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A novel combinational approach of microstimulation and bioluminescence imaging to study the mechanisms of action of cerebral electrical stimulation in mice

Arsenault, Dany; Drouin-Ouellet, Janelle LU ; Saint-Pierre, Martine; Petrou, Petros; Dubois, Marilyn; Kriz, Jasna; Barker, Roger A LU ; Cicchetti, Antonio and Cicchetti, Francesca (2015) In Journal of Physiology 593(10). p.78-2257
Abstract

Deep brain stimulation (DBS) is used to treat a number of neurological conditions and is currently being tested to intervene in neuropsychiatric conditions. However, a better understanding of how it works would ensure that side effects could be minimized and benefits optimized. We have thus developed a unique device to perform brain stimulation (BS) in mice and to address fundamental issues related to this methodology in the pre-clinical setting. This new microstimulator prototype was specifically designed to allow simultaneous live bioluminescence imaging of the mouse brain, allowing real time assessment of the impact of stimulation on cerebral tissue. We validated the authenticity of this tool in vivo by analysing the expression of... (More)

Deep brain stimulation (DBS) is used to treat a number of neurological conditions and is currently being tested to intervene in neuropsychiatric conditions. However, a better understanding of how it works would ensure that side effects could be minimized and benefits optimized. We have thus developed a unique device to perform brain stimulation (BS) in mice and to address fundamental issues related to this methodology in the pre-clinical setting. This new microstimulator prototype was specifically designed to allow simultaneous live bioluminescence imaging of the mouse brain, allowing real time assessment of the impact of stimulation on cerebral tissue. We validated the authenticity of this tool in vivo by analysing the expression of toll-like receptor 2 (TLR2), corresponding to the microglial response, in the stimulated brain regions of TLR2-fluc-GFP transgenic mice, which we further corroborated with post-mortem analyses in these animals as well as in human brains of patients who underwent DBS to treat their Parkinson's disease. In the present study, we report on the development of the first BS device that allows for simultaneous live in vivo imaging in mice. This tool opens up a whole new range of possibilities that allow a better understanding of BS and how to optimize its effects through its use in murine models of disease.

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Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
keywords
Action Potentials, Aged, Animals, Autopsy, Brain, Deep Brain Stimulation, Diagnostic Imaging, Disease Models, Animal, Electrodes, Female, Humans, Luminescent Measurements, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Microglia, Middle Aged, Motor Cortex, Parkinson Disease, Signal Transduction, Toll-Like Receptor 2, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Physiology
volume
593
issue
10
pages
22 pages
publisher
The Physiological Society
external identifiers
  • scopus:84929501882
ISSN
1469-7793
DOI
10.1113/jphysiol.2014.287243
language
English
LU publication?
no
id
6e4fa71c-64f1-4e78-97ed-9c9de768b6a9
date added to LUP
2016-11-22 08:59:51
date last changed
2017-09-17 09:32:53
@article{6e4fa71c-64f1-4e78-97ed-9c9de768b6a9,
  abstract     = {<p>Deep brain stimulation (DBS) is used to treat a number of neurological conditions and is currently being tested to intervene in neuropsychiatric conditions. However, a better understanding of how it works would ensure that side effects could be minimized and benefits optimized. We have thus developed a unique device to perform brain stimulation (BS) in mice and to address fundamental issues related to this methodology in the pre-clinical setting. This new microstimulator prototype was specifically designed to allow simultaneous live bioluminescence imaging of the mouse brain, allowing real time assessment of the impact of stimulation on cerebral tissue. We validated the authenticity of this tool in vivo by analysing the expression of toll-like receptor 2 (TLR2), corresponding to the microglial response, in the stimulated brain regions of TLR2-fluc-GFP transgenic mice, which we further corroborated with post-mortem analyses in these animals as well as in human brains of patients who underwent DBS to treat their Parkinson's disease. In the present study, we report on the development of the first BS device that allows for simultaneous live in vivo imaging in mice. This tool opens up a whole new range of possibilities that allow a better understanding of BS and how to optimize its effects through its use in murine models of disease.</p>},
  author       = {Arsenault, Dany and Drouin-Ouellet, Janelle and Saint-Pierre, Martine and Petrou, Petros and Dubois, Marilyn and Kriz, Jasna and Barker, Roger A and Cicchetti, Antonio and Cicchetti, Francesca},
  issn         = {1469-7793},
  keyword      = {Action Potentials,Aged,Animals,Autopsy,Brain,Deep Brain Stimulation,Diagnostic Imaging,Disease Models, Animal,Electrodes,Female,Humans,Luminescent Measurements,Male,Mice,Mice, Inbred C57BL,Mice, Transgenic,Microglia,Middle Aged,Motor Cortex,Parkinson Disease,Signal Transduction,Toll-Like Receptor 2,Comparative Study,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {05},
  number       = {10},
  pages        = {78--2257},
  publisher    = {The Physiological Society},
  series       = {Journal of Physiology},
  title        = {A novel combinational approach of microstimulation and bioluminescence imaging to study the mechanisms of action of cerebral electrical stimulation in mice},
  url          = {http://dx.doi.org/10.1113/jphysiol.2014.287243},
  volume       = {593},
  year         = {2015},
}