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Reelin Immunoreactivity in the Adult Spinal Cord : A Comparative Study in Rodents, Carnivores, and Non-human Primates

Krzyzanowska, Agnieszka LU ; Cabrerizo, Marina ; Clascá, Francisco and Ramos-Moreno, Tania LU orcid (2020) In Frontiers in Neuroanatomy 13.
Abstract

Reelin is a large extracellular matrix (ECM) glycoprotein secreted by several neuronal populations in a specific manner in both the developing and the adult central nervous system. The extent of Reelin protein distribution and its functional role in the adult neocortex is well documented in different mammal models. However, its role in the adult spinal cord has not been well characterized and its distribution in the rodent spinal cord is fragmentary and has not been investigated in carnivores or primates as of yet. To gain insight into which neuronal populations and specific circuits may be influenced by Reelin in the adult spinal cord, we have conducted light and confocal microscopy study analysis of Reelin-immunoreactive cell types in... (More)

Reelin is a large extracellular matrix (ECM) glycoprotein secreted by several neuronal populations in a specific manner in both the developing and the adult central nervous system. The extent of Reelin protein distribution and its functional role in the adult neocortex is well documented in different mammal models. However, its role in the adult spinal cord has not been well characterized and its distribution in the rodent spinal cord is fragmentary and has not been investigated in carnivores or primates as of yet. To gain insight into which neuronal populations and specific circuits may be influenced by Reelin in the adult spinal cord, we have conducted light and confocal microscopy study analysis of Reelin-immunoreactive cell types in the adult spinal cord. Here, we describe and compare Reelin immunoreactive cell type and distribution in the spinal cord of adult non-human primate (macaque monkeys, Macaca mulatta), carnivore (ferret, Mustela putorius) and rodent (rat, Rattus norvegicus). Our results show that in all three species studied, Reelin-immunoreactive neurons are present in the intermediate gray matter, ventricular zone and superficial dorsal horn and intermedio-lateral nucleus, while positive cells in the Clarke nucleus are only found in rats and primates. In addition, Reelin intermediolateral neurons colocalize with choline acetyltransferase (ChAT) only in macaque whilst motor neurons also colocalize Reelin and ChAT in macaque, ferret and rat spinal cord. The different expression patterns might reflect a differential role for Reelin in the pathways involved in the coordination of locomotor activity in the fore- and hind limbs.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
motor neurons, nociception, primates, reelin, spinal cord
in
Frontiers in Neuroanatomy
volume
13
article number
102
publisher
Frontiers Media S. A.
external identifiers
  • scopus:85078482314
  • pmid:31969808
ISSN
1662-5129
DOI
10.3389/fnana.2019.00102
language
English
LU publication?
yes
id
6ef5bcd6-013e-4195-a3ba-2b0d4d5890ef
date added to LUP
2020-02-07 13:59:07
date last changed
2024-04-03 02:39:20
@article{6ef5bcd6-013e-4195-a3ba-2b0d4d5890ef,
  abstract     = {{<p>Reelin is a large extracellular matrix (ECM) glycoprotein secreted by several neuronal populations in a specific manner in both the developing and the adult central nervous system. The extent of Reelin protein distribution and its functional role in the adult neocortex is well documented in different mammal models. However, its role in the adult spinal cord has not been well characterized and its distribution in the rodent spinal cord is fragmentary and has not been investigated in carnivores or primates as of yet. To gain insight into which neuronal populations and specific circuits may be influenced by Reelin in the adult spinal cord, we have conducted light and confocal microscopy study analysis of Reelin-immunoreactive cell types in the adult spinal cord. Here, we describe and compare Reelin immunoreactive cell type and distribution in the spinal cord of adult non-human primate (macaque monkeys, Macaca mulatta), carnivore (ferret, Mustela putorius) and rodent (rat, Rattus norvegicus). Our results show that in all three species studied, Reelin-immunoreactive neurons are present in the intermediate gray matter, ventricular zone and superficial dorsal horn and intermedio-lateral nucleus, while positive cells in the Clarke nucleus are only found in rats and primates. In addition, Reelin intermediolateral neurons colocalize with choline acetyltransferase (ChAT) only in macaque whilst motor neurons also colocalize Reelin and ChAT in macaque, ferret and rat spinal cord. The different expression patterns might reflect a differential role for Reelin in the pathways involved in the coordination of locomotor activity in the fore- and hind limbs.</p>}},
  author       = {{Krzyzanowska, Agnieszka and Cabrerizo, Marina and Clascá, Francisco and Ramos-Moreno, Tania}},
  issn         = {{1662-5129}},
  keywords     = {{motor neurons; nociception; primates; reelin; spinal cord}},
  language     = {{eng}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Neuroanatomy}},
  title        = {{Reelin Immunoreactivity in the Adult Spinal Cord : A Comparative Study in Rodents, Carnivores, and Non-human Primates}},
  url          = {{http://dx.doi.org/10.3389/fnana.2019.00102}},
  doi          = {{10.3389/fnana.2019.00102}},
  volume       = {{13}},
  year         = {{2020}},
}