Advanced

Evolution of Bioengineered Lung Models : Recent Advances and Challenges in Tissue Mimicry for Studying the Role of Mechanical Forces in Cell Biology

Doryab, Ali; Tas, Sinem LU ; Taskin, Mehmet Berat; Yang, Lin; Hilgendorff, Anne; Groll, Jürgen; Wagner, Darcy E. LU and Schmid, Otmar (2019) In Advanced Functional Materials
Abstract

Mechanical stretch under both physiological (breathing) and pathophysiological (ventilator-induced) conditions is known to significantly impact all cellular compartments in the lung, thereby playing a pivotal role in lung growth, regeneration and disease development. In order to evaluate the impact of mechanical forces on the cellular level, in vitro models using lung cells on stretchable membranes have been developed. Only recently have some of these cell-stretching devices become suitable for air–liquid interface cell cultures, which is required to adequately model physiological conditions for the alveolar epithelium. To reach this goal, a multi-functional membrane for cell growth balancing biophysical and mechanical properties is... (More)

Mechanical stretch under both physiological (breathing) and pathophysiological (ventilator-induced) conditions is known to significantly impact all cellular compartments in the lung, thereby playing a pivotal role in lung growth, regeneration and disease development. In order to evaluate the impact of mechanical forces on the cellular level, in vitro models using lung cells on stretchable membranes have been developed. Only recently have some of these cell-stretching devices become suitable for air–liquid interface cell cultures, which is required to adequately model physiological conditions for the alveolar epithelium. To reach this goal, a multi-functional membrane for cell growth balancing biophysical and mechanical properties is critical to mimic (patho)physiological conditions. In this review, i) the relevance of cyclic mechanical forces in lung biology is elucidated, ii) the physiological range for the key parameters of tissue stretch in the lung is described, and iii) the currently available in vitro cell-stretching devices are discussed. After assessing various polymers, it is concluded that natural-synthetic copolymers are promising candidates for suitable stretchable membranes used in cell-stretching models. This work provides guidance on future developments in biomimetic in vitro models of the lung with the potential to function as a template for other organ models (e.g., skin, vessels).

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
air–liquid interface cell culture, alveolar-capillary barrier, in vitro cell-stretching model, porous ultra-thin scaffolds, tunable polymeric membranes
in
Advanced Functional Materials
pages
20 pages
publisher
Wiley-VCH Verlag
external identifiers
  • scopus:85069931647
ISSN
1616-301X
DOI
10.1002/adfm.201903114
language
English
LU publication?
yes
id
6e48cb45-d3b3-40d0-bfe5-905fb42fa881
date added to LUP
2019-08-12 10:27:41
date last changed
2019-10-01 10:47:01
@article{6e48cb45-d3b3-40d0-bfe5-905fb42fa881,
  abstract     = {<p>Mechanical stretch under both physiological (breathing) and pathophysiological (ventilator-induced) conditions is known to significantly impact all cellular compartments in the lung, thereby playing a pivotal role in lung growth, regeneration and disease development. In order to evaluate the impact of mechanical forces on the cellular level, in vitro models using lung cells on stretchable membranes have been developed. Only recently have some of these cell-stretching devices become suitable for air–liquid interface cell cultures, which is required to adequately model physiological conditions for the alveolar epithelium. To reach this goal, a multi-functional membrane for cell growth balancing biophysical and mechanical properties is critical to mimic (patho)physiological conditions. In this review, i) the relevance of cyclic mechanical forces in lung biology is elucidated, ii) the physiological range for the key parameters of tissue stretch in the lung is described, and iii) the currently available in vitro cell-stretching devices are discussed. After assessing various polymers, it is concluded that natural-synthetic copolymers are promising candidates for suitable stretchable membranes used in cell-stretching models. This work provides guidance on future developments in biomimetic in vitro models of the lung with the potential to function as a template for other organ models (e.g., skin, vessels).</p>},
  articleno    = {1903114},
  author       = {Doryab, Ali and Tas, Sinem and Taskin, Mehmet Berat and Yang, Lin and Hilgendorff, Anne and Groll, Jürgen and Wagner, Darcy E. and Schmid, Otmar},
  issn         = {1616-301X},
  keyword      = {air–liquid interface cell culture,alveolar-capillary barrier,in vitro cell-stretching model,porous ultra-thin scaffolds,tunable polymeric membranes},
  language     = {eng},
  month        = {01},
  pages        = {20},
  publisher    = {Wiley-VCH Verlag},
  series       = {Advanced Functional Materials},
  title        = {Evolution of Bioengineered Lung Models : Recent Advances and Challenges in Tissue Mimicry for Studying the Role of Mechanical Forces in Cell Biology},
  url          = {http://dx.doi.org/10.1002/adfm.201903114},
  year         = {2019},
}