients With Periodontitis

Carda-Diéguez, Miguel ; Rüdiger, Stefan ; Svensäter, Gunnel ; Jansson, Henrik LU ; Mohanty, Tirthankar LU ; Chao, Yashuan LU ; Karlsson, Christofer LU ; Malmström, Johan LU orcid ; Shannon, Oonagh LU and Mira, Álex , et al. (2026) In Journal of Clinical Periodontology p.1-11
Abstract

AIM: Risk assessment tools for periodontitis lack sufficient precision to predict disease progression. The aim of this exploratory study was to evaluate proteolytic activity (PA) in gingival crevicular fluid in relation to clinically assessed progression risk, microbial community composition and host proteome.

MATERIALS AND METHODS: Samples were collected from 109 patients with periodontitis and stratified into high and low PA groups using a fluorescent substrate. Microbial composition was analysed via 16S rRNA gene sequencing, and host proteomic profiles were determined using mass spectrometry.

RESULTS: Forty-one of 42 patients in the high PA group were clinically classified as high-risk, while the low-activity group... (More)

AIM: Risk assessment tools for periodontitis lack sufficient precision to predict disease progression. The aim of this exploratory study was to evaluate proteolytic activity (PA) in gingival crevicular fluid in relation to clinically assessed progression risk, microbial community composition and host proteome.

MATERIALS AND METHODS: Samples were collected from 109 patients with periodontitis and stratified into high and low PA groups using a fluorescent substrate. Microbial composition was analysed via 16S rRNA gene sequencing, and host proteomic profiles were determined using mass spectrometry.

RESULTS: Forty-one of 42 patients in the high PA group were clinically classified as high-risk, while the low-activity group included both low- and high-risk individuals. High-activity samples displayed greater microbial diversity and enrichment of proteolytic species including Fusobacterium nucleatum, Porphyromonas gingivalis and Treponema denticola. Of the 235 differentially expressed host proteins, high-activity samples had elevated markers of inflammation and neutrophil-mediated immunity, whereas low-activity samples were enriched in protease inhibitors, indicating more effective regulation of tissue degradation.

CONCLUSIONS: Small-volume gingival crevicular fluid sampling allows integrated analysis of microbial and host signatures that provide insight into the pathobiology of periodontitis. PA stratifies patients according to these microbial and host proteome signatures.

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organization
publishing date
type
Contribution to journal
publication status
epub
subject
in
Journal of Clinical Periodontology
pages
1 - 11
publisher
Wiley-Blackwell
external identifiers
  • pmid:42634008
ISSN
1600-051X
DOI
10.1111/jcpe.70186
language
English
LU publication?
yes
additional info
© 2026 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
id
6e05052a-a794-4b89-8c25-2b0f6d53e882
date added to LUP
2026-08-27 06:19:27
date last changed
2026-08-27 09:24:57
@article{6e05052a-a794-4b89-8c25-2b0f6d53e882,
  abstract     = {{<p>AIM: Risk assessment tools for periodontitis lack sufficient precision to predict disease progression. The aim of this exploratory study was to evaluate proteolytic activity (PA) in gingival crevicular fluid in relation to clinically assessed progression risk, microbial community composition and host proteome.</p><p>MATERIALS AND METHODS: Samples were collected from 109 patients with periodontitis and stratified into high and low PA groups using a fluorescent substrate. Microbial composition was analysed via 16S rRNA gene sequencing, and host proteomic profiles were determined using mass spectrometry.</p><p>RESULTS: Forty-one of 42 patients in the high PA group were clinically classified as high-risk, while the low-activity group included both low- and high-risk individuals. High-activity samples displayed greater microbial diversity and enrichment of proteolytic species including Fusobacterium nucleatum, Porphyromonas gingivalis and Treponema denticola. Of the 235 differentially expressed host proteins, high-activity samples had elevated markers of inflammation and neutrophil-mediated immunity, whereas low-activity samples were enriched in protease inhibitors, indicating more effective regulation of tissue degradation.</p><p>CONCLUSIONS: Small-volume gingival crevicular fluid sampling allows integrated analysis of microbial and host signatures that provide insight into the pathobiology of periodontitis. PA stratifies patients according to these microbial and host proteome signatures.</p>}},
  author       = {{Carda-Diéguez, Miguel and Rüdiger, Stefan and Svensäter, Gunnel and Jansson, Henrik and Mohanty, Tirthankar and Chao, Yashuan and Karlsson, Christofer and Malmström, Johan and Shannon, Oonagh and Mira, Álex and Davies, Julia R}},
  issn         = {{1600-051X}},
  language     = {{eng}},
  month        = {{08}},
  pages        = {{1--11}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Journal of Clinical Periodontology}},
  title        = {{Distinct Microbial and Host Proteome Signatures Relate to Proteolytic Activity in Gingival Crevicular Fluid in an Exploratory Study of Patients With Periodontitis}},
  url          = {{http://dx.doi.org/10.1111/jcpe.70186}},
  doi          = {{10.1111/jcpe.70186}},
  year         = {{2026}},
}