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Gamma-tubulin coordinates nuclear envelope assembly around chromatin

Rossello Castillo, Catalina Ana LU ; Lindström, Lisa LU ; Glindre, Johan LU ; Eklund, Greta LU and Alvarado-Kristensson, Maria LU (2016) In Heliyon 2(9).
Abstract

The cytosolic role of γ-tubulin as a microtubule organizer has been studied thoroughly, but its nuclear function is poorly understood. Here, we show that γ-tubulin is located throughout the chromatin of demembranated Xenopus laevis sperm and, as the nucleus is formed, γ-tubulin recruits lamin B3 and nuclear membranes. Immunodepletion of γ-tubulin impairs X. laevis assembly of both the lamina and the nuclear membrane. During nuclear formation in mammalian cell lines, γ-tubulin establishes a cellular protein boundary around chromatin that coordinates nuclear assembly of the daughter nuclei. Furthermore, expression of a γ-tubulin mutant that lacks the DNA-binding domain forms chromatin-empty nuclear like structures and demonstrate that a... (More)

The cytosolic role of γ-tubulin as a microtubule organizer has been studied thoroughly, but its nuclear function is poorly understood. Here, we show that γ-tubulin is located throughout the chromatin of demembranated Xenopus laevis sperm and, as the nucleus is formed, γ-tubulin recruits lamin B3 and nuclear membranes. Immunodepletion of γ-tubulin impairs X. laevis assembly of both the lamina and the nuclear membrane. During nuclear formation in mammalian cell lines, γ-tubulin establishes a cellular protein boundary around chromatin that coordinates nuclear assembly of the daughter nuclei. Furthermore, expression of a γ-tubulin mutant that lacks the DNA-binding domain forms chromatin-empty nuclear like structures and demonstrate that a constant interplay between the chromatin-associated and the cytosolic pools of γ-tubulin is required and, when the balance between pools is impaired, aberrant nuclei are formed. We therefore propose that the nuclear protein meshwork formed by γ-tubulin around chromatin coordinates nuclear formation in eukaryotic cells.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biological sciences, Cell biology
in
Heliyon
volume
2
issue
9
publisher
Elsevier
external identifiers
  • scopus:84991329275
ISSN
2405-8440
DOI
10.1016/j.heliyon.2016.e00166
language
English
LU publication?
yes
id
6f535b02-5150-4aa8-89c7-905e5c20dec8
date added to LUP
2016-11-23 13:37:46
date last changed
2017-02-26 04:43:01
@article{6f535b02-5150-4aa8-89c7-905e5c20dec8,
  abstract     = {<p>The cytosolic role of γ-tubulin as a microtubule organizer has been studied thoroughly, but its nuclear function is poorly understood. Here, we show that γ-tubulin is located throughout the chromatin of demembranated Xenopus laevis sperm and, as the nucleus is formed, γ-tubulin recruits lamin B3 and nuclear membranes. Immunodepletion of γ-tubulin impairs X. laevis assembly of both the lamina and the nuclear membrane. During nuclear formation in mammalian cell lines, γ-tubulin establishes a cellular protein boundary around chromatin that coordinates nuclear assembly of the daughter nuclei. Furthermore, expression of a γ-tubulin mutant that lacks the DNA-binding domain forms chromatin-empty nuclear like structures and demonstrate that a constant interplay between the chromatin-associated and the cytosolic pools of γ-tubulin is required and, when the balance between pools is impaired, aberrant nuclei are formed. We therefore propose that the nuclear protein meshwork formed by γ-tubulin around chromatin coordinates nuclear formation in eukaryotic cells.</p>},
  articleno    = {e00166},
  author       = {Rossello Castillo, Catalina Ana and Lindström, Lisa and Glindre, Johan and Eklund, Greta and Alvarado-Kristensson, Maria},
  issn         = {2405-8440},
  keyword      = {Biological sciences,Cell biology},
  language     = {eng},
  month        = {09},
  number       = {9},
  publisher    = {Elsevier},
  series       = {Heliyon},
  title        = {Gamma-tubulin coordinates nuclear envelope assembly around chromatin},
  url          = {http://dx.doi.org/10.1016/j.heliyon.2016.e00166},
  volume       = {2},
  year         = {2016},
}